Construct: sgRNA BRDN0001149311

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCTCCTCAGTACCACAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGKZ (8525)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77465

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 46371494 + DGKZ NNNTCCTCAGTACCACAGCA NGG 0 1.0 Tier I
2 NC_000002.12 2 130172838 - SMPD4 NNNTCCTCTGTACCACAACA NGG 2 0.4154 Tier I
3 NC_000017.11 17 43825661 + MPP3 NNNTCCTCAGGACAACAGCA NGG 2 0.14 Tier I
4 NC_000011.10 11 132328175 + NTM NNNTCCTTAGTACCAAAGCA NGG 2 0.875 Tier II
5 NC_000002.12 2 98557488 + INPP4A NNNTCCACAGTGCCACAGCA NGG 2 0.6319 Tier II
6 NC_000002.12 2 215388646 - FN1 NNNTCCACAGCACCACAGCA NGG 2 0.5833 Tier II
7 NC_000008.11 8 133094966 - SLA NNNTCCTCAGTGCCACATCA NGG 2 0.4815 Tier II
8 NC_000008.11 8 133094966 - TG NNNTCCTCAGTGCCACATCA NGG 2 0.4815 Tier II
9 NC_000005.10 5 11868185 - CTNND2 NNNTCCTCAGCACCACAGCT NGG 2 0.4 Tier II
10 NC_000011.10 11 123396066 - GRAMD1B NNNTCCTCTGTACCACAGCT NGG 2 0.36 Tier II
11 NC_000004.12 4 6709658 + MRFAP1L1 NNNTCCTCAGTACCGCAGTA NGG 2 0.3 Tier II
12 NC_000023.11 X 154541699 + G6PD NNNTCCTGAGGACCACAGCA NGG 2 0.2571 Tier II
13 NC_000023.11 X 154541699 + IKBKG NNNTCCTGAGGACCACAGCA NGG 2 0.2571 Tier II
14 NC_000013.11 13 98951520 + DOCK9 NNNTTCTCAGGACCACAGCA NGG 2 0.2545 Tier II
15 NC_000012.12 12 41295047 + PDZRN4 NNNTCATCAGTACCACAGCA NAG 2 0.2407 Tier II
16 NC_000006.12 6 33674567 + ITPR3 NNNTCCCCAGTCCCACAGCA NGG 2 0.1809 Tier II
17 NC_000017.11 17 8514540 + MYH10 NNNTCCTCAGCCCCACAGCA NGG 2 0.1754 Tier II
18 NC_000006.12 6 34122698 + GRM4 NNNTCCTCAGTTCCACACCA NGG 2 0.1587 Tier II
19 NC_000019.10 19 38083194 - SIPA1L3 NNNTCCTCAGTGCCAGAGCA NGG 2 0.1111 Tier II
20 NC_000004.12 4 76277118 - FAM47E NNNTCCTCAGTAGCACATCA NGG 2 0.0909 Tier II
21 NC_000004.12 4 76277118 - FAM47E-STBD1 NNNTCCTCAGTAGCACATCA NGG 2 0.0909 Tier II
22 NC_000014.9 14 64090037 - SYNE2 NNNTCCTCAGTACCAGTGCA NGG 2 0.0205 Tier II
23 NC_000002.12 2 89600061 + IGK NNNTCCACAGTGCCACAGCA NGG 2 0.6319 Tier III
24 NC_000008.11 8 101262891 + LINC02844 NNNTGCTCAGTGCCACAGCA NGG 2 0.4333 Tier III
25 NC_000008.11 8 101262891 + LOC107984005 NNNTGCTCAGTGCCACAGCA NGG 2 0.4333 Tier III
26 NC_000002.12 2 131501020 + SMPD4BP NNNTCCTCTGTACCACAACA NGG 2 0.4154 Tier III
27 NC_000008.11 8 95668253 - CFAP418-AS1 NNNTCCTCAGTACCAAAGCC NGG 2 0.2273 Tier III
