Construct: ORF TRCN0000465283

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013588.1_s317c1
Derived from:
ccsbBroadEn_15366
DNA Barcode:
CGGCTGAAAAAAAGTGGGTGCGAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATP6V0C (527)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465283
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 527 ATP6V0C ATPase H+ transporting V0 s... NM_001198569.1 99.7% 100% 168C>T
2 human 527 ATP6V0C ATPase H+ transporting V0 s... NM_001694.4 99.7% 100% 168C>T
3 mouse 11984 Atp6v0c ATPase, H+ transporting, ly... NM_009729.3 86.6% 90.9% (many diffs)
4 mouse 100039636 Atp6v0c-ps2 ATPase, H+ transporting, ly... NR_037854.1 35.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
531
ORF length:
465
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc cgagtccaag agcggccccg agtatgcttc gtttttcgcc gtcatgggcg
121cctcggccgc catggtcttc agcgccctgg gcgctgccta tggcacagcc aagagcggta
181ccggcattgc ggccatgtct gtcatgcggc cggagcagat catgaagtcc attatcccag
241tggtcatggc tggcatcatc gccatcTACG GCCTGGTGGT GGCAGTCCTC ATCGCCAACT
301CCCTGAATGA CGACATCAGC CTCTACAAGA GCTTCCTCCA GCTGGGCGCC GGCCTGAGCG
361TGGGCCTGAG CGGCCTGGCA GCCGGCTTTG CCATCGGCAT CGTGGGGGAC GCTGGCGTGC
421GGGGCACCGC CCAGCAGCCC CGACTATTCG TGGGCATGAT CCTGATTCTC ATCTTCGCCG
481AGGTGCTCGG CCTCTACGGT CTCATCGTCG CCCTCATCCT CTCCACAAAG TACCCAACTT
541TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
601CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
661CTTGTGGAAA GGACGACGGC TGAAAAAAAG TGGGTGCGAG ACGCGTTAAG TCgacaatca
721acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)