Construct: ORF TRCN0000465248
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011992.1_s317c1
- Derived from:
- ccsbBroadEn_12176
- DNA Barcode:
- CTGCCAGGTTTGACATAATTAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC61A2 (55176)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465248
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55176 | SEC61A2 | SEC61 translocon alpha 2 su... | NM_001142627.2 | 95.1% | 94.7% | (many diffs) |
| 2 | human | 55176 | SEC61A2 | SEC61 translocon alpha 2 su... | NM_018144.4 | 87.5% | 87.3% | (many diffs) |
| 3 | human | 55176 | SEC61A2 | SEC61 translocon alpha 2 su... | NM_001142628.1 | 82.9% | 82.7% | (many diffs) |
| 4 | human | 29927 | SEC61A1 | SEC61 translocon alpha 1 su... | NM_013336.4 | 68.8% | 81.5% | (many diffs) |
| 5 | human | 55176 | SEC61A2 | SEC61 translocon alpha 2 su... | NR_024576.2 | 55.5% | (many diffs) | |
| 6 | human | 55176 | SEC61A2 | SEC61 translocon alpha 2 su... | NR_024577.2 | 52.4% | (many diffs) | |
| 7 | mouse | 57743 | Sec61a2 | Sec61, alpha subunit 2 (S. ... | NM_021305.3 | 79.2% | 87.3% | (many diffs) |
| 8 | mouse | 57743 | Sec61a2 | Sec61, alpha subunit 2 (S. ... | XM_017319150.1 | 50.1% | 55% | (many diffs) |
| 9 | mouse | 57743 | Sec61a2 | Sec61, alpha subunit 2 (S. ... | XR_373943.2 | 46.8% | (many diffs) | |
| 10 | mouse | 57743 | Sec61a2 | Sec61, alpha subunit 2 (S. ... | XM_006497517.3 | 39.7% | 44.5% | (many diffs) |
| 11 | mouse | 57743 | Sec61a2 | Sec61, alpha subunit 2 (S. ... | XM_017319151.1 | 39.7% | 44.5% | (many diffs) |
| 12 | mouse | 57743 | Sec61a2 | Sec61, alpha subunit 2 (S. ... | XM_017319152.1 | 39.7% | 44.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg catcaaattt ttagaagtta tcaaaccatt ctgtgcagtt ctaccagaaa 121 ttcagaaacc ggaaaggaaa atccagttta gagagaaggt tctgtggact gctataacgc 181 tcttcatttt cttagtgtgt tgtcagatcc cactgtttgg aatcatgtca tcagattctg 241 cagatccttt ctactggatg agagttattc tggcttccaa tagaggaact ttaatggaat 301 tgggtatctc cccaattgta acatctggtt tgattatgca gttgttagct ggagccaaaa 361 tcattgaagt tggagataca ccgaaagata gagctttatt caatggagcc cagaaactgt 421 ttggtatgat cattaccatt gggcaagcca ttgtgtatgt catgacgggg atgtatgggg 481 accctgcaga aatgggtgct ggaatctgtc tcctgatcat cattcagttg tttgttgctg 541 gtttgattgt gctgctgtta gatgagctgc tacagaaggg ttacggcttg gggtctggga 601 tttccctctt tattgccacc aacatctgtg agaccattgt ctggaaggcc tttagtccca 661 ctaccattaa cactggcaga ggtactgagt ttgagggtgc agtcatagct ctgttccatt 721 tgttggccac caggacggac aaagtccgag ctttacggga ggctttttat cggcagaact 781 tacccaatct catgaacctc attgctacag tttttgtgtt tgctgttgtt atatatttcc 841 aaggatttcg cgttgatctg cccattaagt cggcccgtta ccgaggacag tacagcagct 901 accccatcaa actcttctac acctccaaca tccccatcaT CCTCCAGTCG GCCCTGGTGT 961 CCAACCTGTA TGTTATTTCC CAGATGCTGT CTGTTCGATT TAGTGGCAAC TTTTTAGTAA 1021 ATTTACTAGG ACAGTGGGCC GATGTCAGTG GGGGAGGACC CGCACGTTCT TACCCAGTTG 1081 GAGGCCTTTG TTACTATCTT TCTCCTCCTG AGTCCATGGG CGCCATCTTT GAGGATCCTG 1141 TCCATGTCGT TGTTTATATC ATCTTCATGT TGGGGTCATG TGCATTCTTC TCTAAGACAT 1201 GGATTGAAGT GTCTGGTTCC TCAGCCAAAG ATGTAGCTAA ACAGCTGAAA GAACAGCAGA 1261 TGGTAATGAG GGGCCACCGA GATACCTCTA TGGTTCATGA GCTTAATAGC GCTAGAGCGT 1321 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGACTGCC AGGTTTGACA TAATTAATTA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt