Construct: sgRNA BRDN0001148207

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCCGAGTTGGAGAACAGGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK4 (205)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77693

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 65190804 + AK4 NNNGAGTTGGAGAACAGGCG NGG 0 1.0 Tier I
2 NC_000022.11 22 26541118 + TPST2 NNNGAGTTGGGGAACAGGCG NGA 2 0.0451 Tier I
3 NC_000022.11 22 45325346 + FAM118A NNNGAGTCGGAGAACAGGCA NGG 2 0.9375 Tier II
4 NC_000022.11 22 23278446 + BCR NNNGAGTTGGAGAACAGGCT NGG 1 0.7 Tier II
5 NC_000008.11 8 106649011 + OXR1 NNNGAGTTGAAGAACAGGCT NGG 2 0.6533 Tier II
6 NC_000002.12 2 232134955 + DIS3L2 NNNGACTTGGAGAACAGGCA NGG 2 0.6392 Tier II
7 NC_000001.11 1 83995042 - TTLL7 NNNAAGTTGGAGAACAGGCT NGG 2 0.63 Tier II
8 NC_000018.10 18 9708668 - RAB31 NNNGAGGCGGAGAACAGGCG NGG 2 0.5882 Tier II
9 NC_000007.14 7 27145596 + HOXA3 NNNAAGTTGGGGAACAGGCG NGG 2 0.585 Tier II
10 NC_000007.14 7 27145596 + HOXA6 NNNAAGTTGGGGAACAGGCG NGG 2 0.585 Tier II
11 NC_000013.11 13 113990955 + RASA3 NNNGGGCTGGAGAACAGGCG NGG 2 0.495 Tier II
12 NC_000013.11 13 113991083 + RASA3 NNNGGGCTGGAGAACAGGCG NGG 2 0.495 Tier II
13 NC_000013.11 13 113991211 + RASA3 NNNGGGCTGGAGAACAGGCG NGG 2 0.495 Tier II
14 NC_000013.11 13 113991275 + RASA3 NNNGGGCTGGAGAACAGGCG NGG 2 0.495 Tier II
15 NC_000014.9 14 54431037 - CNIH1 NNNGAGTTGGAGAACAGACT NGG 2 0.4846 Tier II
16 NC_000017.11 17 75342482 - GRB2 NNNGAATTGGAGAACAGCCG NGG 2 0.4762 Tier II
17 NC_000018.10 18 46962213 - KATNAL2 NNNGAGGTGGAGAACAGGCT NGG 2 0.4118 Tier II
18 NC_000018.10 18 46968140 - KATNAL2 NNNGAGGTGGAGAACAGGCT NGG 2 0.4118 Tier II
19 NC_000018.10 18 47021732 - KATNAL2 NNNGAGGTGGAGAACAGGCT NGG 2 0.4118 Tier II
20 NC_000018.10 18 47027647 - KATNAL2 NNNGAGGTGGAGAACAGGCT NGG 2 0.4118 Tier II
21 NC_000001.11 1 19316593 - SLC66A1 NNNGAGTTCGAGAACAGGCT NGG 2 0.3769 Tier II
22 NC_000002.12 2 38904512 + ARHGEF33 NNNGAGTTCGAGAACAGGCT NGG 2 0.3769 Tier II
23 NC_000016.10 16 1389392 + UNKL NNNGAGTTCGAGAACAGGCT NGG 2 0.3769 Tier II
24 NC_000001.11 1 242190688 + PLD5 NNNGAGTTGGAGATCAGGCT NGG 2 0.3733 Tier II
25 NC_000008.11 8 76763429 + ZFHX4 NNNGAGTTGGAGATCAGGCT NGG 2 0.3733 Tier II
26 NC_000001.11 1 42535666 + CCDC30 NNNGAGTTTGAGAACAGGCT NGG 2 0.3733 Tier II
27 NC_000003.12 3 185651420 - IGF2BP2 NNNGAGTTTGAGAACAGGCT NGG 2 0.3733 Tier II
28 NC_000020.11 20 33602020 - CBFA2T2 NNNGAGTTTGAGAACAGGCT NGG 2 0.3733 Tier II
29 NC_000022.11 22 37862048 - EIF3L NNNGAGTTTGAGAACAGGCT NGG 2 0.3733 Tier II
30 NC_000022.11 22 24516172 + UPB1 NNNGAGTTGGAGATCAGTCG NGG 2 0.3556 Tier II
31 NC_000001.11 1 28084821 - EYA3 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
32 NC_000001.11 1 99723322 + FRRS1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
33 NC_000001.11 1 151259793 - PSMD4 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
34 NC_000001.11 1 156822364 + NTRK1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
35 NC_000002.12 2 69324017 - GFPT1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
