Construct: sgRNA BRDN0001148569
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCTGAAACATCCAAACAT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- CAMK2G (818)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000010.11 | 10 | 73860835 | - | CAMK2G | NNNCTGAAACATCCAAACAT | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 211674413 | - | NEK2 | NNNCTGAAACATCCAAACAT | NGT | 1 | 0.0161 | Tier I |
3 | NC_000023.11 | X | 41787238 | - | CASK | NNNCTGAAACATCCACACAT | NGT | 2 | 0.0 | Tier I |
4 | NC_000010.11 | 10 | 96220822 | - | BLNK | NNNCTGAAACATCTAAACAT | NGG | 1 | 0.7333 | Tier II |
5 | NC_000003.12 | 3 | 26683364 | + | LRRC3B | NNNCTGAAATATCCAAATAT | NGG | 2 | 0.605 | Tier II |
6 | NC_000016.10 | 16 | 7028797 | + | RBFOX1 | NNNATGGAACATCCAAACAT | NGG | 2 | 0.5944 | Tier II |
7 | NC_000009.12 | 9 | 132614919 | - | DDX31 | NNNCTGAATTATCCAAACAT | NGG | 2 | 0.5647 | Tier II |
8 | NC_000007.14 | 7 | 157910882 | - | PTPRN2 | NNNATTAAACATCCAAACAT | NGG | 2 | 0.5614 | Tier II |
9 | NC_000008.11 | 8 | 103716010 | - | RIMS2 | NNNATTAAACATCCAAACAT | NGG | 2 | 0.5614 | Tier II |
10 | NC_000001.11 | 1 | 201685511 | - | NAV1 | NNNCAGAAACACCCAAACAT | NGG | 2 | 0.4737 | Tier II |
11 | NC_000012.12 | 12 | 64326063 | + | C12orf56 | NNNCTGAAACAACCAAAAAT | NGG | 2 | 0.4308 | Tier II |
12 | NC_000002.12 | 2 | 211676082 | - | ERBB4 | NNNTTGAAACATCCAAACTT | NGG | 2 | 0.4308 | Tier II |
13 | NC_000001.11 | 1 | 16582086 | - | NBPF1 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
14 | NC_000001.11 | 1 | 16588391 | - | NBPF1 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
15 | NC_000001.11 | 1 | 120440149 | + | NBPF8 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
16 | NC_000001.11 | 1 | 120446501 | + | NBPF8 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
17 | NC_000001.11 | 1 | 120809218 | + | NBPF26 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
18 | NC_000001.11 | 1 | 146141150 | - | NBPF10 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
19 | NC_000001.11 | 1 | 146963764 | + | NBPF12 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
20 | NC_000001.11 | 1 | 146970054 | + | NBPF12 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
21 | NC_000001.11 | 1 | 148123395 | - | NBPF11 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
22 | NC_000001.11 | 1 | 148592098 | - | NBPF14 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
23 | NC_000001.11 | 1 | 149078549 | - | NBPF9 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
24 | NC_000001.11 | 1 | 149479549 | + | NBPF19 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
25 | NT_187366.1 | 1 | 25833 | - | LOC124905564 | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier II |
26 | NC_000007.14 | 7 | 148700676 | - | CUL1 | NNNCTGAAACCTCCAAAAAT | NGG | 2 | 0.2154 | Tier II |
27 | NC_000023.11 | X | 100373983 | + | PCDH19 | NNNCTGAAACATCCAAAAGT | NGG | 2 | 0.2019 | Tier II |
28 | NC_000012.12 | 12 | 66797027 | - | GRIP1 | NNNCTGAAACATCCAACCAT | NGG | 1 | 0.1765 | Tier II |
