Construct: shRNA TRCN0000063055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033223.1-350s1c1
DNA Barcode:
CCTTCGATTCAACAGCACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRG3 (2567)
Gene Description:
gamma-aminobutyric acid type A receptor gamma3 subunit
Transcript:
RefSeq NM_033223.1 (NON-CURRENT)
Match location:
Position 350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000063055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCGATTCAACAGCACAAT
Hairpin Sequence:
5'-CCGG-CCTTCGATTCAACAGCACAAT-CTCGAG-ATTGTGCTGTTGAATCGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCGATTCAACAGCACAATCTCGAGATTGTGCTGTTGAATCGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCGATTCAACAGCACAATCTCGAGATTGTGCTGTTGAATCGAAGG-3'

Other clones with same target sequence:

(none)