Construct: ORF TRCN0000465246
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006043.1_s317c1
- Derived from:
- ccsbBroadEn_01952
- DNA Barcode:
- CTAGCATGTCGTGCTTTATCCATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAPKAPK5 (8550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465246
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_003668.4 | 100% | 100% | |
| 2 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_139078.3 | 99.5% | 99.5% | 1216_1221delGGTAAA |
| 3 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371480.1 | 96.4% | 95.8% | (many diffs) |
| 4 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371479.1 | 96% | 95.4% | (many diffs) |
| 5 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371481.1 | 93.8% | 93.8% | 577_578ins81;1135_1140delGGTAAA |
| 6 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371483.1 | 93.6% | 93.6% | 392_393ins90 |
| 7 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371482.1 | 93.2% | 93.2% | 392_393ins90;1126_1131delGGTAAA |
| 8 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371484.1 | 92.3% | 86.3% | 184_185ins98;293_294insAGAT;1114_1119delGGTAAA |
| 9 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371486.1 | 85.3% | 85.3% | 184_185ins207 |
| 10 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371485.1 | 84.9% | 84.9% | 184_185ins207;1009_1014delGGTAAA |
| 11 | human | 8550 | MAPKAPK5 | MAPK activated protein kina... | NM_001371487.1 | 78.9% | 78.9% | 185_186ins297 |
| 12 | mouse | 17165 | Mapkapk5 | MAP kinase-activated protei... | NM_010765.2 | 90.3% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1479
- ORF length:
- 1413
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggaggagagc gacatggaca aagccatcaa ggaaacttcc attttagaag 121 aatacagtat caattggact cagaagctgg gagctggaat tagtggtcca gttagagtct 181 gtgtaaagaa atctactcaa gaacggtttg cgctgaaaat tcttcttgat cgtccaaaag 241 ctagaaatga ggtacgtctg cacatgatgt gtgccacaca cccaaacata gttcagatta 301 ttgaagtgtt tgctaacagt gtccagtttc cccatgagtc cagccctagg gcccgactct 361 taattgtaat ggagatgatg gaagggggag agctatttca cagaatcagc cagcaccggc 421 actttacaga gaagcaagcc agccaagtaa caaagcagat agctttggct ctgcggcact 481 gtcacttgtt aaacattgcg cacagagacc tcaagcctga aaatctgctt tttaaggata 541 actctttgga tgccccagtg aagttgtgtg actttggatt tgccaagatt gaccaaggtg 601 acttgatgac accccagttc accccttatt atgtagcacc ccaggtactg gaggcgcaaa 661 gaaggcatca gaaggagaaa tctggcatca tacctacctc accgacgccc tacacttaca 721 acaagagctg tgacttgtgg tccctagggg tgattatcta tgtgatgctg tgcggatacc 781 ctccttttta ctccaaacac cacagccgga ctatcccaaa ggatatgcga agaaagatca 841 tgacaggcag ttttgagttc ccagaggaag agtggagtca gatctcagag atggccaaag 901 atgttgtgag gaagctcctg aaggtcaaac cggaggagag actcaccatc gagggagtgc 961 tggaccaccc ctggctcaat tccaccgagg ccctggataa tgtgctgcct tctgctcagc 1021 tgatgatgga caaggcagtg gttgcaggaa tccagcaggc tcacgcggaa cagttggcca 1081 acatgagaat ccaggatctg aaagtcagcc tcaaacccct gcactcagtg aacaacccca 1141 ttctgcggaa gaggaagtta cttGGCACCA AGCCAAAGGA CAGTGTCTAT ATCCACGACC 1201 ATGAGAATGG AGCCGAGGAT TCCAATGTTG CCTTGGAAAA ACTCCGAGAT GTGATTGCTC 1261 AGTGTATTCT CCCCCAGGCT GGAGAGAATG AAGATGAGAA ACTGAATGAA GTAATGCAGG 1321 AGGCTTGGAA GTATAACCGG GAATGCAAAC TCCTAAGAGA TACTCTGCAG AGCTTCAGCT 1381 GGAATGGTCG TGGATTCACA GATAAAGTAG ATCGACTAAA ACTGGCAGAA ATTGTGAAGC 1441 AGGTGATAGA AGAGCAAACC ACGTCCCACG AATCCCAATA CCCAACTTTC TTGTACAAAG 1501 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1561 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1621 ACGACTAGCA TGTCGTGCTT TATCCATGAC GCGTTAAGTC gacaatcaac ctctggatta 1681 caaaatttgt gaaagatt