29 | NC_000004.12 | 4 | 166035272 | - | TLL1 | NNNCTGAAACATACAAACAT | NAG | 2 | 0.0997 | Tier II |
30 | NC_000003.12 | 3 | 195127982 | + | XXYLT1 | NNNCTTAAACATCCAAACAT | NCG | 2 | 0.0714 | Tier II |
31 | NC_000017.11 | 17 | 8456172 | - | NDEL1 | NNNCTGAAACAGGCAAACAT | NGG | 2 | 0.0682 | Tier II |
32 | NC_000004.12 | 4 | 69958044 | - | CSN2 | NNNCTGAAATATCCAAACAT | NGA | 2 | 0.0654 | Tier II |
33 | NC_000023.11 | X | 118643374 | + | DOCK11 | NNNCTGAAACTTCCAAACAG | NGG | 2 | 0.0543 | Tier II |
34 | NC_000002.12 | 2 | 211500056 | + | ERBB4 | NNNCTGAAACAACCAAACAT | NTG | 2 | 0.0312 | Tier II |
35 | NC_000002.12 | 2 | 25839570 | + | ASXL2 | NNNCTGAAACATCCAAACTT | NTG | 2 | 0.021 | Tier II |
36 | NC_000011.10 | 11 | 132930690 | + | OPCML | NNNCTGAAACATCAAAACAT | NGT | 2 | 0.0056 | Tier II |
37 | NC_000001.11 | 1 | 156464769 | + | MEF2D | NNNCTGAAACATCCACACAT | NGT | 2 | 0.0 | Tier II |
38 | NC_000009.12 | 9 | 22100377 | - | CDKN2B-AS1 | NNNATGAAACATCCAAAAAT | NGG | 2 | 0.4534 | Tier III |
39 | NC_000001.11 | 1 | 145587429 | - | NBPF25P | NNNCTGAAGCATCCAAATAT | NGG | 2 | 0.4286 | Tier III |
40 | NC_000005.10 | 5 | 74399221 | + | LINC01331 | NNNCTCAAACATCCAAACTT | NGG | 2 | 0.3671 | Tier III |
41 | NC_000010.11 | 10 | 25686359 | - | LINC00836 | NNNCTGAAACAAACAAACAT | NGG | 2 | 0.3077 | Tier III |
42 | NC_000002.12 | 2 | 49369362 | + | LOC105374595 | NNNCAGAAACATCCAAACGT | NGG | 2 | 0.1875 | Tier III |
43 | NC_000004.12 | 4 | 139596796 | - | LOC105377622 | NNNCTTAAACATCCAAACAG | NGG | 2 | 0.1176 | Tier III |
44 | NC_000009.12 | 9 | 26138655 | - | LOC107987027 | NNNCTGAAACATCCAAACAT | NGT | 1 | 0.0161 | Tier III |
45 | NC_000002.12 | 2 | 131178750 | - | NEK2P4 | NNNCTGAAAAATCCAAACAT | NGT | 2 | 0.014 | Tier III |
46 | NC_000014.9 | 14 | 18699782 | - | NEK2P3 | NNNCTGAAAAATCCAAACAT | NGT | 2 | 0.014 | Tier III |
47 | NC_000014.9 | 14 | 18890228 | - | NEK2P1 | NNNCTGAAAAATCCAAACAT | NGT | 2 | 0.014 | Tier III |
48 | NC_000014.9 | 14 | 19629203 | + | LOC107984133 | NNNCTGAAAAATCCAAACAT | NGT | 2 | 0.014 | Tier III |
49 | NC_000022.11 | 22 | 15612899 | - | NEK2P2 | NNNCTGAAAAATCCAAACAT | NGT | 2 | 0.014 | Tier III |
50 | NC_000012.12 | 12 | 16973713 | - | LOC105369677 | NNNCTGAAACATGGAAACAT | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000080.6 | 14 | 20779209 | - | Camk2g | NNNCTGAAACATCCAAACAT | NGG | 0 | 1.0 | Tier I |
2 | NC_000067.6 | 1 | 184929327 | - | Mark1 | NNNCTGAACCATCCTAACAT | NGG | 2 | 0.1143 | Tier I |
3 | NC_000067.6 | 1 | 191822248 | + | Nek2 | NNNCTGAAACATCCAAACAT | NGT | 1 | 0.0161 | Tier I |
4 | NC_000079.6 | 13 | 76806155 | - | Mctp1 | NNNCTGAAAAATCCAAACAT | NGG | 1 | 0.8667 | Tier II |
5 | NC_000073.6 | 7 | 19762942 | + | Bcam | NNNCTAAAACAACCAAACAT | NGG | 2 | 0.8 | Tier II |
6 | NC_000078.6 | 12 | 103088553 | + | Unc79 | NNNCTGAAACATTTAAACAT | NGG | 2 | 0.5133 | Tier II |
7 | NC_000082.6 | 16 | 81378348 | - | Ncam2 | NNNCTGAAACAGCCAAACAT | NGG | 1 | 0.5 | Tier II |
8 | NC_000069.6 | 3 | 104486950 | - | Lrig2 | NNNCTCAAACATTCAAACAT | NGG | 2 | 0.4773 | Tier II |
9 | NC_000080.6 | 14 | 34997516 | - | Grid1 | NNNCTGATACACCCAAACAT | NGG | 2 | 0.406 | Tier II |