36 NC_000002.12 2 128149254 - UGGT1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
37 NC_000003.12 3 31871677 + OSBPL10 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
38 NC_000003.12 3 98743232 - ST3GAL6 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
39 NC_000004.12 4 1594649 + FAM53A NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
40 NC_000005.10 5 43309452 - HMGCS1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
41 NC_000005.10 5 135751640 + SLC25A48 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
42 NC_000007.14 7 4919375 + MMD2 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
43 NC_000007.14 7 99269488 - MYH16 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
44 NC_000007.14 7 122876575 - CADPS2 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
45 NC_000007.14 7 152772325 + ACTR3B NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
46 NC_000008.11 8 42962618 - HOOK3 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
47 NC_000008.11 8 102327112 - UBR5 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
48 NC_000009.12 9 37447963 + ZBTB5 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
49 NC_000009.12 9 111551614 - PTGR1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
50 NC_000009.12 9 111551614 - ZNF483 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
51 NC_000010.11 10 14581707 + FAM107B NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
52 NC_000010.11 10 24032459 - KIAA1217 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
53 NC_000010.11 10 102130859 + PPRC1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
54 NC_000011.10 11 89920950 - TRIM49D1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
55 NC_000013.11 13 30731581 - ALOX5AP NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
56 NC_000014.9 14 73446845 + NUMB NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
57 NC_000016.10 16 3838046 - CREBBP NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
58 NC_000016.10 16 23349284 + SCNN1B NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
59 NC_000016.10 16 46593537 + SHCBP1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
60 NC_000016.10 16 56909265 + SLC12A3 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
61 NC_000017.11 17 29500119 + TAOK1 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
62 NC_000019.10 19 34467274 + UBA2 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
63 NC_000019.10 19 35014833 + GRAMD1A NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
64 NC_000019.10 19 35304752 - MAG NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
65 NC_000019.10 19 41004795 + CYP2B6 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
66 NC_000023.11 X 41592057 + CASK NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
67 NC_000023.11 X 48525591 - EBP NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
68 NC_000002.12 2 8729958 + KIDINS220 NNNGAGTTGGAGAACAGACC NGG 2 0.2967 Tier II
69 NC_000015.10 15 88613486 + AEN NNNGAGCTGGAGAACAGGAG NGG 2 0.2946 Tier II
