Construct: sgRNA BRDN0001148341
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCATGCTTTCCGTGTGGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CRKL (1399)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77509
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
20934024 |
- |
CRKL |
NNNATGCTTTCCGTGTGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
35244071 |
+ |
SCUBE3 |
NNNATGCTTCCCTTGTGGTG |
NGG |
2 |
0.16 |
Tier I |
| 3 |
NC_000005.10 |
5 |
59171694 |
- |
PDE4D |
NNNATGCTTTCCATGTGGTT |
NGG |
2 |
0.6462 |
Tier II |
| 4 |
NC_000014.9 |
14 |
54723304 |
+ |
SAMD4A |
NNNATGCTTTCCATGTGGTT |
NGG |
2 |
0.6462 |
Tier II |
| 5 |
NC_000010.11 |
10 |
15225479 |
+ |
FAM171A1 |
NNNATGCTTTCCGTGTTTTG |
NGG |
2 |
0.1667 |
Tier II |
| 6 |
NC_000004.12 |
4 |
7510997 |
+ |
SORCS2 |
NNNCTGCTTTCCCTGTGGTG |
NGG |
2 |
0.1486 |
Tier II |
| 7 |
NC_000009.12 |
9 |
124029978 |
- |
LHX2 |
NNNATGCTTTCCCTGGGGTG |
NGG |
2 |
0.1457 |
Tier II |
| 8 |
NC_000001.11 |
1 |
163070000 |
+ |
RGS4 |
NNNATGCTTTCCGTGTGGTG |
NTG |
1 |
0.039 |
Tier II |
| 9 |
NC_000017.11 |
17 |
57269901 |
+ |
MSI2 |
NNNCTGCTTTCCGTGTGGTG |
NGA |
2 |
0.0245 |
Tier II |
| 10 |
NC_000010.11 |
10 |
15225479 |
+ |
LOC105376433 |
NNNATGCTTTCCGTGTTTTG |
NGG |
2 |
0.1667 |
Tier III |
| 11 |
NC_000006.12 |
6 |
35244071 |
+ |
SCUBE3-AS1 |
NNNATGCTTCCCTTGTGGTG |
NGG |
2 |
0.16 |
Tier III |
| 12 |
NC_000004.12 |
4 |
7510997 |
+ |
LOC124900655 |
NNNCTGCTTTCCCTGTGGTG |
NGG |
2 |
0.1486 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
49129935 |
+ |
Tenm3 |
NNNATGCTTTCCATATGGTG |
NGG |
2 |
0.8688 |
Tier II |
| 2 |
NC_000068.7 |
2 |
91968002 |
+ |
Dgkz |
NNNATGGTCTCCGTGTGGTG |
NGG |
2 |
0.4344 |
Tier II |
| 3 |
NC_000083.6 |
17 |
78906473 |
- |
Sult6b1 |
NNNATGGTTTCCATGTGGTG |
NGG |
2 |
0.4344 |
Tier II |
| 4 |
NC_000084.6 |
18 |
84582522 |
+ |
Zfp407 |
NNNATGCTTTATGTGTGGTG |
NGG |
2 |
0.4038 |
Tier II |
| 5 |
NC_000067.6 |
1 |
74056655 |
- |
Tns1 |
NNNATGCTTTCCCTGTGGTA |
NGG |
2 |
0.3947 |
Tier II |
| 6 |
NC_000067.6 |
1 |
43523218 |
- |
Nck2 |
NNNATGCTTTCAGTGTGCTG |
NGG |
2 |
0.3401 |
Tier II |
| 7 |
NC_000068.7 |
2 |
106360176 |
+ |
Dcdc5 |
NNNATGCTGTCTGTGTGGTG |
NGG |
2 |
0.3333 |
Tier II |
| 8 |
NC_000077.6 |
11 |
72723312 |
+ |
Ankfy1 |
NNNATGCTGTCCGTGTGCTG |
NGG |
2 |
0.2948 |
Tier II |
| 9 |
NC_000078.6 |
12 |
37566377 |
+ |
Agmo |
NNNATGCTTTCCATGTTGTG |
NGG |
2 |
0.2308 |
Tier II |
| 10 |
NC_000080.6 |
14 |
35137614 |
+ |
Grid1 |
NNNAGGCTTTCCGTGTGGAG |
NGG |
2 |
0.1829 |
Tier II |
| 11 |
NC_000075.6 |
9 |
118984731 |
+ |
Ctdspl |
NNNATGCTTTCCGTGTCCTG |
NGG |
2 |
0.112 |
Tier II |
| 12 |
NC_000080.6 |
14 |
35053995 |
+ |
Grid1 |
NNNATGCTTTCCCTGTGGGG |
NGG |
2 |
0.1053 |
Tier II |
| 13 |
NC_000079.6 |
13 |
81345429 |
+ |
Adgrv1 |
NNNAAGCTTTCCGTTTGGTG |
NGG |
2 |
0.0714 |
Tier II |
| 14 |
NC_000077.6 |
11 |
35817112 |
- |
Rars |
NNNGTGCTTTCCGTGTGGTG |
NGA |
2 |
0.0434 |
Tier II |
| 15 |
NC_000067.6 |
1 |
152842115 |
- |
Smg7 |
NNNATGCTTTCCGTGTAGTG |
NGT |
2 |
0.0151 |
Tier II |
| 16 |
NC_000081.6 |
15 |
83530217 |
- |
Bik |
NNNATGCTTTCCGGGGGGTG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000081.6 |
15 |
8583026 |
+ |
Gm46508 |
NNNAAGCTCTCCGTGTGGTG |
NGG |
2 |
0.4615 |
Tier III |
| 18 |
NC_000080.6 |
14 |
35137614 |
+ |
Gm41133 |
NNNAGGCTTTCCGTGTGGAG |
NGG |
2 |
0.1829 |
Tier III |
Other clones with same target sequence:
(none)
