Construct: sgRNA BRDN0001149197

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTGCTGAAAGATTGCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIK3 (23387)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75757

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 116859562 + SIK3 NNNGCTGAAAGATTGCACTG NGG 0 1.0 Tier I
2 NC_000015.10 15 78985053 + RASGRF1 NNNGCCGGAAGATTGCACTG NGG 2 0.6667 Tier I
3 NC_000003.12 3 197880725 + LRCH3 NNNGCTGAAAGATTGCACTC NGG 1 0.4286 Tier I
4 NC_000007.14 7 21867512 + DNAH11 NNNGCTGGAAAATTGCACTG NGG 2 0.7333 Tier II
5 NC_000004.12 4 145494264 - SMAD1 NNNGCTGAAAGAATGAACTG NGG 2 0.6923 Tier II
6 NC_000002.12 2 105416539 + FHL2 NNNGCTTAAAGATTGCAATG NGG 2 0.3077 Tier II
7 NC_000009.12 9 8819868 + PTPRD NNNGCTGAAAGATCACACTG NGG 2 0.2689 Tier II
8 NC_000010.11 10 73118318 + NUDT13 NNNTCTGAAAGATAGCACTG NGG 2 0.2251 Tier II
9 NC_000010.11 10 125801253 - UROS NNNGCAGAAAGATTGCACTG NAG 2 0.2247 Tier II
10 NC_000011.10 11 101577148 - TRPC6 NNNGGTGAAAGATTGCAGTG NGG 2 0.08 Tier II
11 NC_000011.10 11 18485902 + TSG101 NNNGCTGAAAGATTGGACTG NTG 2 0.006 Tier II
12 NC_000010.11 10 85683502 + GRID1 NNNGCTGAAACATGGCACTG NGG 2 0.0 Tier II
13 NC_000015.10 15 78985053 + LOC100129540 NNNGCCGGAAGATTGCACTG NGG 2 0.6667 Tier III
14 NC_000007.14 7 46971161 + LOC105375267 NNNGCTGAAATATTACACTG NGG 2 0.362 Tier III
15 NC_000023.11 X 8201997 - LOC107985675 NNNGCTGAAACAATGCACTG NGG 2 0.2967 Tier III
16 NC_000006.12 6 2299144 - GMDS-DT NNNGCTGAAAGATTGGACTG NTG 2 0.006 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 8758308 - Adarb2 NNNACCGAAAGATTGCACTG NGG 2 0.8182 Tier II
2 NC_000078.6 12 117915994 - Dnah11 NNNGCTGGAAAATTGCACTG NGG 2 0.7333 Tier II
3 NC_000067.6 1 39392607 + Tbc1d8 NNNGCTGAATGACTGCACTG NGG 2 0.6966 Tier II
4 NC_000070.6 4 147912756 - Plod1 NNNGCTGAAAGATAGCACTA NGG 2 0.5804 Tier II
5 NC_000070.6 4 56763484 - Elp1 NNNGCTGAAAGACTGCACTT NGG 2 0.5526 Tier II
6 NC_000082.6 16 10362318 + Tekt5 NNNGGTGAAAGACTGCACTG NGG 2 0.4737 Tier II
7 NC_000072.6 6 97249166 - Lmod3 NNNGCTGAAAGTTTGAACTG NGG 2 0.3333 Tier II
8 NC_000074.6 8 80045954 - Hhip NNNGCTTAAAGATTGCACAG NGG 2 0.1633 Tier II
9 NC_000068.7 2 113184963 - Ryr3 NNNGCTGAAAGATTGCACAC NGG 2 0.1224 Tier II
10 NC_000077.6 11 99478905 - Krt23 NNNTCTGAAAGATTGCACAG NGG 2 0.1039 Tier II
11 NC_000071.6 5 81119250 + Adgrl3 NNNGCTGAAAGATTGCAGTT NGG 2 0.0933 Tier II
12 NC_000071.6 5 34617668 + Add1 NNNGCTGAAAGATTCCTCTG NGG 2 0.0364 Tier II
13 NC_000068.7 2 104592376 - Cstf3 NNNGCTGAAAGATTGCAATG NGT 2 0.0087 Tier II
14 NC_000073.6 7 44141080 + Klk1b16 NNNGCTGAAAGATGGAACTG NGG 2 0.0 Tier II
15 NC_000067.6 1 72292884 - Tmem169 NNNGCTGAAAGATGGCTCTG NGG 2 0.0 Tier II
16 NC_000082.6 16 11182808 - 4930509G22Rik NNNGCTGAAAGACTGGACTG NGG 2 0.1215 Tier III
17 NC_000068.7 2 102955735 + Gm34483 NNNGCTGAAAGATTCCACAG NGG 2 0.0779 Tier III
18 NC_000081.6 15 6040447 + Gm41259 NNNGCTGAAAGATTTCCCTG NGG 2 0.0252 Tier III
Download CSV

Other clones with same target sequence:

(none)