Construct: sgRNA BRDN0001148975

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAGTTTGATTATGTGAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIK2 (23235)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77139

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 111616338 - SIK2 NNNGTTTGATTATGTGAGGG NGG 0 1.0 Tier I
2 NC_000012.12 12 100880698 + ANO4 NNNATTTGATTATGTGAGGA NGG 2 0.8438 Tier II
3 NC_000004.12 4 70980560 + MOB1B NNNGTTTGATTAAGTAAGGG NGG 2 0.6923 Tier II
4 NC_000009.12 9 20207003 - SLC24A2 NNNGTTTAATCATGTGAGGG NGG 2 0.6667 Tier II
5 NC_000004.12 4 47910094 - NFXL1 NNNGTTTGTTTATGTGAGAG NGG 2 0.4286 Tier II
6 NC_000011.10 11 57234989 - APLNR NNNGTTTCATTATGTGAGTG NGG 2 0.4103 Tier II
7 NC_000017.11 17 18128973 - MYO15A NNNGTTTGATTATCTGAGGA NGG 2 0.4018 Tier II
8 NC_000015.10 15 65541549 + HACD3 NNNCTTTGATTATGTGATGG NGG 2 0.3529 Tier II
9 NC_000007.14 7 159060535 - VIPR2 NNNGTTAGATGATGTGAGGG NGG 2 0.35 Tier II
10 NC_000004.12 4 185759435 + SORBS2 NNNGTTTGTGTATGTGAGGG NGG 2 0.3 Tier II
11 NC_000010.11 10 86269980 - GRID1 NNNGTTTAATTCTGTGAGGG NGG 2 0.2632 Tier II
12 NC_000018.10 18 76873624 + ZNF236 NNNGTGTGATGATGTGAGGG NGG 2 0.2286 Tier II
13 NC_000023.11 X 110212056 - AMMECR1 NNNGTTTGATAATGTGAGGG NTG 2 0.0292 Tier II
14 NC_000009.12 9 96816947 + ZNF782 NNNGTTTTATTATGTGAGGG NTG 2 0.0244 Tier II
15 NC_000003.12 3 134248438 - RYK NNNGTTTGATTATGTCATGG NGG 2 0.0 Tier II
16 NC_000012.12 12 119265053 - LOC124903032 NNNGTTAGATTATGTGAGTG NGG 2 0.5833 Tier III
17 NC_000005.10 5 102694150 - LOC105379104 NNNGTGTGAATATGTGAGGG NGG 2 0.4898 Tier III
18 NC_000006.12 6 44772989 - LOC101929770 NNNCTTTGATTATGTGATGG NGG 2 0.3529 Tier III
19 NC_000013.11 13 41140791 + KBTBD6-DT NNNGTTTGATTATCTGAGTG NGG 2 0.2857 Tier III
20 NC_000023.11 X 128581379 + LOC107985698 NNNGTTGGATTTTGTGAGGG NGG 2 0.1961 Tier III
21 NC_000004.12 4 58526906 + LINC02494 NNNGTATGATTATGTGAGGG NGT 2 0.014 Tier III
22 NC_000007.14 7 19831964 - LOC105379720 NNNGTTTGATTATGGGTGGG NGG 2 0.0067 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 5160730 - Prkaa1 NNNGTTTGATGATGTGAGGG NGC 2 0.0089 Tier I
2 NC_000083.6 17 24403264 - Abca3 NNNGTTTGTTTATGTGAGGG NGG 1 0.6 Tier II
3 NC_000068.7 2 14885472 + Cacnb2 NNNGTTTGAGTATGTAAGGG NGG 2 0.5 Tier II
4 NC_000086.7 X 56786953 + Fhl1 NNNGTTTGATCATATGAGGG NGG 2 0.5 Tier II
5 NC_000071.6 5 92363585 - Art3 NNNGTGTGGTTATGTGAGGG NGG 2 0.381 Tier II
6 NC_000073.6 7 80657603 - Crtc3 NNNTTTTAATTATGTGAGGG NGG 2 0.3636 Tier II
7 NC_000079.6 13 96593108 + Cert1 NNNTTTTAATTATGTGAGGG NGG 2 0.3636 Tier II
8 NC_000070.6 4 101261535 - Jak1 NNNGTGTGTTTATGTGAGGG NGG 2 0.3429 Tier II
9 NC_000069.6 3 138544607 + Eif4e NNNGTTTGTGTATGTGAGGG NGG 2 0.3 Tier II
10 NC_000072.6 6 61428576 + Ccser1 NNNGAGTGATTATGTGAGGG NGG 2 0.2857 Tier II
11 NC_000074.6 8 108712508 + Zfhx3 NNNGTTTCATTATGTGAGCG NGG 2 0.2759 Tier II
12 NC_000073.6 7 106630553 - Gm1966 NNNGTTTTATTATTTGAGGG NGG 2 0.1667 Tier II
13 NC_000072.6 6 87210457 + Antxr1 NNNGTTTGATCATGTGTGGG NGG 2 0.0889 Tier II
14 NC_000081.6 15 5160960 - Prkaa1 NNNGTTTGATCATGTGAGGG NGA 2 0.0463 Tier II
15 NC_000081.6 15 5160776 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
16 NC_000081.6 15 5160868 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
17 NC_000081.6 15 5160914 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
18 NC_000081.6 15 5161006 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
19 NC_000081.6 15 5161051 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
20 NC_000081.6 15 5161097 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