10 | NC_000067.6 | 1 | 68569125 | + | Erbb4 | NNNCTGAAGCATCCAAACTT | NGG | 2 | 0.359 | Tier II |
11 | NC_000079.6 | 13 | 61738971 | + | Gm9595 | NNNCTGAAACATCCAAATTT | NGG | 2 | 0.3462 | Tier II |
12 | NC_000082.6 | 16 | 33757177 | + | Heg1 | NNNCTGTGACATCCAAACAT | NGG | 2 | 0.3208 | Tier II |
13 | NC_000083.6 | 17 | 68313470 | + | L3mbtl4 | NNNCAGAAACAGCCAAACAT | NGG | 2 | 0.25 | Tier II |
14 | NC_000070.6 | 4 | 15921981 | + | Decr1 | NNNCTAAAACATGCAAACAT | NGG | 2 | 0.1364 | Tier II |
15 | NC_000082.6 | 16 | 72745900 | + | Robo1 | NNNCTGAGACATCCAACCAT | NGG | 2 | 0.1294 | Tier II |
16 | NC_000078.6 | 12 | 37334292 | + | Agmo | NNNCTGAAACATCCAAGCTT | NGG | 2 | 0.095 | Tier II |
17 | NC_000069.6 | 3 | 157286564 | + | Negr1 | NNNCTGAAACTTCCAAACAT | NAG | 2 | 0.0798 | Tier II |
18 | NC_000086.7 | X | 147139043 | - | Htr2c | NNNCTGAAACAGCCAAACAC | NGG | 2 | 0.0455 | Tier II |
19 | NC_000076.6 | 10 | 27230403 | + | Lama2 | NNNCTGAAACATCCAAACAT | NGT | 1 | 0.0161 | Tier II |
20 | NC_000084.6 | 18 | 23592812 | - | Dtna | NNNCTGAAACATCTAAACAT | NGT | 2 | 0.0118 | Tier II |
21 | NC_000072.6 | 6 | 96336166 | - | Tafa1 | NNNCTGAAACATCCAATCAT | NTG | 2 | 0.0052 | Tier II |
22 | NC_000069.6 | 3 | 36029035 | + | Ccdc144b | NNNATGAAACATCCACACAT | NGG | 2 | 0.0 | Tier II |
23 | NC_000067.6 | 1 | 11819444 | + | A830018L16Rik | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
24 | NC_000067.6 | 1 | 157236919 | - | Rasal2 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
25 | NC_000068.7 | 2 | 110686627 | - | Ano3 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
26 | NC_000069.6 | 3 | 3623029 | - | Hnf4g | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
27 | NC_000069.6 | 3 | 159861613 | - | Wls | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
28 | NC_000071.6 | 5 | 48758202 | - | Kcnip4 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
29 | NC_000072.6 | 6 | 62248839 | - | Ccser1 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
30 | NC_000073.6 | 7 | 120263984 | + | Abca14 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
31 | NC_000074.6 | 8 | 54904864 | + | Gpm6a | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
32 | NC_000075.6 | 9 | 19501997 | + | Olfr851 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
33 | NC_000075.6 | 9 | 55873227 | - | Scaper | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
34 | NC_000075.6 | 9 | 111242606 | - | Mlh1 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
35 | NC_000076.6 | 10 | 45429759 | + | Lin28b | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
36 | NC_000078.6 | 12 | 34358491 | - | Hdac9 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
37 | NC_000078.6 | 12 | 78270962 | - | Gphn | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
38 | NC_000078.6 | 12 | 101660423 | + | Tc2n | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
39 | NC_000079.6 | 13 | 68621204 | - | Adcy2 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
40 | NC_000082.6 | 16 | 31731337 | + | Dlg1 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
