Construct: ORF TRCN0000465273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017596.1_s317c1
- Derived from:
- ccsbBroadEn_10391
- DNA Barcode:
- ACGGAGATAGTGCAGGTACTCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441394 | SUGT1P1 | SUGT1 pseudogene 1 | NR_003667.3 | 29.2% | 1_543del;784_820del | |
| 2 | human | 10910 | SUGT1 | SGT1 homolog, MIS12 kinetoc... | NM_001320831.2 | 24.7% | 17.4% | (many diffs) |
| 3 | human | 10910 | SUGT1 | SGT1 homolog, MIS12 kinetoc... | XM_017020366.2 | 22.1% | 16.8% | (many diffs) |
| 4 | human | 10910 | SUGT1 | SGT1 homolog, MIS12 kinetoc... | XM_017020367.1 | 22.1% | 16.8% | (many diffs) |
| 5 | human | 10910 | SUGT1 | SGT1 homolog, MIS12 kinetoc... | NM_006704.5 | 21.9% | 14.2% | (many diffs) |
| 6 | human | 10910 | SUGT1 | SGT1 homolog, MIS12 kinetoc... | XM_017020365.1 | 20% | 14% | (many diffs) |
| 7 | human | 10910 | SUGT1 | SGT1 homolog, MIS12 kinetoc... | NM_001130912.3 | 20% | 13.9% | (many diffs) |
| 8 | human | 10910 | SUGT1 | SGT1 homolog, MIS12 kinetoc... | XM_011534893.3 | 16.7% | 10.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 330
- ORF length:
- 240
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCCAGCCA GAAACGCATC TTTTTCTACA 121 TAGTTGCTGT TGCTGATGCA AAGAAGTCTC GCGAATTCAA TCCAAATAAT TCCACTGCTG 181 TGCTGAGAAA AGGGATATGT GAATACCATT TAAAAAACTA TGCTGCTGCT CTAGAAACTT 241 TTATAGGAGG ACAAAAATTA GTGCAGATGC TAATTTCAGT GACTGGATTA AAAGGTGTCA 301 AGAAGCTCAG AATGGCTCAG AATCTGAGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 361 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 421 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACGGA 481 GATAGTGCAG GTACTCAAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 541 tgaaagatt