Construct: sgRNA BRDN0001148569

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTCTGAAACATCCAAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2G (818)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76238

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 73860835 - CAMK2G NNNCTGAAACATCCAAACAT NGG 0 1.0 Tier I
2 NC_000001.11 1 211674413 - NEK2 NNNCTGAAACATCCAAACAT NGT 1 0.0161 Tier I
3 NC_000023.11 X 41787238 - CASK NNNCTGAAACATCCACACAT NGT 2 0.0 Tier I
4 NC_000010.11 10 96220822 - BLNK NNNCTGAAACATCTAAACAT NGG 1 0.7333 Tier II
5 NC_000003.12 3 26683364 + LRRC3B NNNCTGAAATATCCAAATAT NGG 2 0.605 Tier II
6 NC_000016.10 16 7028797 + RBFOX1 NNNATGGAACATCCAAACAT NGG 2 0.5944 Tier II
7 NC_000009.12 9 132614919 - DDX31 NNNCTGAATTATCCAAACAT NGG 2 0.5647 Tier II
8 NC_000007.14 7 157910882 - PTPRN2 NNNATTAAACATCCAAACAT NGG 2 0.5614 Tier II
9 NC_000008.11 8 103716010 - RIMS2 NNNATTAAACATCCAAACAT NGG 2 0.5614 Tier II
10 NC_000001.11 1 201685511 - NAV1 NNNCAGAAACACCCAAACAT NGG 2 0.4737 Tier II
11 NC_000012.12 12 64326063 + C12orf56 NNNCTGAAACAACCAAAAAT NGG 2 0.4308 Tier II
12 NC_000002.12 2 211676082 - ERBB4 NNNTTGAAACATCCAAACTT NGG 2 0.4308 Tier II
13 NC_000001.11 1 16582086 - NBPF1 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
14 NC_000001.11 1 16588391 - NBPF1 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
15 NC_000001.11 1 120440149 + NBPF8 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
16 NC_000001.11 1 120446501 + NBPF8 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
17 NC_000001.11 1 120809218 + NBPF26 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
18 NC_000001.11 1 146141150 - NBPF10 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
19 NC_000001.11 1 146963764 + NBPF12 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
20 NC_000001.11 1 146970054 + NBPF12 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
21 NC_000001.11 1 148123395 - NBPF11 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
22 NC_000001.11 1 148592098 - NBPF14 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
23 NC_000001.11 1 149078549 - NBPF9 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
24 NC_000001.11 1 149479549 + NBPF19 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
25 NT_187366.1 1 25833 - LOC124905564 NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier II
26 NC_000007.14 7 148700676 - CUL1 NNNCTGAAACCTCCAAAAAT NGG 2 0.2154 Tier II
27 NC_000023.11 X 100373983 + PCDH19 NNNCTGAAACATCCAAAAGT NGG 2 0.2019 Tier II
28 NC_000012.12 12 66797027 - GRIP1 NNNCTGAAACATCCAACCAT NGG 1 0.1765 Tier II
29 NC_000004.12 4 166035272 - TLL1 NNNCTGAAACATACAAACAT NAG 2 0.0997 Tier II
30 NC_000003.12 3 195127982 + XXYLT1 NNNCTTAAACATCCAAACAT NCG 2 0.0714 Tier II
31 NC_000017.11 17 8456172 - NDEL1 NNNCTGAAACAGGCAAACAT NGG 2 0.0682 Tier II
32 NC_000004.12 4 69958044 - CSN2 NNNCTGAAATATCCAAACAT NGA 2 0.0654 Tier II
33 NC_000023.11 X 118643374 + DOCK11 NNNCTGAAACTTCCAAACAG NGG 2 0.0543 Tier II
34 NC_000002.12 2 211500056 + ERBB4 NNNCTGAAACAACCAAACAT NTG 2 0.0312 Tier II
35 NC_000002.12 2 25839570 + ASXL2 NNNCTGAAACATCCAAACTT NTG 2 0.021 Tier II
36 NC_000011.10 11 132930690 + OPCML NNNCTGAAACATCAAAACAT NGT 2 0.0056 Tier II
37 NC_000001.11 1 156464769 + MEF2D NNNCTGAAACATCCACACAT NGT 2 0.0 Tier II
38 NC_000009.12 9 22100377 - CDKN2B-AS1 NNNATGAAACATCCAAAAAT NGG 2 0.4534 Tier III