41 | NC_000082.6 | 16 | 49129931 | + | Myh15 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
42 | NC_000084.6 | 18 | 78298049 | + | Slc14a2 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
43 | NC_000085.6 | 19 | 16298171 | + | Gnaq | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier II |
44 | NC_000070.6 | 4 | 144943707 | + | Vps13d | NNNCTGAAACATCCATACTT | NGG | 2 | 0.0 | Tier II |
45 | NC_000072.6 | 6 | 21525600 | + | Kcnd2 | NNNCTGAAACATCCATACTT | NGG | 2 | 0.0 | Tier II |
46 | NC_000076.6 | 10 | 32691395 | - | Nkain2 | NNNCTGAAACATCCATACTT | NGG | 2 | 0.0 | Tier II |
47 | NC_000084.6 | 18 | 53910733 | + | Csnk1g3 | NNNCTGAAACATCCATACTT | NGG | 2 | 0.0 | Tier II |
48 | NC_000076.6 | 10 | 34076796 | + | Trappc3l | NNNCTGAAGCATCCACACAT | NGG | 2 | 0.0 | Tier II |
49 | NC_000074.6 | 8 | 58717810 | - | Galntl6 | NNNCTGAAGCATCCATACAT | NGG | 2 | 0.0 | Tier II |
50 | NC_000071.6 | 5 | 90722544 | - | Gm36084 | NNNCTGAAAAATTCAAACAT | NGG | 2 | 0.6067 | Tier III |
51 | NC_000075.6 | 9 | 113451542 | + | Gm39438 | NNNTTGAAACATCCAAACTT | NGG | 2 | 0.4308 | Tier III |
52 | NC_000068.7 | 2 | 48139490 | - | Gm13481 | NNNCTGAAGCATCCAAACTT | NGG | 2 | 0.359 | Tier III |
53 | NC_000073.6 | 7 | 25961661 | + | Cyp2b28-ps | NNNCTGAAGCATCCAAACTT | NGG | 2 | 0.359 | Tier III |
54 | NC_000074.6 | 8 | 7457832 | + | Gm30954 | NNNCTGAAGCATCCAAACTT | NGG | 2 | 0.359 | Tier III |
55 | NC_000070.6 | 4 | 52048207 | - | C630028M04Rik | NNNCTGAAACATCCAAATTT | NGG | 2 | 0.3462 | Tier III |
56 | NC_000079.6 | 13 | 61855088 | + | Gm7240 | NNNCTGAAACATCCAAATTT | NGG | 2 | 0.3462 | Tier III |
57 | NC_000079.6 | 13 | 52123796 | + | 4921525O09Rik | NNNCAGAAACCTCCAAACAT | NGG | 2 | 0.2 | Tier III |
58 | NC_000067.6 | 1 | 51540262 | + | Gm17767 | NNNCTGAAAAATCCTAACAT | NGG | 2 | 0.1733 | Tier III |
59 | NC_000076.6 | 10 | 92262139 | + | Gm20757 | NNNCTGAAACATCCAAGCTT | NGG | 2 | 0.095 | Tier III |
60 | NC_000073.6 | 7 | 38499785 | + | Gm6819 | NNNCTGAAACATCCAAACAT | NAT | 2 | 0.0 | Tier III |
61 | NC_000070.6 | 4 | 50900746 | + | Gm42287 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier III |
62 | NC_000072.6 | 6 | 41399927 | + | Tcrb | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier III |
63 | NC_000072.6 | 6 | 60399415 | - | A530053G22Rik | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier III |
64 | NC_000080.6 | 14 | 90252089 | - | 4930474H20Rik | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier III |
65 | NC_000086.7 | X | 155763502 | - | Gm15155 | NNNCTGAAACATCCACACTT | NGG | 2 | 0.0 | Tier III |
66 | NC_000081.6 | 15 | 16602865 | + | Gm2611 | NNNCTGAAACATCCATACAT | NGG | 1 | 0.0 | Tier III |
67 | NC_000070.6 | 4 | 144943707 | + | Gm42344 | NNNCTGAAACATCCATACTT | NGG | 2 | 0.0 | Tier III |
68 | NC_000070.6 | 4 | 89025933 | - | Gm34568 | NNNCTGAAGCATCCACACAT | NGG | 2 | 0.0 | Tier III |
69 | NC_000072.6 | 6 | 68761614 | - | Igk | NNNCTGAAGCATCCACACAT | NGG | 2 | 0.0 | Tier III |
70 | NC_000080.6 | 14 | 109448721 | + | Gm30216 | NNNCTGAAGCATCCACACAT | NGG | 2 | 0.0 | Tier III |