28 NC_000006.12 6 139976392 - LINC02941 NNNTCCTCAGTACCTGAGCA NGG 2 0.0308 Tier III
29 NC_000007.14 7 94310067 + LOC112267858 NNNTCCTCAGTACCACAGAA NGT 2 0.0069 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 3476010 + Tex45 NNNTCGTCAGCACCACAGCA NGG 2 0.3333 Tier I
2 NC_000083.6 17 24044376 + Prss27 NNNTCCTCAGTACCAAGGCA NGG 2 0.1765 Tier I
3 NC_000078.6 12 17293165 - Atp6v1c2 NNNTCCTCATTACCACAGCA NGG 1 0.8125 Tier II
4 NC_000067.6 1 6751992 - St18 NNNTCTTTAGTACCACAGCA NGG 2 0.8125 Tier II
5 NC_000067.6 1 74364453 + Catip NNNTCCTCAGAACCACAGCA NGG 1 0.75 Tier II
6 NC_000072.6 6 83281774 - Slc4a5 NNNTCCCCAGTACCACAGCA NGG 1 0.6875 Tier II
7 NC_000071.6 5 108674805 - Idua NNNTTTTCAGTACCACAGCA NGG 2 0.5909 Tier II
8 NC_000071.6 5 108674805 - Slc26a1 NNNTTTTCAGTACCACAGCA NGG 2 0.5909 Tier II
9 NC_000076.6 10 74984474 + Gnaz NNNTCCTCGTTACCACAGCA NGG 2 0.5417 Tier II
10 NC_000076.6 10 74984474 + Rsph14 NNNTCCTCGTTACCACAGCA NGG 2 0.5417 Tier II
11 NC_000078.6 12 36097199 - Bzw2 NNNTCCTCAGAACCACATCA NGG 2 0.5 Tier II
12 NC_000073.6 7 115854623 + Sox6 NNNTCTTCACTACCACAGCA NGG 2 0.3714 Tier II
13 NC_000086.7 X 145297684 + Lhfpl1 NNNTCCTCATTACAACAGCA NGG 2 0.2844 Tier II
14 NC_000074.6 8 84675476 - Nacc1 NNNTTCTCAGTACCACAGAA NGG 2 0.2727 Tier II
15 NC_000081.6 15 66500152 - Lrrc6 NNNTCCTCATTTCCACAGCA NGG 2 0.2708 Tier II
16 NC_000068.7 2 130979250 + Atrn NNNTCCTCAGAACAACAGCA NGG 2 0.2625 Tier II
17 NC_000072.6 6 115251614 - Syn2 NNNTCCTGAGGACCACAGCA NGG 2 0.2571 Tier II
18 NC_000072.6 6 115251614 - Timp4 NNNTCCTGAGGACCACAGCA NGG 2 0.2571 Tier II
19 NC_000085.6 19 6468838 - Nrxn2 NNNTCCTGAGGACCACAGCA NGG 2 0.2571 Tier II
20 NC_000085.6 19 43699930 - Entpd7 NNNTCCTGAGGACCACAGCA NGG 2 0.2571 Tier II
21 NC_000084.6 18 5015879 - Svil NNNTCCTCAGCAACACAGCA NGG 2 0.2564 Tier II
22 NC_000070.6 4 101180886 - Jak1 NNNTCCGCAGTACCACAGAA NGG 2 0.2521 Tier II
23 NC_000071.6 5 137164564 - Muc3 NNNGCCTCAGGACCACAGCA NGG 2 0.25 Tier II
24 NC_000071.6 5 54062325 + Stim2 NNNTGCTCAGGACCACAGCA NGG 2 0.24 Tier II
25 NC_000070.6 4 46727494 + Gabbr2 NNNTCCTCAGTTCCACAGCT NGG 2 0.2 Tier II
26 NC_000072.6 6 114399811 + Hrh1 NNNTCCTCAGTACCAAGGCA NGG 2 0.1765 Tier II
27 NC_000073.6 7 99579295 - Arrb1 NNNTCCTCAGTACCACATCA NAG 2 0.1728 Tier II
28 NC_000080.6 14 33633979 - Ptpn20 NNNTCCTCAGTACTACAGCC NGG 2 0.1667 Tier II
