Construct: sgRNA BRDN0001487102
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCAGGCATTGATCATGATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPS2 (5634)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78006
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
12799300 |
- |
PRPS2 |
NNNAGGCATTGATCATGATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
6637334 |
+ |
DNAJC11 |
NNNAGGCATTGACGATGATG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000011.10 |
11 |
35198223 |
+ |
CD44 |
NNNAGGCATTGATGATGATG |
NAG |
2 |
0.0 |
Tier I |
| 4 |
NC_000007.14 |
7 |
143643751 |
- |
TCAF2 |
NNNAGACATTGACCATGATG |
NGG |
2 |
0.7895 |
Tier II |
| 5 |
NC_000007.14 |
7 |
143723587 |
+ |
TCAF2 |
NNNAGACATTGACCATGATG |
NGG |
2 |
0.7895 |
Tier II |
| 6 |
NC_000002.12 |
2 |
28768593 |
- |
PPP1CB |
NNNAGGCAGTGGTCATGATG |
NGG |
2 |
0.4471 |
Tier II |
| 7 |
NC_000006.12 |
6 |
159900638 |
- |
MAS1 |
NNNAGACTTTGATCATGATG |
NGG |
2 |
0.4286 |
Tier II |
| 8 |
NC_000002.12 |
2 |
65014420 |
+ |
SLC1A4 |
NNNAGGCATTGATAATGGTG |
NGG |
2 |
0.14 |
Tier II |
| 9 |
NC_000011.10 |
11 |
57742307 |
+ |
SELENOH |
NNNAGGCATTGATCTTGGTG |
NGG |
2 |
0.08 |
Tier II |
| 10 |
NC_000007.14 |
7 |
29204654 |
- |
CHN2 |
NNNAGGCATTGATCATGAGG |
NAG |
2 |
0.0648 |
Tier II |
| 11 |
NC_000003.12 |
3 |
72893910 |
- |
GXYLT2 |
NNNAGGCATGGATCATGATG |
NTG |
2 |
0.0195 |
Tier II |
| 12 |
NC_000007.14 |
7 |
143643751 |
- |
TCAF2C |
NNNAGACATTGACCATGATG |
NGG |
2 |
0.7895 |
Tier III |
| 13 |
NC_000007.14 |
7 |
143813893 |
+ |
LOC154761 |
NNNAGACATTGACCATGATG |
NGG |
2 |
0.7895 |
Tier III |
| 14 |
NC_000007.14 |
7 |
143813893 |
+ |
TCAF2P1 |
NNNAGACATTGACCATGATG |
NGG |
2 |
0.7895 |
Tier III |
| 15 |
NC_000011.10 |
11 |
57742307 |
+ |
TMX2-CTNND1 |
NNNAGGCATTGATCTTGGTG |
NGG |
2 |
0.08 |
Tier III |
| 16 |
NC_000007.14 |
7 |
29204654 |
- |
CHN2-AS1 |
NNNAGGCATTGATCATGAGG |
NAG |
2 |
0.0648 |
Tier III |
| 17 |
NC_000004.12 |
4 |
170387179 |
+ |
LINC02512 |
NNNAGGCATTGGTGATGATG |
NGG |
2 |
0.0 |
Tier III |
| 18 |
NC_000005.10 |
5 |
172757066 |
+ |
LOC101928093 |
NNNAGTCATTGATGATGATG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
5666309 |
+ |
Pcnx3 |
NNNAGGAATTGATCATGTTG |
NGG |
2 |
0.375 |
Tier I |
| 2 |
NC_000086.7 |
X |
167374094 |
+ |
Prps2 |
NNNACGCATTGATCATGATG |
NGT |
2 |
0.0127 |
Tier I |
| 3 |
NC_000068.7 |
2 |
102845309 |
- |
Cd44 |
NNNAGGCATTGATGATGATG |
NAG |
2 |
0.0 |
Tier I |
| 4 |
NC_000079.6 |
13 |
50470694 |
- |
Nutm2 |
NNNAGGCATAGGTCATGATG |
NGG |
2 |
0.619 |
Tier II |
| 5 |
NC_000076.6 |
10 |
64222432 |
- |
Ctnna3 |
NNNAGGTATTGAACATGATG |
NGG |
2 |
0.5625 |
Tier II |
| 6 |
NC_000068.7 |
2 |
135267705 |
- |
Plcb1 |
NNNAGGTATGGATCATGATG |
NGG |
2 |
0.4063 |
Tier II |
| 7 |
NC_000068.7 |
2 |
112781679 |
+ |
Ryr3 |
NNNAGTCATTGATCATGATG |
NAG |
2 |
0.1728 |
Tier II |
| 8 |
NC_000072.6 |
6 |
106413109 |
+ |
Cntn4 |
NNNCGGCATTGCTCATGATG |
NGG |
2 |
0.0929 |
Tier II |
| 9 |
NC_000069.6 |
3 |
127314163 |
+ |
Ank2 |
NNNAGGCATTGATCATGATG |
NGA |
1 |
0.0694 |
Tier II |
Other clones with same target sequence:
(none)
