Construct: sgRNA BRDN0001149230

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCCTCCCCACTGAGGAACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA2 (6196)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75735

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 166488900 + RPS6KA2 NNNTCCCCACTGAGGAACTG NGG 0 1.0 Tier I
2 NC_000022.11 22 31815092 + DEPDC5 NNNTGCCCACTGAGGAAGTG NGG 2 0.08 Tier I
3 NC_000016.10 16 77209422 + SYCE1L NNNTCCCCACAGAGGCACTG NGG 2 0.0 Tier I
4 NC_000010.11 10 44996363 - LOC124902530 NNNTCACCACTGAGGAACTG NGG 1 0.9286 Tier II
5 NC_000022.11 22 41250807 - RANGAP1 NNNTCACCACTGAGAAACTG NGG 2 0.8739 Tier II
6 NC_000022.11 22 37812699 - GCAT NNNTTCCCACTGAGGAACTG NGG 1 0.6364 Tier II
7 NC_000003.12 3 128283847 + EEFSEC NNNTCCACACTGGGGAACTG NGG 2 0.4891 Tier II
8 NC_000005.10 5 1109416 + SLC12A7 NNNACCCCACTGAGAAACTG NGG 2 0.4482 Tier II
9 NC_000016.10 16 83648995 + CDH13 NNNTTCCCACTGGGGAACTG NGG 2 0.415 Tier II
10 NC_000023.11 X 108407315 + COL4A6 NNNTTCCAACTGAGGAACTG NGG 2 0.4136 Tier II
11 NC_000016.10 16 2758705 - SRRM2 NNNTCCCCACTGAGAAACTC NGG 2 0.4034 Tier II
12 NC_000008.11 8 19603221 - CSGALNACT1 NNNTCCCCATGGAGGAACTG NGG 2 0.3765 Tier II
13 NC_000001.11 1 6125634 - CHD5 NNNTCCCACCTGAGGAACTG NGG 2 0.3714 Tier II
14 NC_000005.10 5 75140784 + ANKRD31 NNNTCACCAGTGAGGAACTG NGG 2 0.3611 Tier II
15 NC_000009.12 9 113564601 - RGS3 NNNTCCCCAAGGAGGAACTG NGG 2 0.3467 Tier II
16 NC_000008.11 8 14187125 + SGCZ NNNTACCCTCTGAGGAACTG NGG 2 0.3429 Tier II
17 NC_000014.9 14 81506983 + SEL1L NNNTCCCCTCTCAGGAACTG NGG 2 0.3176 Tier II
18 NC_000010.11 10 32713393 - CCDC7 NNNTCCCCAGTGGGGAACTG NGG 2 0.2536 Tier II
19 NC_000001.11 1 230172305 + GALNT2 NNNGCCCCAGTGAGGAACTG NGG 2 0.2431 Tier II
20 NC_000020.11 20 37353817 - SRC NNNTGCCCAGTGAGGAACTG NGG 2 0.2333 Tier II
21 NC_000003.12 3 38564943 - SCN5A NNNTCCCCATTGAGGGACTG NGG 2 0.181 Tier II
22 NC_000009.12 9 28589 - WASHC1 NNNTACCCACTGTGGAACTG NGG 2 0.1714 Tier II
23 NC_000017.11 17 32479984 - PSMD11 NNNTCCCCACTGAGAAGCTG NGG 2 0.1661 Tier II
24 NC_000007.14 7 139974894 - TBXAS1 NNNTCCCCACTGATGAAATG NGG 2 0.1436 Tier II
25 NC_000004.12 4 71320943 + SLC4A4 NNNTCGCCACTGATGAACTG NGG 2 0.1333 Tier II
26 NC_000006.12 6 52549324 - TRAM2 NNNTCCCCACTGATGAACTC NGG 2 0.1143 Tier II
27 NC_000007.14 7 2009974 + MAD1L1 NNNTTCCCACTGAGGACCTG NGG 2 0.1123 Tier II
28 NC_000016.10 16 11088119 - CLEC16A NNNTCCGCACTGAGGGACTG NGG 2 0.0905 Tier II
