Construct: sgRNA BRDN0001148437

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATTGACAAAGACGAGAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGKA (1606)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76606

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 55941281 - DGKA NNNTGACAAAGACGAGAAGT NGG 0 1.0 Tier I
2 NC_000001.11 1 101888392 + OLFM3 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
3 NC_000002.12 2 9928135 - TAF1B NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
4 NC_000004.12 4 123242491 + AFG2A NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
5 NC_000005.10 5 145881912 + GRXCR2 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
6 NC_000009.12 9 28631518 - LINGO2 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
7 NC_000009.12 9 122540387 - OR1J2 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
8 NC_000010.11 10 115754444 - ATRNL1 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
9 NC_000011.10 11 4868960 + MMP26 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
10 NC_000011.10 11 85350680 - DLG2 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
11 NC_000011.10 11 85466581 - DLG2 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
12 NC_000018.10 18 68749047 - CCDC102B NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier II
13 NC_000001.11 1 63503784 - ITGB3BP NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
14 NC_000004.12 4 167122 - ZNF718 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
15 NC_000004.12 4 169454072 + NEK1 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
16 NC_000007.14 7 146161904 + CNTNAP2 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
17 NC_000009.12 9 85761516 + AGTPBP1 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
18 NC_000012.12 12 50977388 + SLC11A2 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
19 NC_000013.11 13 69915485 + KLHL1 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
20 NC_000015.10 15 28156034 - HERC2 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
21 NC_000015.10 15 29416389 + ENTREP2 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
22 NC_000018.10 18 74752646 - ZNF407 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier II
23 NC_000004.12 4 120806591 + PRDM5 NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier II
24 NC_000005.10 5 145926499 - GRXCR2 NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier II
25 NC_000010.11 10 52025598 - PRKG1 NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier II
26 NC_000010.11 10 88351501 - RNLS NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier II
27 NC_000012.12 12 28478354 + CCDC91 NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier II
28 NC_000002.12 2 153252743 - GALNT13 NNNTGACAAAAACCAGAAGT NGG 2 0.4286 Tier II
29 NC_000010.11 10 102965418 + CNNM2 NNNAGTCAAAGACGAGAAGT NGG 2 0.3401 Tier II
30 NC_000015.10 15 50656909 - TRPM7 NNNTGACAATGAAGAGAAGT NGG 2 0.3394 Tier II
31 NC_000015.10 15 50656909 - LOC128092252 NNNTGACAATGAAGAGAAGT NGG 2 0.3394 Tier II
32 NC_000014.9 14 74410116 + SYNDIG1L NNNTGAGAAAGATGAGAAGT NGG 2 0.3294 Tier II
33 NC_000017.11 17 896669 - NXN NNNTGACTAAGATGAGAAGT NGG 2 0.3 Tier II
34 NC_000005.10 5 134212290 - PPP2CA NNNTGACAAAGACAAGAAGT NAG 2 0.1944 Tier II
35 NC_000006.12 6 39680688 + KIF6 NNNTGACAAAGACTAGAAAT NGG 2 0.1905 Tier II
36 NC_000013.11 13 66557866 + PCDH9 NNNAGACAAAGAAGAGAAGT NGG 2 0.1832 Tier II
37 NC_000004.12 4 106087841 + TBCK NNNTGACAAAAACGAGCAGT NGG 2 0.1765 Tier II
38 NC_000018.10 18 45537402 + SLC14A2 NNNTGACAAAGACGTGAAGA NGG 2 0.1125 Tier II
39 NC_000007.14 7 138698046 + SVOPL NNNTGAAAAAGAGGAGAAGT NGG 2 0.1023 Tier II
40 NC_000016.10 16 57643444 - ADGRG1 NNNTGACAAAGGGGAGAAGT NGG 2 0.0985 Tier II
41 NC_000004.12 4 2691029 - FAM193A NNNTTACAAAGACGAGAAGT NAG 2 0.0778 Tier II
42 NC_000001.11 1 101888392 + DNAJA1P5 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
43 NC_000004.12 4 107961549 + LOC107986298 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