70 NC_000005.10 5 154693037 - LARP1 NNNGAGTTCGAGAACAGCCG NGG 2 0.2564 Tier II
71 NC_000012.12 12 123779253 - DNAH10 NNNGAGTTCGAGAACAGCCG NGG 2 0.2564 Tier II
72 NC_000012.12 12 62554708 - MON2 NNNGAGTTTGAGAACAGCCG NGG 2 0.254 Tier II
73 NC_000019.10 19 17554286 + NIBAN3 NNNGAGTTTGAGAACAGCCG NGG 2 0.254 Tier II
74 NC_000005.10 5 128534851 - FBN2 NNNGAGTTGGAGAGCAGCCG NGG 2 0.2222 Tier II
75 NC_000001.11 1 31041289 - PUM1 NNNGAGTTGGAGAACAGCTG NGG 2 0.2198 Tier II
76 NC_000001.11 1 156764130 - HDGF NNNGAGTTGGAGACCAGGCG NGG 1 0.2143 Tier II
77 NC_000010.11 10 48529280 + ARHGAP22 NNNGAGTTGGAGACCAGGCA NGG 2 0.2009 Tier II
78 NC_000001.11 1 237209659 + RYR2 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
79 NC_000003.12 3 55997977 - ERC2 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
80 NC_000004.12 4 168818404 + PALLD NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
81 NC_000005.10 5 54617774 - SNX18 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
82 NC_000005.10 5 95936589 + ELL2 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
83 NC_000006.12 6 32309162 - TSBP1 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
84 NC_000007.14 7 74513818 + GTF2IRD1 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
85 NC_000008.11 8 25223527 - DOCK5 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
86 NC_000011.10 11 47295224 - MADD NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
87 NC_000014.9 14 63687794 + SGPP1 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
88 NC_000015.10 15 71473270 - THSD4 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
89 NC_000017.11 17 50672671 + ABCC3 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
90 NC_000020.11 20 34956861 + MYH7B NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
91 NC_000021.9 21 36358562 + MORC3 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
92 NC_000023.11 X 43729690 - MAOA NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
93 NC_000023.11 X 54618701 + GNL3L NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier II
94 NC_000002.12 2 39665530 + TMEM178A NNNGAGTTGGAGAAAAGTCG NGG 2 0.1481 Tier II
95 NC_000003.12 3 7296861 - GRM7 NNNGAGTTGGAGACCAGTCG NGG 2 0.1429 Tier II
96 NC_000007.14 7 101527183 - COL26A1 NNNGAGTTGGAGACCAGTCG NGG 2 0.1429 Tier II
97 NC_000022.11 22 17396046 + CECR2 NNNGAGTTGGAGACCAGTCG NGG 2 0.1429 Tier II
98 NC_000017.11 17 67117763 - HELZ NNNGAGTTCGAGACCAGGCG NGG 2 0.1154 Tier II
99 NC_000021.9 21 39387072 - GET1 NNNGAGTTCGAGACCAGGCG NGG 2 0.1154 Tier II
100 NC_000021.9 21 39387072 - GET1-SH3BGR NNNGAGTTCGAGACCAGGCG NGG 2 0.1154 Tier II
101 NC_000010.11 10 126645374 + C10orf90 NNNGAGTTTGAGACCAGGCG NGG 2 0.1143 Tier II
102 NC_000007.14 7 5705441 - RNF216 NNNGAGTTGGAGAACAGGAG NAG 2 0.1111 Tier II
103 NC_000019.10 19 45691734 - SNRPD2 NNNGAGTTTGAGAACGGGCG NGG 2 0.1026 Tier II
104 NC_000004.12 4 134090571 + PABPC4L NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
105 NC_000005.10 5 172966374 - RPL26L1 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