29 NC_000002.12 2 109515547 + RANBP2 NNNTCCCCACTGAGGACCTC NGG 2 0.0756 Tier II
30 NC_000010.11 10 46303836 + ANTXRL NNNTCCCCACTGAGGGCCTG NGG 2 0.0339 Tier II
31 NC_000001.11 1 63786047 + ROR1 NNNTCCCCACTGAGGAAGGG NGG 2 0.0333 Tier II
32 NC_000009.12 9 76412720 - GCNT1 NNNACCCCACTGAGGAACTG NGA 2 0.0331 Tier II
33 NC_000002.12 2 138694240 + NXPH2 NNNTCCCAACTGAGGAACTG NTG 2 0.0253 Tier II
34 NC_000001.11 1 183135682 - LAMC1 NNNTCCCCAGTGAGGAACTG NTG 2 0.0152 Tier II
35 NC_000020.11 20 47708654 + SULF2 NNNTCCCCACTGGGGAACTG NGT 2 0.0105 Tier II
36 NC_000002.12 2 36490865 + CRIM1 NNNTCCCCACTGAGTAACTG NGC 2 0.0032 Tier II
37 NC_000018.10 18 48202625 - ZBTB7C NNNTCCCCACTGAGGATCTG NGC 2 0.003 Tier II
38 NC_000017.11 17 1478219 - MYO1C NNNTCCCCACTGAGCCACTG NGG 2 0.0 Tier II
39 NC_000007.14 7 4720544 - FOXK1 NNNTCCCCACTGAGGCACAG NGG 2 0.0 Tier II
40 NC_000008.11 8 514691 - TDRP NNNTCCCCACTGAGGCACTG NGG 1 0.0 Tier II
41 NC_000019.10 19 47071091 + ZC3H4 NNNTCCCCACTGAGGCACTG NGC 2 0.0 Tier II
42 NC_000010.11 10 44996363 - ZNF22-AS1 NNNTCACCACTGAGGAACTG NGG 1 0.9286 Tier III
43 NC_000005.10 5 133117476 - LOC105379178 NNNTGCCCATTGAGGAACTG NGG 2 0.5647 Tier III
44 NC_000012.12 12 92275967 + LINC02391 NNNACACCACTGAGGAACTG NGG 2 0.4422 Tier III
45 NC_000005.10 5 17153711 + BASP1-AS1 NNNTCCCCACTGGGGAACTC NGG 2 0.2795 Tier III
46 NC_000003.12 3 126208894 - ALDH1L1-AS2 NNNTCCCCTCTTAGGAACTG NGG 2 0.2308 Tier III
47 NC_000023.11 X 8452048 - LOC107985675 NNNTCCACACTGTGGAACTG NGG 2 0.225 Tier III
48 NC_000001.11 1 28476 - WASH7P NNNTACCCACTGTGGAACTG NGG 2 0.1714 Tier III
49 NC_000001.11 1 198994 - WASH9P NNNTACCCACTGTGGAACTG NGG 2 0.1714 Tier III
50 NC_000002.12 2 113584979 + WASH2P NNNTACCCACTGTGGAACTG NGG 2 0.1714 Tier III
51 NC_000012.12 12 30532 - WASH8P NNNTACCCACTGTGGAACTG NGG 2 0.1714 Tier III
52 NC_000015.10 15 101962485 + WASH3P NNNTACCCACTGTGGAACTG NGG 2 0.1714 Tier III
53 NC_000019.10 19 70084 - WASH5P NNNTACCCACTGTGGAACTG NGG 2 0.1714 Tier III
54 NC_000013.11 13 100738696 + NALCN-AS1 NNNTCACCACTGAGGAGCTG NGG 2 0.1639 Tier III
55 NC_000017.11 17 83177922 - LOC101929650 NNNTCCCCACAGAGGAGCTG NGG 2 0.1324 Tier III
56 NC_000007.14 7 20972419 + LINC01162 NNNTCCCCACTGAAGAGCTG NGG 2 0.1324 Tier III
57 NC_000012.12 12 53817127 - LOC107984525 NNNACCCCACTGAGGAGCTG NGG 2 0.084 Tier III
58 NC_000012.12 12 74274689 - LINC02882 NNNTCCCCACTGAGGAACTG NTG 1 0.039 Tier III
59 NC_000010.11 10 46262492 - ANTXRLP1 NNNTCCCCACTGAGGGCCTG NGG 2 0.0339 Tier III