29 NC_000079.6 13 43632920 - Rnf182 NNNTCCTCTGTCCCACAGCA NGG 2 0.1579 Tier II
30 NC_000067.6 1 134389758 - Mgat4f NNNTCCTCAGTACTTCAGCA NGG 2 0.1467 Tier II
31 NC_000074.6 8 115141024 - Wwox NNNTCCTGAGTACCACAGCC NGG 2 0.1461 Tier II
32 NC_000069.6 3 144535501 - Hs2st1 NNNTCCTCAGTATCTCAGCA NGG 2 0.14 Tier II
33 NC_000068.7 2 132847743 + Crls1 NNNTCCTCAGTTACACAGCA NGG 2 0.1282 Tier II
34 NC_000073.6 7 125846789 + D430042O09Rik NNNTCCTCAGTACCACAGAA NAG 2 0.1111 Tier II
35 NC_000083.6 17 66065133 + Ankrd12 NNNTCCTCAGTACCACAGAA NAG 2 0.1111 Tier II
36 NC_000068.7 2 5327743 + Camk1d NNNTCCTCAGTATCAGAGCA NGG 2 0.1077 Tier II
37 NC_000071.6 5 114394793 + Ube3b NNNTCCTCAGTCACACAGCA NGG 2 0.1012 Tier II
38 NC_000079.6 13 91520591 - Ssbp2 NNNTCCTCAGAACCACAGGA NGG 2 0.0938 Tier II
39 NC_000067.6 1 40232929 + Il1r1 NNNTCCTCAGCACCACTGCA NGG 2 0.0889 Tier II
40 NC_000067.6 1 83260143 - Sphkap NNNTCCTGAGTACCACAGGA NGG 2 0.0804 Tier II
41 NC_000072.6 6 73138817 - Dnah6 NNNTCCTCAGTCCCACAGCA NAG 2 0.0682 Tier II
42 NC_000069.6 3 105641795 + Kcnd3 NNNTCCTCAGGACCACAGGA NGG 2 0.05 Tier II
43 NC_000084.6 18 37638926 + Gm37013 NNNCCCTCAGTACCACAGCA NGT 2 0.0104 Tier II
44 NC_000084.6 18 37638926 + Gm38666 NNNCCCTCAGTACCACAGCA NGT 2 0.0104 Tier II
45 NC_000084.6 18 37638926 + Gm38667 NNNCCCTCAGTACCACAGCA NGT 2 0.0104 Tier II
46 NC_000078.6 12 34863538 - Hdac9 NNNTCCTCAGGACCACAGCA NGT 2 0.0065 Tier II
47 NC_000076.6 10 73976889 + Pcdh15 NNNTCCTCAGTACCACAGCA NAC 2 0.0 Tier II
48 NC_000077.6 11 32135581 + Gm39676 NNNTCCTCAGAACCACACCA NGG 2 0.3571 Tier III
49 NC_000071.6 5 137164564 - Gm40348 NNNGCCTCAGGACCACAGCA NGG 2 0.25 Tier III
50 NC_000082.6 16 55437615 - Gm45968 NNNTCCTCAGTACCATACCA NGG 2 0.1465 Tier III
51 NC_000076.6 10 76985352 + Gm35721 NNNTCCTCAGTATCACAGGA NGG 2 0.0875 Tier III
52 NC_000083.6 17 84141527 - Gm19696 NNNTCCTCAGTACCCCAGCC NGG 2 0.062 Tier III
53 NC_000077.6 11 56019880 + LOC115485826 NNNTCCTCAGAACCACAGCA NTG 2 0.0292 Tier III
54 NC_000080.6 14 63368156 - Gm36165 NNNTCCTCGGTACCACAGCA NGC 2 0.0148 Tier III
55 NC_000080.6 14 63368156 - Gm46458 NNNTCCTCGGTACCACAGCA NGC 2 0.0148 Tier III
56 NC_000085.6 19 55667364 - 4930552P12Rik NNNTCCTCAGTACCACAGAA NGT 2 0.0069 Tier III
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Other clones with same target sequence:

(none)