21 NC_000081.6 15 5161143 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
22 NC_000081.6 15 5161189 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
23 NC_000081.6 15 5161235 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
24 NC_000081.6 15 5161281 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
25 NC_000081.6 15 5161327 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
26 NC_000081.6 15 5161373 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
27 NC_000081.6 15 5161419 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
28 NC_000081.6 15 5161465 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
29 NC_000081.6 15 5161511 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
30 NC_000081.6 15 5161556 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
31 NC_000081.6 15 5161615 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
32 NC_000081.6 15 5161753 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
33 NC_000081.6 15 5161799 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
34 NC_000081.6 15 5161845 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
35 NC_000081.6 15 5161891 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
36 NC_000081.6 15 5161936 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
37 NC_000081.6 15 5161995 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
38 NC_000081.6 15 5162039 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
39 NC_000081.6 15 5162098 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
40 NC_000081.6 15 5162144 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
41 NC_000081.6 15 5162203 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
42 NC_000081.6 15 5162248 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
43 NC_000081.6 15 5162293 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
44 NC_000081.6 15 5162338 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
45 NC_000081.6 15 5162428 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
46 NC_000081.6 15 5162473 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
47 NC_000081.6 15 5162518 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
48 NC_000081.6 15 5162564 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
49 NC_000081.6 15 5162623 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
50 NC_000081.6 15 5162668 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
51 NC_000081.6 15 5162727 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
52 NC_000081.6 15 5162786 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
53 NC_000081.6 15 5162878 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
54 NC_000081.6 15 5162937 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
55 NC_000081.6 15 5162984 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
56 NC_000081.6 15 5163030 - Prkaa1 NNNGTTTGATGATGTGAGGG NGA 2 0.0278 Tier II
57 NC_000086.7 X 41567162 + Gria3 NNNGTTTTATTATGTGAGGG NTG 2 0.0244 Tier II
58 NC_000079.6 13 93681018 - Dmgdh NNNGTTTGTTTATGTGAGGG NGT 2 0.0097 Tier II
59 NC_000073.6 7 63149585 - Chrna7 NNNGTTTGATTATGGGGGGG NGG 2 0.0088 Tier II
60 NC_000075.6 9 76251810 + Hcrtr2 NNNGTTTGATTATGTGTGGG NGC 2 0.003 Tier II
61 NC_000073.6 7 80657603 - Gm51450 NNNTTTTAATTATGTGAGGG NGG 2 0.3636 Tier III
62 NC_000084.6 18 38029339 + Gm30093 NNNGTTTGCTTATGTGGGGG NGG 2 0.1008 Tier III
63 NC_000071.6 5 131393177 + Gm29681 NNNGTTTGATTATGTGTGAG NGG 2 0.0952 Tier III
64 NC_000080.6 14 41891783 - LOC115488376 NNNGTTTGATTATGGGAGGG NGG 1 0.05 Tier III
Download CSV

Other clones with same target sequence:

(none)