106 NC_000007.14 7 135608514 + NUP205 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
107 NC_000011.10 11 72042920 + NUMA1 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
108 NC_000013.11 13 41309401 - MTRF1 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
109 NC_000015.10 15 99236600 - TTC23 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
110 NC_000017.11 17 67732786 - NOL11 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
111 NC_000019.10 19 29819494 - CCNE1 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
112 NC_000022.11 22 39962230 + GRAP2 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
113 NC_000022.11 22 39962230 + TMA7B NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
114 NC_000023.11 X 6202550 - NLGN4X NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
115 NC_000023.11 X 101463970 - ARMCX4 NNNGAGTTGGAGACCAGCCG NGG 2 0.102 Tier II
116 NC_000004.12 4 139319095 + NAA15 NNNGAGTTGGAGACCAGGCC NGG 2 0.0918 Tier II
117 NC_000012.12 12 123466848 - SNRNP35 NNNGAGTTGGAGACCAGGCC NGG 2 0.0918 Tier II
118 NC_000015.10 15 78210826 - ACSBG1 NNNGAGTTGGAGACCAGGCC NGG 2 0.0918 Tier II
119 NC_000005.10 5 67010893 + MAST4 NNNGACTTGGAGAACAGGGG NGG 2 0.0852 Tier II
120 NC_000017.11 17 31345758 + NF1 NNNGAGTTGGAGAATAGGCG NGG 1 0.0667 Tier II
121 NC_000022.11 22 35836002 - RBFOX2 NNNGAGTTGGAGGATAGGCG NGG 2 0.0435 Tier II
122 NC_000020.11 20 35174353 + PROCR NNNGAGATGGAGAACAGGCG NTG 2 0.0341 Tier II
123 NC_000020.11 20 35174353 + MMP24-AS1-EDEM2 NNNGAGATGGAGAACAGGCG NTG 2 0.0341 Tier II
124 NC_000005.10 5 13774287 + DNAH5 NNNGAGTTGGAGAAAAGGGG NGG 2 0.0278 Tier II
125 NC_000003.12 3 105455896 + ALCAM NNNGAGTTGGAGAACAGGCT NGC 2 0.0156 Tier II
126 NC_000019.10 19 13298035 - CACNA1A NNNGAGTTGGAGAACAGGCT NGC 2 0.0156 Tier II
127 NC_000023.11 X 72358129 - HDAC8 NNNGAGTTGGAGACCAGGCG NGA 2 0.0149 Tier II
128 NC_000005.10 5 168390672 - WWC1 NNNGAGTTAGAGAACAGGCG NGT 2 0.0104 Tier II
129 NC_000015.10 15 74327697 + CCDC33 NNNGAGTTGGAGAACAGGAG NGT 2 0.0069 Tier II
130 NC_000009.12 9 96110315 - LOC158434 NNNGAGTTGGAGAACAGGCT NGG 1 0.7 Tier III
131 NC_000008.11 8 106649011 + OXR1-AS1 NNNGAGTTGAAGAACAGGCT NGG 2 0.6533 Tier III
132 NC_000018.10 18 9708668 - LOC105371981 NNNGAGGCGGAGAACAGGCG NGG 2 0.5882 Tier III
133 NC_000007.14 7 27145596 + HOXA-AS3 NNNAAGTTGGGGAACAGGCG NGG 2 0.585 Tier III
134 NC_000003.12 3 160751571 + PPM1L-DT NNNGAGTTCGAGAACAGGCT NGG 2 0.3769 Tier III
135 NC_000002.12 2 78919281 + LOC105374822 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier III
136 NC_000002.12 2 156150416 + LINC01876 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier III
137 NC_000008.11 8 33788190 - LOC105379364 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier III
138 NC_000014.9 14 53883928 + LOC107984676 NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier III
139 NC_000012.12 12 123779253 - LOC105370044 NNNGAGTTCGAGAACAGCCG NGG 2 0.2564 Tier III
140 NC_000001.11 1 114844612 - NR1H5P NNNGAGTTTGAGAACAGCCG NGG 2 0.254 Tier III