44 NC_000009.12 9 28631518 - LOC105376003 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
45 NC_000010.11 10 4899155 + AKR1C6P NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
46 NC_000011.10 11 59544805 - LOC102723575 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
47 NC_000012.12 12 106547182 - LOC100287944 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
48 NC_000019.10 19 41491251 + PCAT19 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
49 NC_000023.11 X 132319308 + RAP2C-AS1 NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
50 NC_000024.10 Y 7307711 - PRKY NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
51 NC_000024.10 Y 21999433 + OFD1P8Y NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
52 NC_000024.10 Y 22586963 - OFD1P9Y NNNTGACAAAAACAAGAAGT NGG 2 0.75 Tier III
53 NC_000001.11 1 63503784 - LOC102724319 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
54 NC_000002.12 2 40693291 + LOC105374497 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
55 NC_000005.10 5 126295362 + LOC124901056 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
56 NC_000007.14 7 20884693 - LINC01162 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
57 NC_000008.11 8 7164205 + LOC105377803 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
58 NC_000008.11 8 8123278 - FAM85B NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
59 NC_000008.11 8 111001822 - LINC01608 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
60 NC_000009.12 9 85761516 + LOC100419824 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
61 NC_000013.11 13 66281911 - LOC124903179 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
62 NC_000018.10 18 44446896 + LINC01478 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
63 NC_000019.10 19 27943754 - LINC02987 NNNTGACAAAAACGAGAAAT NGG 2 0.7143 Tier III
64 NC_000001.11 1 56049510 - LOC105378737 NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier III
65 NC_000010.11 10 88351501 - LOC101929727 NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier III
66 NC_000015.10 15 79557601 - TFDP1P3 NNNTGACAAAGACAAGAAAT NGG 2 0.5357 Tier III
67 NC_000009.12 9 32347176 - LOC107987059 NNNTGAGAAAGACGAGAAAT NGG 2 0.3361 Tier III
68 NC_000010.11 10 64055997 - LOC124902439 NNNTGCCAAAGATGAGAAGT NGG 2 0.3182 Tier III
69 NC_000021.9 21 16229454 + MIR99AHG NNNTGACAAAAACGAGCAGT NGG 2 0.1765 Tier III
70 NC_000004.12 4 159883210 - LOC107986324 NNNTGAGAAAGAGGAGAAGT NGG 2 0.0642 Tier III
71 NC_000010.11 10 9263609 + LOC101928272 NNNTGACAAAGAGGAGGAGT NGG 2 0.0241 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 138342201 - Pvrig NNNTGAGAAAGACGAGAGGT NGG 2 0.1882 Tier I
2 NC_000068.7 2 168639932 - Atp9a NNNGGACAAAGACGTGAAGT NGG 2 0.125 Tier I
3 NC_000080.6 14 67266205 - Ebf2 NNNTGGCAAAGACAAGAAGT NGG 2 0.5357 Tier II
4 NC_000086.7 X 37159413 + Akap14 NNNTGACAGAGATGAGAAGT NGG 2 0.4667 Tier II
5 NC_000072.6 6 65622158 - Tnip3 NNNAGAAAAAGACGAGAAGT NGG 2 0.3571 Tier II
6 NC_000067.6 1 129948477 + Thsd7b NNNAGACAAAGACAAGAAGT NGG 2 0.3571 Tier II
7 NC_000077.6 11 108176702 - Prkca NNNTGAAAAAGAAGAGAAGT NGG 2 0.2885 Tier II
8 NC_000068.7 2 127957642 - Acoxl NNNTGACAAGGACCAGAAGT NGG 2 0.2381 Tier II
9 NC_000085.6 19 24851894 - Pgm5 NNNTGACACAGACTAGAAGT NGG 2 0.1524 Tier II
10 NC_000079.6 13 81611149 - Adgrv1 NNNAGACAAAGACTAGAAGT NGG 2 0.127 Tier II
11 NC_000072.6 6 29500028 + Kcp NNNTGACAAAGACCAGAAGT NAG 2 0.1111 Tier II
12 NC_000072.6 6 80025690 - Lrrtm4 NNNTGACAAAGACAAGAAGT NGA 2 0.0521 Tier II
13 NC_000071.6 5 69145699 + Kctd8 NNNTGACAAAGACTAGACGT NGG 2 0.0508 Tier II
14 NC_000086.7 X 37159413 + Gm39503 NNNTGACAGAGATGAGAAGT NGG 2 0.4667 Tier III
15 NC_000077.6 11 108176702 - Gm11655 NNNTGAAAAAGAAGAGAAGT NGG 2 0.2885 Tier III
16 NC_000079.6 13 60431926 + Gm35333 NNNTGACAAAGACAAGAAGT NAG 2 0.1944 Tier III
17 NC_000081.6 15 40128199 - Gm33251 NNNTAACAAAGACGATAAGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)