Construct: shRNA TRCN0000003427

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174916.x-2048s1c1
DNA Barcode:
GCAGAGAAGAAATGTATGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBR1 (197131)
Gene Description:
ubiquitin protein ligase E3 component n-recognin 1
Transcript:
RefSeq NM_174916.1 (NON-CURRENT)
Match location:
Position 2048 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000003427)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAAGAAATGTATGATA
Hairpin Sequence:
5'-CCGG-GCAGAGAAGAAATGTATGATA-CTCGAG-TATCATACATTTCTTCTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAAGAAATGTATGATACTCGAGTATCATACATTTCTTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAAGAAATGTATGATACTCGAGTATCATACATTTCTTCTCTGC-3'

Other clones with same target sequence:

(none)