60 NC_000021.9 21 42018560 + ZNF295-AS1 NNNTCCCCACTGTGGAACTG NGA 2 0.0208 Tier III
61 NC_000001.11 1 221855028 - LOC124904517 NNNTCCCCACTGAGGAACTT NGT 2 0.0113 Tier III
62 NC_000006.12 6 76538198 - LINC02540 NNNTCCCCACTGTGGAACTG NGT 2 0.0048 Tier III
63 NC_000008.11 8 105918775 + ZFPM2-AS1 NNNTCCCCACTGAGGAACTG NCT 2 0.0 Tier III
64 NC_000014.9 14 44039130 + LINC02307 NNNTCCCCACTGAGGAACTG NCT 2 0.0 Tier III
65 NC_000016.10 16 56989236 + LOC124903696 NNNTCCCCACTGAGGCACTG NGA 2 0.0 Tier III
66 NC_000020.11 20 5492194 + LOC124904863 NNNTCCCCACTGATGCACTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 7262150 - Rps6ka2 NNNTCCGCACTGAGGAACTG NGG 1 0.4706 Tier I
2 NC_000068.7 2 91933851 + Dgkz NNNTCCCCACTGGGGAACAG NGG 2 0.1863 Tier I
3 NC_000071.6 5 32918025 + Depdc5 NNNTGCCCACTGAGGAAGTG NGG 2 0.08 Tier I
4 NC_000078.6 12 108870881 - Wars NNNTCCCCACTGGGAAACTG NGG 2 0.6138 Tier II
5 NC_000073.6 7 107403903 - Syt9 NNNGCTCCACTGAGGAACTG NGG 2 0.5804 Tier II
6 NC_000070.6 4 31970412 + Map3k7 NNNTCTCCACTGAGGAAATG NGG 2 0.5 Tier II
7 NC_000083.6 17 56107886 + Plin4 NNNTCCCAACAGAGGAACTG NGG 2 0.4875 Tier II
8 NC_000085.6 19 24697943 + Pgm5 NNNTCCTCCCTGAGGAACTG NGG 2 0.4643 Tier II
9 NC_000080.6 14 54647294 - Acin1 NNNTGCCCACTGAGGAACTT NGG 2 0.42 Tier II
10 NC_000077.6 11 80534857 - Myo1d NNNTCCACACTCAGGAACTG NGG 2 0.3971 Tier II
11 NC_000073.6 7 55928097 + Cyfip1 NNNTCCCATCTGAGGAACTG NGG 2 0.39 Tier II
12 NC_000073.6 7 144716974 - Ano1 NNNTCTCCACTGAGGAACAG NGG 2 0.2653 Tier II
13 NC_000076.6 10 79873046 + Plppr3 NNNCCCCCAGTGAGGAACTG NGG 2 0.2516 Tier II
14 NC_000075.6 9 26976652 + Acad8 NNNTCCCCACTCAGGAACTC NGG 2 0.2269 Tier II
15 NC_000082.6 16 90111776 + Tiam1 NNNTCCCCACTGTAGAACTG NGG 2 0.225 Tier II
16 NC_000071.6 5 112971388 + Grk3 NNNTCCACACTGAGGAACAG NGG 2 0.2143 Tier II
17 NC_000085.6 19 58954508 + Eno4 NNNTCTCCACTGAGGACCTG NGG 2 0.1639 Tier II
18 NC_000068.7 2 32037029 - Nup214 NNNTCCCCACTGAGGAAATG NAG 2 0.1396 Tier II
19 NC_000083.6 17 48415481 - Oard1 NNNTCCCCACAGAGGAGCTG NGG 2 0.1324 Tier II
20 NC_000073.6 7 19867430 + Ceacam16 NNNCCCCCACTGAGGAGCTG NGG 2 0.1142 Tier II
21 NC_000079.6 13 36944162 + F13a1 NNNTCCACACTGAGGAAGTG NGG 2 0.1 Tier II
22 NC_000073.6 7 37411204 + Zfp536 NNNTCCCCAGTGAGGGACTG NGG 2 0.0748 Tier II
23 NC_000073.6 7 43848204 + Klk5 NNNTCCCCAGTGAGGGACTG NGG 2 0.0748 Tier II
24 NC_000071.6 5 128822525 - Rimbp2 NNNTCCCCAGTGAGGAGCTG NGG 2 0.0686 Tier II
25 NC_000071.6 5 135469242 + Hip1 NNNTCCCCAGTGAGGAGCTG NGG 2 0.0686 Tier II
