Construct: sgRNA BRDN0001149398

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTAACGGACTGCAGAACGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80218

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 31685692 - OSBPL10 NNNAGGGACTGCAGAAAGGA NGG 2 0.28 Tier II
2 NC_000003.12 3 48553915 - PFKFB4 NNNAAGGACTGCAGAAAGGA NGG 2 0.2667 Tier II
3 NC_000020.11 20 25458954 + NINL NNNACGGACTGCTGAAGGGA NGG 2 0.0176 Tier II
4 NC_000014.9 14 71070512 + PCNX1 NNNACAGACTGCAGAACGGA NGT 2 0.0161 Tier II
5 NC_000017.11 17 82950704 + QTGAL NNNACGGTCTGCAGAACGGA NGC 2 0.0095 Tier II
6 NC_000007.14 7 8042196 - GLCCI1 NNNACGGACTGCAGAATGGA NGT 2 0.0075 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 12375650 + Pdzd2 NNNACGGAATCCAGAACGGA NGG 2 0.3673 Tier I
2 NC_000081.6 15 59532140 - Nsmce2 NNNAGGGACTGCAGGACGGA NGG 2 0.39 Tier II
3 NC_000074.6 8 108888053 - Zfhx3 NNNTCGGACTGGAGAACGGA NGG 2 0.2828 Tier II
4 NC_000075.6 9 92250347 + Plscr1 NNNACGGACTTCAGAACAGA NGG 2 0.2663 Tier II
5 NC_000067.6 1 89931103 - Gbx2 NNNACGGACTGCAGGACGGA NAG 2 0.1685 Tier II
6 NC_000072.6 6 144380340 - Sox5 NNNACGGACTGCCGAACGGA NGC 2 0.0047 Tier II
7 NC_000067.6 1 156431382 + Soat1 NNNACGGACTGCAGACCGAA NGG 2 0.0 Tier II
8 NC_000079.6 13 120278340 + Gm39470 NNNAGGGACTGCAGAAAGGA NGG 2 0.28 Tier III
Download CSV

Other clones with same target sequence:

(none)

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