Construct: sgRNA BRDN0001149398
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTAACGGACTGCAGAACGGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80218
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
31685692 |
- |
OSBPL10 |
NNNAGGGACTGCAGAAAGGA |
NGG |
2 |
0.28 |
Tier II |
| 2 |
NC_000003.12 |
3 |
48553915 |
- |
PFKFB4 |
NNNAAGGACTGCAGAAAGGA |
NGG |
2 |
0.2667 |
Tier II |
| 3 |
NC_000020.11 |
20 |
25458954 |
+ |
NINL |
NNNACGGACTGCTGAAGGGA |
NGG |
2 |
0.0176 |
Tier II |
| 4 |
NC_000014.9 |
14 |
71070512 |
+ |
PCNX1 |
NNNACAGACTGCAGAACGGA |
NGT |
2 |
0.0161 |
Tier II |
| 5 |
NC_000017.11 |
17 |
82950704 |
+ |
B3GNTL1 |
NNNACGGTCTGCAGAACGGA |
NGC |
2 |
0.0095 |
Tier II |
| 6 |
NC_000007.14 |
7 |
8042196 |
- |
GLCCI1 |
NNNACGGACTGCAGAATGGA |
NGT |
2 |
0.0075 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
12375650 |
+ |
Pdzd2 |
NNNACGGAATCCAGAACGGA |
NGG |
2 |
0.3673 |
Tier I |
| 2 |
NC_000081.6 |
15 |
59532140 |
- |
Nsmce2 |
NNNAGGGACTGCAGGACGGA |
NGG |
2 |
0.39 |
Tier II |
| 3 |
NC_000074.6 |
8 |
108888053 |
- |
Zfhx3 |
NNNTCGGACTGGAGAACGGA |
NGG |
2 |
0.2828 |
Tier II |
| 4 |
NC_000075.6 |
9 |
92250347 |
+ |
Plscr1 |
NNNACGGACTTCAGAACAGA |
NGG |
2 |
0.2663 |
Tier II |
| 5 |
NC_000067.6 |
1 |
89931103 |
- |
Gbx2 |
NNNACGGACTGCAGGACGGA |
NAG |
2 |
0.1685 |
Tier II |
| 6 |
NC_000072.6 |
6 |
144380340 |
- |
Sox5 |
NNNACGGACTGCCGAACGGA |
NGC |
2 |
0.0047 |
Tier II |
| 7 |
NC_000067.6 |
1 |
156431382 |
+ |
Soat1 |
NNNACGGACTGCAGACCGAA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000079.6 |
13 |
120278340 |
+ |
Gm39470 |
NNNAGGGACTGCAGAAAGGA |
NGG |
2 |
0.28 |
Tier III |
Other clones with same target sequence:
(none)