39 NC_000001.11 1 145587429 - NBPF25P NNNCTGAAGCATCCAAATAT NGG 2 0.4286 Tier III
40 NC_000005.10 5 74399221 + LINC01331 NNNCTCAAACATCCAAACTT NGG 2 0.3671 Tier III
41 NC_000010.11 10 25686359 - LINC00836 NNNCTGAAACAAACAAACAT NGG 2 0.3077 Tier III
42 NC_000002.12 2 49369362 + LOC105374595 NNNCAGAAACATCCAAACGT NGG 2 0.1875 Tier III
43 NC_000004.12 4 139596796 - LOC105377622 NNNCTTAAACATCCAAACAG NGG 2 0.1176 Tier III
44 NC_000009.12 9 26138655 - LOC107987027 NNNCTGAAACATCCAAACAT NGT 1 0.0161 Tier III
45 NC_000002.12 2 131178750 - NEK2P4 NNNCTGAAAAATCCAAACAT NGT 2 0.014 Tier III
46 NC_000014.9 14 18699782 - NEK2P3 NNNCTGAAAAATCCAAACAT NGT 2 0.014 Tier III
47 NC_000014.9 14 18890228 - NEK2P1 NNNCTGAAAAATCCAAACAT NGT 2 0.014 Tier III
48 NC_000014.9 14 19629203 + LOC107984133 NNNCTGAAAAATCCAAACAT NGT 2 0.014 Tier III
49 NC_000022.11 22 15612899 - NEK2P2 NNNCTGAAAAATCCAAACAT NGT 2 0.014 Tier III
50 NC_000012.12 12 16973713 - LOC105369677 NNNCTGAAACATGGAAACAT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 20779209 - Camk2g NNNCTGAAACATCCAAACAT NGG 0 1.0 Tier I
2 NC_000067.6 1 184929327 - Mark1 NNNCTGAACCATCCTAACAT NGG 2 0.1143 Tier I
3 NC_000067.6 1 191822248 + Nek2 NNNCTGAAACATCCAAACAT NGT 1 0.0161 Tier I
4 NC_000079.6 13 76806155 - Mctp1 NNNCTGAAAAATCCAAACAT NGG 1 0.8667 Tier II
5 NC_000073.6 7 19762942 + Bcam NNNCTAAAACAACCAAACAT NGG 2 0.8 Tier II
6 NC_000078.6 12 103088553 + Unc79 NNNCTGAAACATTTAAACAT NGG 2 0.5133 Tier II
7 NC_000082.6 16 81378348 - Ncam2 NNNCTGAAACAGCCAAACAT NGG 1 0.5 Tier II
8 NC_000069.6 3 104486950 - Lrig2 NNNCTCAAACATTCAAACAT NGG 2 0.4773 Tier II
9 NC_000080.6 14 34997516 - Grid1 NNNCTGATACACCCAAACAT NGG 2 0.406 Tier II
10 NC_000067.6 1 68569125 + Erbb4 NNNCTGAAGCATCCAAACTT NGG 2 0.359 Tier II
11 NC_000079.6 13 61738971 + Gm9595 NNNCTGAAACATCCAAATTT NGG 2 0.3462 Tier II
12 NC_000082.6 16 33757177 + Heg1 NNNCTGTGACATCCAAACAT NGG 2 0.3208 Tier II
13 NC_000083.6 17 68313470 + L3mbtl4 NNNCAGAAACAGCCAAACAT NGG 2 0.25 Tier II
14 NC_000070.6 4 15921981 + Decr1 NNNCTAAAACATGCAAACAT NGG 2 0.1364 Tier II
15 NC_000082.6 16 72745900 + Robo1 NNNCTGAGACATCCAACCAT NGG 2 0.1294 Tier II
16 NC_000078.6 12 37334292 + Agmo NNNCTGAAACATCCAAGCTT NGG 2 0.095 Tier II
17 NC_000069.6 3 157286564 + Negr1 NNNCTGAAACTTCCAAACAT NAG 2 0.0798 Tier II
18 NC_000086.7 X 147139043 - Htr2c NNNCTGAAACAGCCAAACAC NGG 2 0.0455 Tier II
19 NC_000076.6 10 27230403 + Lama2 NNNCTGAAACATCCAAACAT NGT 1 0.0161 Tier II
20 NC_000084.6 18 23592812 - Dtna NNNCTGAAACATCTAAACAT NGT 2 0.0118 Tier II
21 NC_000072.6 6 96336166 - Tafa1 NNNCTGAAACATCCAATCAT NTG 2 0.0052 Tier II
22 NC_000069.6 3 36029035 + Ccdc144b NNNATGAAACATCCACACAT NGG 2 0.0 Tier II
23 NC_000067.6 1 11819444 + A830018L16Rik NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
24 NC_000067.6 1 157236919 - Rasal2 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
25 NC_000068.7 2 110686627 - Ano3 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
26 NC_000069.6 3 3623029 - Hnf4g NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
27 NC_000069.6 3 159861613 - Wls NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
28 NC_000071.6 5 48758202 - Kcnip4 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
29 NC_000072.6 6 62248839 - Ccser1 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
