Construct: sgRNA BRDN0001147880

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTACCTTGAAGGTGAACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TYRO3 (7301)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76227

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 41564259 - TYRO3 NNNACCTTGAAGGTGAACAG NGG 0 1.0 Tier I
2 NC_000008.11 8 103975426 - RIMS2 NNNACCCTGATGGTGAACAG NGG 2 0.2115 Tier I
3 NC_000010.11 10 53809346 - PCDH15 NNNACCTTGAGGGTGAAGAG NGG 2 0.0867 Tier I
4 NC_000005.10 5 159972349 - ADRA1B NNNAGCTTGAAGGTGAAGAG NGG 2 0.08 Tier I
5 NC_000012.12 12 128589458 - TMEM132C NNNACCTGGAAGATGAACAG NGG 2 0.6769 Tier II
6 NC_000020.11 20 19976344 - RIN2 NNNACCTTGAGGGAGAACAG NGG 2 0.4024 Tier II
7 NC_000002.12 2 230441927 - SP100 NNNATCTTGAAGGTGAAAAG NGG 2 0.3427 Tier II
8 NC_000023.11 X 2398128 + DHRSX NNNCCCTTGTAGGTGAACAG NGG 2 0.3114 Tier II
9 NC_000024.10 Y 2398128 + DHRSX NNNCCCTTGTAGGTGAACAG NGG 2 0.3114 Tier II
10 NC_000009.12 9 122177055 + MORN5 NNNACCTAGAAGGTCAACAG NGG 2 0.2182 Tier II
11 NC_000003.12 3 151543541 - IGSF10 NNNATCTTGCAGGTGAACAG NGG 2 0.2121 Tier II
12 NC_000013.11 13 94324456 + GPC6 NNNCCCTTGAAGGTGAACAC NGG 2 0.1513 Tier II
13 NC_000016.10 16 2567227 + PDPK1 NNNACCTTGAAGGTGAACCT NGG 2 0.1448 Tier II
14 NC_000004.12 4 6604001 + MAN2B2 NNNACCTTGGAGGTGAGCAG NGG 2 0.098 Tier II
15 NC_000002.12 2 115031460 - DPP10 NNNCCCTTGAAGGTCAACAG NGG 2 0.0963 Tier II
16 NC_000017.11 17 79486426 - RBFOX3 NNNCCCTTGAAGGTCAACAG NGG 2 0.0963 Tier II
17 NC_000011.10 11 66727260 - SPTBN2 NNNTCCTTGAAGGTGAAGAG NGG 2 0.0848 Tier II
18 NC_000008.11 8 105677624 + ZFPM2 NNNACCTTGAATGTGAACAG NGC 2 0.0085 Tier II
19 NC_000004.12 4 6433288 + PPP2R2C NNNACCTAGAAGGGGAACAG NGG 2 0.0 Tier II
20 NC_000021.9 21 29751465 - GRIK1 NNNACCTTGAAGGGGAAAAG NGG 2 0.0 Tier II
21 NC_000007.14 7 96527147 + SEM1 NNNACCTTGAAGGGGAAGAG NGG 2 0.0 Tier II
22 NC_000006.12 6 134447541 - LINC01010 NNNACCTGGAAGGTGAACAA NGG 2 0.6875 Tier III
23 NC_000006.12 6 134447541 - CT69 NNNACCTGGAAGGTGAACAA NGG 2 0.6875 Tier III
24 NC_000009.12 9 108381486 + LOC105376214 NNNACCTTGAAGATGAAAAG NGG 2 0.497 Tier III
25 NC_000002.12 2 230441927 - LOC101928816 NNNATCTTGAAGGTGAAAAG NGG 2 0.3427 Tier III
26 NC_000012.12 12 95343237 - LOC105369917 NNNACCTGGAAGGTGAACAG NAG 2 0.1901 Tier III
27 NC_000003.12 3 98718682 + ST3GAL6-AS1 NNNACCTTGAAGGTGGACAA NGG 2 0.1803 Tier III
28 NC_000004.12 4 38635718 - KLF3-AS1 NNNACCTTAAAGGTGAACAG NAG 2 0.1667 Tier III
29 NC_000016.10 16 2632966 - PDPK2P NNNACCTTGAAGGTGAACCT NGG 2 0.1448 Tier III
30 NC_000008.11 8 92714018 - FLJ46284 NNNACCTTGCTGGTGAACAG NGG 2 0.1026 Tier III
31 NC_000004.12 4 187576615 + LINC02492 NNNACCTTGAAAGTGAACAG NGA 2 0.0648 Tier III
32 NC_000004.12 4 187576615 + LOC105377604 NNNACCTTGAAAGTGAACAG NGA 2 0.0648 Tier III
33 NC_000012.12 12 118880563 + LOC105370019 NNNACCTTGCAGGTGAGCAG NGG 2 0.0588 Tier III
34 NC_000021.9 21 29751465 - GRIK1-AS1 NNNACCTTGAAGGGGAAAAG NGG 2 0.0 Tier III
35 NC_000007.14 7 96527147 + LOC105375412 NNNACCTTGAAGGGGAAGAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 119804843 - Tyro3 NNNACCTTGAAGGCGAACAA NGG 2 0.2679 Tier I
2 NC_000076.6 10 12735191 - Utrn NNNACCTTGAAGCTGAACAG NTG 2 0.0164 Tier I
3 NC_000071.6 5 130262807 - Tyw1 NNNACCTTGAAGATGAACAG NGG 1 0.9231 Tier II
4 NC_000076.6 10 33416378 - Trdn NNNACTTTGAAAGTGAACAG NGG 2 0.8667 Tier II
5 NC_000080.6 14 22181728 - Lrmda NNNACCTTGTAGGTGAACAA NGG 2 0.8272 Tier II
6 NC_000068.7 2 139495600 - Sptlc3 NNNACCTAGAAGGTAAACAG NGG 2 0.7529 Tier II
7 NC_000074.6 8 28868257 - Unc5d NNNACTCTGAAGGTGAACAG NGG 2 0.6384 Tier II
8 NC_000081.6 15 87680138 - Tafa5 NNNACCTTGTAGGAGAACAG NGG 2 0.5462 Tier II
9 NC_000070.6 4 81367109 - Mpdz NNNACATTGAAGGTGAACTG NGG 2 0.5 Tier II
10 NC_000081.6 15 5030119 - C7 NNNATCTTGAAGGAGAACAG NGG 2 0.3939 Tier II
11 NC_000075.6 9 51234190 - Pou2af1 NNNACCTTGACGGTAAACAG NGG 2 0.3765 Tier II
12 NC_000069.6 3 157076645 - Negr1 NNNACCATGAAGCTGAACAG NGG 2 0.3684 Tier II
13 NC_000075.6 9 64119247 - Lctl NNNAACTTGAAGGAGAACAG NGG 2 0.3537 Tier II
14 NC_000067.6 1 149905675 + Pla2g4a NNNTCCTTGAAGGTGAAAAG NGG 2 0.3427 Tier II
15 NC_000080.6 14 23655803 + Kcnma1 NNNACCTTGAATGTGAACTG NGG 2 0.2071 Tier II
16 NC_000082.6 16 55932851 - Cep97 NNNACCTTGAAAGTGAAGAG NGG 2 0.1244 Tier II
17 NC_000074.6 8 128376317 - Nrp1 NNNAACTTGAAGGTGGACAG NGG 2 0.1099 Tier II
18 NC_000081.6 15 98124318 - Pfkm NNNACCTTGGAGGTGAGCAG NGG 2 0.098 Tier II
19 NC_000067.6 1 125562209 + Slc35f5 NNNTCCTTGAAGGTGAAGAG NGG 2 0.0848 Tier II
20 NC_000067.6 1 43170170 - Fhl2 NNNTCCTTGAAGGTGATCAG NGG 2 0.0848 Tier II
21 NC_000067.6 1 91929760 - Hdac4 NNNACCTTGAAGGAGAAGAG NGG 2 0.0825 Tier II
22 NC_000084.6 18 12282267 + Ankrd29 NNNACCTTGAAGGTCACCAG NGG 2 0.0481 Tier II
23 NC_000079.6 13 92299623 - Msh3 NNNACCTTGAAGGTTACCAG NGG 2 0.0252 Tier II
24 NC_000072.6 6 32568913 + Plxna4 NNNCCCTTGAAGGTGAACAG NGA 2 0.0245 Tier II
25 NC_000067.6 1 84193358 - Pid1 NNNACCTTGGAGGTGAACAG NTG 2 0.0216 Tier II
26 NC_000067.6 1 106710458 + Bcl2 NNNACCTTGATGGTGAACAG NGA 2 0.0214 Tier II
27 NC_000079.6 13 56112900 - Macroh2a1 NNNACCTTGAAGGTGACCAG NTG 2 0.0069 Tier II
28 NC_000079.6 13 75090555 + Pcsk1 NNNAACTTGAAGGTGCACAG NGG 2 0.0 Tier II
29 NC_000073.6 7 112258810 + Mical2 NNNACCTTGAAAGGGAACAG NGG 2 0.0 Tier II
30 NC_000075.6 9 72990061 - Ccpg1 NNNACCTTGAAGGTACACAG NGG 2 0.0 Tier II
31 NC_000074.6 8 84776791 + Nfix NNNACCTTGAAGGTGAACAG NTC 2 0.0 Tier II
32 NC_000077.6 11 50862671 + Grm6 NNNACCTTGAAGGTGCTCAG NGG 2 0.0 Tier II
33 NC_000068.7 2 16017286 + Malrd1 NNNATCTTGAAGGGGAACAG NGG 2 0.0 Tier II
34 NC_000071.6 5 30059743 - Tyms NNNATCTTGAAGGGGAACAG NGG 2 0.0 Tier II
35 NC_000086.7 X 9475291 + Cybb NNNATCTTGAAGGGGAACAG NGG 2 0.0 Tier II
36 NC_000086.7 X 166669055 + Gm1720 NNNACCATGAAGATGAACAG NGG 2 0.8077 Tier III
37 NC_000078.6 12 77579784 - Gm35240 NNNACATTGAGGGTGAACAG NGG 2 0.6036 Tier III
38 NC_000086.7 X 75678149 + LOC115489104 NNNATCTTGAAGATGAACAG NGG 2 0.5874 Tier III
39 NC_000075.6 9 47302425 + Gm31816 NNNTCCATGAAGGTGAACAG NGG 2 0.5568 Tier III
40 NC_000072.6 6 68025370 - Igk NNNACCTAGAAGCTGAACAG NGG 2 0.3368 Tier III
41 NC_000068.7 2 115331529 - 4930528P14Rik NNNACCTTGTAGGTCAACAG NGG 2 0.2406 Tier III
42 NC_000081.6 15 10742104 - 4930556M19Rik NNNACCTAGAAGGTGAACAG NGA 2 0.0556 Tier III
43 NC_000079.6 13 56112900 - Gm31485 NNNACCTTGAAGGTGACCAG NTG 2 0.0069 Tier III
44 NC_000074.6 8 35235145 - Gm34597 NNNACCTTGAAGGGGAGCAG NGG 2 0.0 Tier III
45 NC_000072.6 6 147207509 - E330012B07Rik NNNACCTTGAAGGTGCACAG NGG 1 0.0 Tier III
46 NC_000085.6 19 3154453 - 1700030N03Rik NNNATCTTGAAGGGGAACAG NGG 2 0.0 Tier III
47 NC_000085.6 19 3154453 - Gm42068 NNNATCTTGAAGGGGAACAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)