Construct: sgRNA BRDN0001148375
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATTGAATTGGCTACAGGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OXSR1 (9943)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77750
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
38223879 |
+ |
OXSR1 |
NNNTGAATTGGCTACAGGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
70151711 |
+ |
COL19A1 |
NNNTGAATTGGCTACAGGGA |
NGG |
1 |
0.9375 |
Tier II |
| 3 |
NC_000017.11 |
17 |
1472830 |
+ |
MYO1C |
NNNTGAAGTGGCTACAGGGA |
NGG |
2 |
0.6875 |
Tier II |
| 4 |
NC_000005.10 |
5 |
16709256 |
+ |
MYO10 |
NNNTGAGTTGGCTACAGGAG |
NGG |
2 |
0.5042 |
Tier II |
| 5 |
NC_000004.12 |
4 |
62003828 |
+ |
ADGRL3 |
NNNAGAATTGGCTCCAGGGG |
NGG |
2 |
0.102 |
Tier II |
| 6 |
NC_000005.10 |
5 |
61652227 |
+ |
LINC03122 |
NNNTCACTTGGCTACAGGGG |
NGG |
2 |
0.3437 |
Tier III |
| 7 |
NC_000010.11 |
10 |
120282575 |
- |
LOC105378515 |
NNNTGAATTGGCTCCAGGTG |
NGG |
2 |
0.1429 |
Tier III |
| 8 |
NC_000016.10 |
16 |
9561592 |
+ |
LOC132205950 |
NNNTGAACTGGCTAGAGGGG |
NGG |
2 |
0.05 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
100202065 |
+ |
Ror1 |
NNNTGAATTGGCTACAAGTG |
NGG |
2 |
0.6222 |
Tier II |
| 2 |
NC_000075.6 |
9 |
21827225 |
+ |
Dock6 |
NNNTGAAATGGATACAGGGG |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000072.6 |
6 |
121455089 |
- |
Iqsec3 |
NNNTGAATTGGCCACAGGAG |
NGG |
2 |
0.5639 |
Tier II |
| 4 |
NC_000074.6 |
8 |
126301642 |
+ |
Slc35f3 |
NNNTGATTAGGCTACAGGGG |
NGG |
2 |
0.4062 |
Tier II |
| 5 |
NC_000069.6 |
3 |
59215178 |
+ |
Med12l |
NNNTGAATTGGCTGCAGGTG |
NGG |
2 |
0.3111 |
Tier II |
| 6 |
NC_000068.7 |
2 |
103285948 |
- |
Ehf |
NNNTGAATTGGGAACAGGGG |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000067.6 |
1 |
86530201 |
+ |
Ptma |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier II |
| 8 |
NC_000069.6 |
3 |
8882037 |
- |
Mrps28 |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier II |
| 9 |
NC_000079.6 |
13 |
62779303 |
+ |
Gm5141 |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier II |
| 10 |
NC_000068.7 |
2 |
167957345 |
- |
Ptpn1 |
NNNTGAATTGGCTAGAGTGG |
NGG |
2 |
0.0333 |
Tier II |
| 11 |
NC_000071.6 |
5 |
31911001 |
- |
Babam2 |
NNNTGAATTGGCTACAGGAG |
NGC |
2 |
0.0159 |
Tier II |
| 12 |
NC_000084.6 |
18 |
37375426 |
- |
Gm37013 |
NNNTGAATTGGCTAAAGGGG |
NGA |
2 |
0.0154 |
Tier II |
| 13 |
NC_000084.6 |
18 |
37375426 |
- |
Gm38666 |
NNNTGAATTGGCTAAAGGGG |
NGA |
2 |
0.0154 |
Tier II |
| 14 |
NC_000084.6 |
18 |
37375426 |
- |
Gm38667 |
NNNTGAATTGGCTAAAGGGG |
NGA |
2 |
0.0154 |
Tier II |
| 15 |
NC_000077.6 |
11 |
72770266 |
- |
Ankfy1 |
NNNTGAATTGGCTACTGGGG |
NGG |
1 |
0.0 |
Tier II |
| 16 |
NC_000069.6 |
3 |
133956140 |
- |
Gm26691 |
NNNTGAACTGGCTACAGAGG |
NGG |
2 |
0.6923 |
Tier III |
| 17 |
NC_000069.6 |
3 |
8882037 |
- |
Gm15466 |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier III |
| 18 |
NC_000069.6 |
3 |
124386187 |
+ |
Gm4617 |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier III |
| 19 |
NC_000070.6 |
4 |
44123732 |
+ |
Ptma-ps2 |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier III |
| 20 |
NC_000070.6 |
4 |
44123732 |
+ |
LOC115489981 |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier III |
| 21 |
NC_000074.6 |
8 |
89146559 |
- |
Gm6625 |
NNNTGAATTGGCAACATGGG |
NGG |
2 |
0.1731 |
Tier III |
Other clones with same target sequence:
(none)
