Construct: ORF TRCN0000465290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017021.1_s317c1
- Derived from:
- ccsbBroadEn_03281
- DNA Barcode:
- CTCATCTTCGCTGTGGTCCTGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRCC1 (51315)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51315 | KRCC1 | lysine rich coiled-coil 1 | NM_001304526.2 | 100% | 100% | |
| 2 | human | 51315 | KRCC1 | lysine rich coiled-coil 1 | NM_016618.3 | 100% | 100% | |
| 3 | human | 51315 | KRCC1 | lysine rich coiled-coil 1 | XM_017004292.2 | 100% | 100% | |
| 4 | mouse | 57896 | Krcc1 | lysine-rich coiled-coil 1 | NM_145568.3 | 84.2% | 73.7% | (many diffs) |
| 5 | mouse | 57896 | Krcc1 | lysine-rich coiled-coil 1 | XM_006506429.3 | 84.2% | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcattcaaag aagacatatg actcttttca agatgaactt gaagattata 121 ttaaagtaca gaaagccaga ggcttagagc caaagacttg tttcagaaag atgaaagggg 181 actatttgga aacctgtggg tacaaaggag aggttaattc cagacccacg tatagaatgt 241 ttgaccagag actcccatct gaaaccatcc agacctaccc aagatcatgc aatattccac 301 aaacagtgga aaatcggttg cctcagtggt taccagccca tgacagcaga ttgagactag 361 actctctgag ctactgtcag ttcacgaggg actgtttctc agaaaaacca gtacccctga 421 actttaatca acaagaatat atttgtggct cacatggtgt agaacataga gtttacaagc 481 acttctccTC AGATAACAGT ACCAGTACTC ATCAAGCCAG TCACAAACAG ATACATCAGA 541 AGAGGAAAAG GCACCCAGAG GAAGGCAGAG AAAAATCAGA GGAGGAGCGG TCTAAGCATA 601 AGAGAAAAAA AAGCTGCGAG GAAATTGACT TAGACAAACA CAAGAGCATC CAAAGAAAGA 661 AAACAGAGGT GGAAATAGAA ACCGTACATG TCAGTACAGA AAAGCTTAAG AATCGAAAGG 721 AGAAAAAAAG CCGAGATGTA GTCTCTAAGA AAGAGGAACG TAAGCGTACA AAAAAGAAAA 781 AGGAACAAGG CCAAGAAAGG ACAGAGGAGG AAATGCTTTG GGACCAGTCT ATTCTTGGAT 841 TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 901 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 961 GGCTTTATAT ATCTTGTGGA AAGGACGACT CATCTTCGCT GTGGTCCTGT TGACGCGTTA 1021 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt