Construct: sgRNA BRDN0001148165
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGGTGATAGATTTAGCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL2 (8999)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77588
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
75603822 |
- |
CDKL2 |
NNNGGTGATAGATTTAGCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
170355847 |
+ |
MYO3B |
NNNGCTGATAGATTTAGCAA |
NGG |
1 |
0.7857 |
Tier II |
| 3 |
NC_000005.10 |
5 |
157370976 |
+ |
CYFIP2 |
NNNACTGATAGATTTAGCAA |
NGG |
2 |
0.7071 |
Tier II |
| 4 |
NC_000011.10 |
11 |
32628876 |
+ |
CCDC73 |
NNNGGTAATAGAATTAGCAA |
NGG |
2 |
0.6923 |
Tier II |
| 5 |
NC_000010.11 |
10 |
50190393 |
- |
ASAH2 |
NNNGGTGATACATTTAGCAA |
NGG |
1 |
0.4286 |
Tier II |
| 6 |
NC_000010.11 |
10 |
50751663 |
+ |
ASAH2B |
NNNGGTGATACATTTAGCAA |
NGG |
1 |
0.4286 |
Tier II |
| 7 |
NC_000012.12 |
12 |
70700190 |
- |
PTPRR |
NNNGGTGACAGATTCAGCAA |
NGG |
2 |
0.2517 |
Tier II |
| 8 |
NC_000007.14 |
7 |
76035790 |
- |
STYXL1 |
NNNGGAGATAGAGTTAGCAA |
NGG |
2 |
0.2261 |
Tier II |
| 9 |
NC_000005.10 |
5 |
76721941 |
- |
F2R |
NNNTGTTATAGATTTAGCAA |
NGG |
2 |
0.2078 |
Tier II |
| 10 |
NC_000007.14 |
7 |
71259454 |
+ |
GALNT17 |
NNNGGTGATAGAGTTAGCAG |
NGG |
2 |
0.1995 |
Tier II |
| 11 |
NC_000007.14 |
7 |
148198293 |
+ |
CNTNAP2 |
NNNGGTGATAGGTTTGGCAA |
NGG |
2 |
0.1389 |
Tier II |
| 12 |
NC_000009.12 |
9 |
19302863 |
- |
DENND4C |
NNNGGTGAGAGATTTAGCCA |
NGG |
2 |
0.1281 |
Tier II |
| 13 |
NC_000019.10 |
19 |
56417908 |
- |
ZNF583 |
NNNGGTGATAGAATTAGGAA |
NGG |
2 |
0.0923 |
Tier II |
| 14 |
NC_000002.12 |
2 |
84703687 |
+ |
DNAH6 |
NNNAGTGATAGATTTAGCAA |
NGT |
2 |
0.0145 |
Tier II |
| 15 |
NC_000010.11 |
10 |
15833870 |
- |
MINDY3 |
NNNGGTGATACATTTAGCAA |
NGC |
2 |
0.0095 |
Tier II |
| 16 |
NC_000008.11 |
8 |
12724995 |
- |
LONRF1 |
NNNGGTGATAGATTTTACAA |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000012.12 |
12 |
76877778 |
- |
CSRP2 |
NNNGGTGATAGATTTTGAAA |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000005.10 |
5 |
157370976 |
+ |
NIPAL4-DT |
NNNACTGATAGATTTAGCAA |
NGG |
2 |
0.7071 |
Tier III |
| 19 |
NC_000011.10 |
11 |
30726341 |
- |
LINC02859 |
NNNGCTGGTAGATTTAGCAA |
NGG |
2 |
0.5762 |
Tier III |
| 20 |
NC_000004.12 |
4 |
177835323 |
+ |
LINC01098 |
NNNGGTGATTGATTTAGCAA |
NAG |
2 |
0.2288 |
Tier III |
| 21 |
NC_000004.12 |
4 |
177835323 |
+ |
LINC01099 |
NNNGGTGATTGATTTAGCAA |
NAG |
2 |
0.2288 |
Tier III |
| 22 |
NC_000017.11 |
17 |
82456306 |
- |
NARF-AS2 |
NNNGGTGATAGTTTTAGGAA |
NGG |
2 |
0.0444 |
Tier III |
| 23 |
NC_000010.11 |
10 |
15833870 |
- |
LOC124902383 |
NNNGGTGATACATTTAGCAA |
NGC |
2 |
0.0095 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
108957862 |
- |
Wdr25 |
NNNGGTGATAGAGTTAGCAG |
NGG |
2 |
0.1995 |
Tier II |
| 2 |
NC_000073.6 |
7 |
125990597 |
+ |
Gsg1l |
NNNGGTGATAGGTTCAGCAA |
NGG |
2 |
0.197 |
Tier II |
| 3 |
NC_000072.6 |
6 |
126848350 |
+ |
Ndufa9 |
NNNGGTGATGGCTTTAGCAA |
NGG |
2 |
0.1462 |
Tier II |
| 4 |
NC_000071.6 |
5 |
21920601 |
+ |
Reln |
NNNGATGATAGATTTAGCAA |
NGA |
2 |
0.0602 |
Tier II |
| 5 |
NC_000082.6 |
16 |
35728388 |
- |
Slc49a4 |
NNNGGTGTTAGATTTAGCAA |
NGA |
2 |
0.0298 |
Tier II |
| 6 |
NC_000071.6 |
5 |
33150595 |
- |
Slc5a1 |
NNNGGTGATAGAGTGAGCAA |
NGG |
2 |
0.013 |
Tier II |
| 7 |
NC_000077.6 |
11 |
113681535 |
- |
Fam104a |
NNNGGTGATAGATCTAGCAA |
NTG |
2 |
0.0111 |
Tier II |
| 8 |
NC_000082.6 |
16 |
4151430 |
- |
Crebbp |
NNNGGTGATAGATTTTGCAA |
NAG |
2 |
0.0 |
Tier II |
| 9 |
NC_000069.6 |
3 |
17457406 |
- |
Gm30340 |
NNNTGTGATAGAATTAGCAA |
NGG |
2 |
0.2517 |
Tier III |
Other clones with same target sequence:
(none)