30 NC_000073.6 7 120263984 + Abca14 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
31 NC_000074.6 8 54904864 + Gpm6a NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
32 NC_000075.6 9 19501997 + Olfr851 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
33 NC_000075.6 9 55873227 - Scaper NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
34 NC_000075.6 9 111242606 - Mlh1 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
35 NC_000076.6 10 45429759 + Lin28b NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
36 NC_000078.6 12 34358491 - Hdac9 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
37 NC_000078.6 12 78270962 - Gphn NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
38 NC_000078.6 12 101660423 + Tc2n NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
39 NC_000079.6 13 68621204 - Adcy2 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
40 NC_000082.6 16 31731337 + Dlg1 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
41 NC_000082.6 16 49129931 + Myh15 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
42 NC_000084.6 18 78298049 + Slc14a2 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
43 NC_000085.6 19 16298171 + Gnaq NNNCTGAAACATCCACACTT NGG 2 0.0 Tier II
44 NC_000070.6 4 144943707 + Vps13d NNNCTGAAACATCCATACTT NGG 2 0.0 Tier II
45 NC_000072.6 6 21525600 + Kcnd2 NNNCTGAAACATCCATACTT NGG 2 0.0 Tier II
46 NC_000076.6 10 32691395 - Nkain2 NNNCTGAAACATCCATACTT NGG 2 0.0 Tier II
47 NC_000084.6 18 53910733 + Csnk1g3 NNNCTGAAACATCCATACTT NGG 2 0.0 Tier II
48 NC_000076.6 10 34076796 + Trappc3l NNNCTGAAGCATCCACACAT NGG 2 0.0 Tier II
49 NC_000074.6 8 58717810 - Galntl6 NNNCTGAAGCATCCATACAT NGG 2 0.0 Tier II
50 NC_000071.6 5 90722544 - Gm36084 NNNCTGAAAAATTCAAACAT NGG 2 0.6067 Tier III
51 NC_000075.6 9 113451542 + Gm39438 NNNTTGAAACATCCAAACTT NGG 2 0.4308 Tier III
52 NC_000068.7 2 48139490 - Gm13481 NNNCTGAAGCATCCAAACTT NGG 2 0.359 Tier III
53 NC_000073.6 7 25961661 + Cyp2b28-ps NNNCTGAAGCATCCAAACTT NGG 2 0.359 Tier III
54 NC_000074.6 8 7457832 + Gm30954 NNNCTGAAGCATCCAAACTT NGG 2 0.359 Tier III
55 NC_000070.6 4 52048207 - C630028M04Rik NNNCTGAAACATCCAAATTT NGG 2 0.3462 Tier III
56 NC_000079.6 13 61855088 + Gm7240 NNNCTGAAACATCCAAATTT NGG 2 0.3462 Tier III
57 NC_000079.6 13 52123796 + 4921525O09Rik NNNCAGAAACCTCCAAACAT NGG 2 0.2 Tier III
58 NC_000067.6 1 51540262 + Gm17767 NNNCTGAAAAATCCTAACAT NGG 2 0.1733 Tier III
59 NC_000076.6 10 92262139 + Gm20757 NNNCTGAAACATCCAAGCTT NGG 2 0.095 Tier III
60 NC_000073.6 7 38499785 + Gm6819 NNNCTGAAACATCCAAACAT NAT 2 0.0 Tier III
61 NC_000070.6 4 50900746 + Gm42287 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier III
62 NC_000072.6 6 41399927 + Tcrb NNNCTGAAACATCCACACTT NGG 2 0.0 Tier III
63 NC_000072.6 6 60399415 - A530053G22Rik NNNCTGAAACATCCACACTT NGG 2 0.0 Tier III
64 NC_000080.6 14 90252089 - 4930474H20Rik NNNCTGAAACATCCACACTT NGG 2 0.0 Tier III
65 NC_000086.7 X 155763502 - Gm15155 NNNCTGAAACATCCACACTT NGG 2 0.0 Tier III
66 NC_000081.6 15 16602865 + Gm2611 NNNCTGAAACATCCATACAT NGG 1 0.0 Tier III
67 NC_000070.6 4 144943707 + Gm42344 NNNCTGAAACATCCATACTT NGG 2 0.0 Tier III
68 NC_000070.6 4 89025933 - Gm34568 NNNCTGAAGCATCCACACAT NGG 2 0.0 Tier III
69 NC_000072.6 6 68761614 - Igk NNNCTGAAGCATCCACACAT NGG 2 0.0 Tier III
70 NC_000080.6 14 109448721 + Gm30216 NNNCTGAAGCATCCACACAT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)