Construct: shRNA TRCN0000094508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033586.2-1143s1c1
DNA Barcode:
GCCAAGGTTCTTGTAACGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcdhga3 (93711)
Gene Description:
protocadherin gamma subfamily A, 3
Transcript:
RefSeq NM_033586.2 (CURRENT)
Match location:
Position 1143 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000094508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGGTTCTTGTAACGGTT
Hairpin Sequence:
5'-CCGG-GCCAAGGTTCTTGTAACGGTT-CTCGAG-AACCGTTACAAGAACCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGGTTCTTGTAACGGTTCTCGAGAACCGTTACAAGAACCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGGTTCTTGTAACGGTTCTCGAGAACCGTTACAAGAACCTTGGC-3'

Other clones with same target sequence:

(none)