141 NC_000007.14 7 118431786 + LOC105375471 NNNGTGTTGGAGAACAGGCC NGG 2 0.1558 Tier III
142 NC_000006.12 6 32309162 - TSBP1-AS1 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier III
143 NC_000016.10 16 58768782 - LOC107984867 NNNGAGTTGGAGACCAGGCT NGG 2 0.15 Tier III
144 NC_000006.12 6 146919957 - STXBP5-AS1 NNNGAGTTGGAGAACAAGGG NGG 2 0.1167 Tier III
145 NC_000007.14 7 38328568 + TRG NNNGAGTTGGAGAACAGGAG NAG 2 0.1111 Tier III
146 NC_000012.12 12 31616201 - AK4P3 NNNGAGTTGGAGAATAGGCG NGG 1 0.0667 Tier III
147 NC_000017.11 17 31345758 + AK4P1 NNNGAGTTGGAGAATAGGCG NGG 1 0.0667 Tier III
148 NC_000005.10 5 55552316 - AK4P2 NNNGAGTTGGAGAATAGCCG NGG 2 0.0317 Tier III
149 NC_000004.12 4 151027191 + AK4P6 NNNGAGTTGGAGAATAGGTG NGG 2 0.0308 Tier III
150 NC_000012.12 12 118131983 - LOC101928274 NNNGAGTTGGAGAACAGGCT NGC 2 0.0156 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 112308149 - Tpst2 NNNGAGTTGGGGAACAGGCG NGC 2 0.0144 Tier I
2 NC_000073.6 7 101715694 + Clpb NNNGAGTTGGAGAACAGTCA NGG 2 0.625 Tier II
3 NC_000076.6 10 26299653 + L3mbtl3 NNNGAGATGGAGAACAGGCT NGG 2 0.6125 Tier II
4 NC_000076.6 10 92999433 + Cfap54 NNNGGGTTGGAGAACAGGCT NGG 2 0.504 Tier II
5 NC_000078.6 12 85630652 + Jdp2 NNNGAGTTGGAGAACAGTCT NGG 2 0.4667 Tier II
6 NC_000073.6 7 112136490 - Dkk3 NNNGAGTTGGAGGACAGGCT NGG 2 0.4565 Tier II
7 NC_000081.6 15 99998809 - Larp4 NNNGAGTTGGAGAACAGCCA NGG 2 0.4464 Tier II
8 NC_000073.6 7 72119579 - Mctp2 NNNGAGTTTGAGAACAGGCT NGG 2 0.3733 Tier II
9 NC_000086.7 X 85075324 - Dmd NNNGAGTTTGAGAACAGGCT NGG 2 0.3733 Tier II
10 NC_000077.6 11 74383790 + Rap1gap2 NNNGAGCTGGAGATCAGGCG NGG 2 0.3667 Tier II
11 NC_000077.6 11 29383454 - Ccdc88a NNNGAGTTGGAGAACAGCCT NGG 2 0.3333 Tier II
12 NC_000070.6 4 137893728 - Ece1 NNNGAGTGGGAGAACAGGAG NGG 2 0.3143 Tier II
13 NC_000072.6 6 28740830 - Snd1 NNNGAGTTGGAGAACAGTCC NGG 2 0.2857 Tier II
14 NC_000084.6 18 58206924 - Fbn2 NNNGAGTTGGAGAGCAGCCG NGG 2 0.2222 Tier II
15 NC_000070.6 4 21962864 - Faxc NNNGAGTTGGAGAACAGGCT NAG 2 0.1815 Tier II
16 NC_000079.6 13 104473238 + Adamts6 NNNGAGTTGGAGAACAGGGA NGG 2 0.1172 Tier II
17 NC_000086.7 X 161112866 + Scml2 NNNGAGTTGGAGAACAGGAG NAG 2 0.1111 Tier II
18 NC_000074.6 8 104838807 - Ces2b NNNGAGTTGGAGAAAAGGAG NGG 2 0.0952 Tier II
19 NC_000081.6 15 30731604 + Ctnnd2 NNNGAGTGGGAGAACAGGGG NGG 2 0.0917 Tier II
20 NC_000078.6 12 37544650 - Agmo NNNGAGTTGGGGAACAGGGG NGG 2 0.0813 Tier II
21 NC_000078.6 12 119211105 - Itgb8 NNNGAGTTGGAGAACAGGGG NTG 2 0.0049 Tier II
22 NC_000078.6 12 5602026 - Gm32398 NNNGAGTTGGAAAACAAGCG NGG 2 0.8711 Tier III
23 NC_000069.6 3 137983532 - Gm19708 NNNGAGGTGGAGAGCAGGCG NGG 2 0.2745 Tier III
24 NC_000071.6 5 136927943 - 4933404O12Rik NNNGAGTTCCAGAACAGGCG NGG 2 0.2154 Tier III
25 NC_000081.6 15 30731604 + Gm41283 NNNGAGTGGGAGAACAGGGG NGG 2 0.0917 Tier III
26 NC_000078.6 12 99031621 - Gm40554 NNNGAGTTGGAGAACAGGGG NAG 2 0.0324 Tier III
27 NC_000071.6 5 66664247 + Uchl1os NNNGAGTTGGAGAAGAGGCG NGC 2 0.0011 Tier III
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Other clones with same target sequence:

(none)