26 NC_000071.6 5 135469506 + Hip1 NNNTCCCCAGTGAGGAGCTG NGG 2 0.0686 Tier II
27 NC_000073.6 7 19789498 - Cblc NNNTCCCCAGTGAGGAGCTG NGG 2 0.0686 Tier II
28 NC_000083.6 17 90169904 - Nrxn1 NNNTCCCCACTGGGGAACTG NGA 2 0.0453 Tier II
29 NC_000083.6 17 63203938 + Fbxl17 NNNGCCCCACTGAGGAACTG NTG 2 0.0244 Tier II
30 NC_000084.6 18 75389426 - Smad7 NNNTCCCCACTGAGAAACTG NGT 2 0.0152 Tier II
31 NC_000074.6 8 13074230 - Proz NNNTCCCCACTGAGGAGCTG NTG 2 0.0069 Tier II
32 NC_000085.6 19 4590751 - Pcx NNNTCCCCACTGTGGAACTG NGC 2 0.0067 Tier II
33 NC_000083.6 17 56810983 + Rfx2 NNNTCCCCACTGTGGAACTG NGT 2 0.0048 Tier II
34 NC_000082.6 16 41942910 - Lsamp NNNTCCCCACAGAGGTACTG NGG 2 0.0 Tier II
35 NC_000073.6 7 142734241 - Gm39116 NNNTCCCCACTGAGAAACTG NGG 1 0.9412 Tier III
36 NC_000067.6 1 120434900 + Gm46154 NNNACTCCACTGAGGAACTG NGG 2 0.4422 Tier III
37 NC_000073.6 7 142846658 - Gm6471 NNNTCCCCAGTGAGAAACTG NGG 2 0.366 Tier III
38 NC_000073.6 7 142846658 - Gm39117 NNNTCCCCAGTGAGAAACTG NGG 2 0.366 Tier III
39 NC_000083.6 17 36760812 - Gm8878 NNNTCCCCATTGAGGAACTG NAG 2 0.244 Tier III
40 NC_000068.7 2 91933851 + Gm39876 NNNTCCCCACTGGGGAACAG NGG 2 0.1863 Tier III
41 NC_000078.6 12 90755177 + Gm26512 NNNTCCCCACTGAGGATCTA NGG 2 0.125 Tier III
42 NC_000068.7 2 113970428 - Gm39909 NNNCCCCCACTGAGGACCTG NGG 2 0.1142 Tier III
43 NC_000084.6 18 61472930 + Lncbate10 NNNTCCCCAGTGAGCAACTG NGG 2 0.1061 Tier III
44 NC_000079.6 13 34132383 + Gm36500 NNNTCCCCCCTGAGGAGCTG NGG 2 0.1008 Tier III
45 NC_000079.6 13 36944162 + Gm30489 NNNTCCACACTGAGGAAGTG NGG 2 0.1 Tier III
46 NC_000072.6 6 34212354 - Gm32533 NNNTTCCCACTGAGGATCTG NGG 2 0.0848 Tier III
47 NC_000073.6 7 19838614 + Gm34891 NNNTCCCCAGTGAGGGACTG NGG 2 0.0748 Tier III
48 NC_000070.6 4 131156560 + Gm42318 NNNTCCCCACTGAGGATATG NGG 2 0.0718 Tier III
49 NC_000081.6 15 36956141 - Gm34590 NNNTCCCCAGTGAGGACCTG NGG 2 0.0686 Tier III
50 NC_000071.6 5 128822525 - Gm34057 NNNTCCCCAGTGAGGAGCTG NGG 2 0.0686 Tier III
51 NC_000073.6 7 19789498 - LOC115486450 NNNTCCCCAGTGAGGAGCTG NGG 2 0.0686 Tier III
52 NC_000085.6 19 59537611 - 2700089I24Rik NNNTCCCCAGTGAGGATCTG NGG 2 0.0519 Tier III
53 NC_000078.6 12 79875221 - Gm46361 NNNTCTCCACTGAGGAACTG NGT 2 0.015 Tier III
54 NC_000078.6 12 114464017 + Igh NNNTCCCAACTGAGGAACTG NGC 2 0.0144 Tier III
55 NC_000079.6 13 107032901 - 3830408C21Rik NNNTCCCCACTGAGGCACTG NTG 2 0.0 Tier III
56 NC_000076.6 10 13077052 + Gm40602 NNNTCCCCACTGATGCACTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)