Construct: sgRNA BRDN0001149464

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTCACCACATAATTACCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGFR (1956)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75691

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 55155830 - EGFR NNNCACCACATAATTACCTG NGG 0 1.0 Tier I
2 NC_000012.12 12 70294295 - CNOT2 NNNCACCACATACTTACCTG NGC 2 0.0047 Tier I
3 NC_000010.11 10 73507216 + USP54 NNNCACTAGATAATTACCTG NGG 2 0.503 Tier II
4 NC_000005.10 5 14467423 - TRIO NNNCACCACACGATTACCTG NGG 2 0.4815 Tier II
5 NC_000016.10 16 48570125 + N4BP1 NNNCACCACATGATTACTTG NGG 2 0.4643 Tier II
6 NC_000018.10 18 52849291 - DCC NNNCAACACATAATTAACTG NGG 2 0.4333 Tier II
7 NC_000005.10 5 132750679 + CCNI2 NNNCACTACATAACTACCTG NGG 2 0.2321 Tier II
8 NC_000013.11 13 97149969 + MBNL2 NNNCACCACAAAATCACCTG NGG 2 0.2045 Tier II
9 NC_000007.14 7 23767784 - STK31 NNNCAGCACAGAATTACCTG NGG 2 0.2 Tier II
10 NC_000022.11 22 44493953 + RTL6 NNNCATCACATACTTACCTG NGG 2 0.1955 Tier II
11 NC_000012.12 12 5486870 - NTF3 NNNCACCACGTAACTACCTG NGG 2 0.1587 Tier II
12 NC_000011.10 11 20954577 - NELL1 NNNCACCACATAATTATCTG NAG 2 0.121 Tier II
13 NC_000019.10 19 33437730 - PEPD NNNCACCACCTACTTACCTG NGG 2 0.0702 Tier II
14 NC_000004.12 4 139470627 - RAB33B NNNCAGCACATAATTAGCTG NGG 2 0.0294 Tier II
15 NC_000013.11 13 39363791 + LHFPL6 NNNCACCACATAATTACCTG NCT 2 0.0 Tier II
16 NC_000001.11 1 167776343 - MPZL1 NNNCACCACATATTTTCCTG NGG 2 0.0 Tier II
17 NC_000009.12 9 41415147 - LOC107984035 NNNCACCACATAAATACCTA NGG 2 0.5804 Tier III
18 NC_000009.12 9 41415147 - LOC124900272 NNNCACCACATAAATACCTA NGG 2 0.5804 Tier III
19 NC_000021.9 21 10063581 + LOC105372733 NNNCACCACATAAATACCTA NGG 2 0.5804 Tier III
20 NC_000010.11 10 73507216 + PPP3CB-AS1 NNNCACTAGATAATTACCTG NGG 2 0.503 Tier III
21 NC_000001.11 1 200963271 - MROH3P NNNCACCACACAATTACCTT NGG 2 0.4667 Tier III
22 NC_000014.9 14 106209515 - IGH NNNCAGCAGATAATTACCTG NGG 2 0.3095 Tier III
23 NC_000003.12 3 180493566 - TTC14-DT NNNCACCATATTATTACCTG NGG 2 0.2917 Tier III
24 NC_000006.12 6 103536123 + LOC105377916 NNNCTCCACATGATTACCTG NGG 2 0.2626 Tier III
25 NC_000013.11 13 97149969 + LOC124903197 NNNCACCACAAAATCACCTG NGG 2 0.2045 Tier III
26 NC_000009.12 9 108198788 + LOC105376214 NNNCAGCACATAATTAGCTG NGG 2 0.0294 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 21060503 + Zfp182 NNNGACCACATACTTACCTG NGG 2 0.1053 Tier I
2 NC_000085.6 19 3921269 + Aldh3b1 NNNCACCACACAATTCCCTG NGG 2 0.0 Tier I
3 NC_000071.6 5 27041814 - Dpp6 NNNAACCACAGAATTACCTG NGG 2 0.3368 Tier II
4 NC_000071.6 5 98644352 - Cfap299 NNNCAGCACATATTTACCTG NGG 2 0.15 Tier II
5 NC_000077.6 11 57047645 + Gria1 NNNCAGCACATAATTGCCTG NGG 2 0.0962 Tier II
6 NC_000072.6 6 32564230 + Plxna4 NNNCACCACATAATTGACTG NGG 2 0.0897 Tier II
7 NC_000076.6 10 119576379 + Grip1 NNNCACCACATATTTACCTG NAG 2 0.0778 Tier II
8 NC_000079.6 13 42794070 + Phactr1 NNNCACCACATAACTTCCTG NGG 2 0.0 Tier II
9 NC_000086.7 X 162448645 + Reps2 NNNCGCCACATAATTCCCTG NGG 2 0.0 Tier II
10 NC_000071.6 5 13840871 - Gm8968 NNNTTCCACATAATTACCTG NGG 2 0.2909 Tier III
11 NC_000067.6 1 110600610 + Gm6693 NNNAACCACATATTTACCTG NGG 2 0.2526 Tier III
12 NC_000069.6 3 48608698 + 1700018B24Rik NNNCACCACACACTTACCTG NGG 2 0.1404 Tier III
13 NC_000068.7 2 9423109 + Gm13219 NNNCACCACATAATTTCCTT NGG 2 0.0 Tier III
14 NC_000067.6 1 158170012 - Gm39686 NNNCACCACATATTTTCCTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)