Construct: ORF TRCN0000465291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007643.1_s317c1
- Derived from:
- ccsbBroadEn_05928
- DNA Barcode:
- TGGTCACCCCCCTTCGGCACCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALM2 (805)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 805 | CALM2 | calmodulin 2 | NM_001743.6 | 99.7% | 99.3% | 370G>C |
| 2 | human | 801 | CALM1 | calmodulin 1 | NM_006888.6 | 85% | 99.3% | (many diffs) |
| 3 | human | 801 | CALM1 | calmodulin 1 | NM_001363670.1 | 84.4% | 98% | (many diffs) |
| 4 | human | 808 | CALM3 | calmodulin 3 | NM_001329922.1 | 81.4% | 99.3% | (many diffs) |
| 5 | human | 808 | CALM3 | calmodulin 3 | NM_005184.4 | 81.4% | 99.3% | (many diffs) |
| 6 | human | 805 | CALM2 | calmodulin 2 | NM_001305625.1 | 75.6% | 75.1% | 0_1ins108;262G>C |
| 7 | human | 805 | CALM2 | calmodulin 2 | NM_001305626.1 | 75.6% | 75.1% | 0_1ins108;262G>C |
| 8 | human | 805 | CALM2 | calmodulin 2 | NM_001305624.1 | 75.2% | 74.6% | 1_144del;146A>T;514G>C |
| 9 | human | 801 | CALM1 | calmodulin 1 | NM_001363669.1 | 64.6% | 75.1% | (many diffs) |
| 10 | human | 808 | CALM3 | calmodulin 3 | NM_001329921.1 | 61.5% | 75.1% | (many diffs) |
| 11 | human | 808 | CALM3 | calmodulin 3 | NM_001329923.1 | 61.5% | 75.1% | (many diffs) |
| 12 | human | 808 | CALM3 | calmodulin 3 | NM_001329924.2 | 61.5% | 75.1% | (many diffs) |
| 13 | human | 808 | CALM3 | calmodulin 3 | NM_001329925.2 | 61.5% | 75.1% | (many diffs) |
| 14 | human | 808 | CALM3 | calmodulin 3 | NM_001329926.2 | 61.5% | 75.1% | (many diffs) |
| 15 | mouse | 12314 | Calm2 | calmodulin 2 | NM_007589.5 | 91.7% | 99.3% | (many diffs) |
| 16 | mouse | 12313 | Calm1 | calmodulin 1 | NM_009790.5 | 83.6% | 99.3% | (many diffs) |
| 17 | mouse | 12315 | Calm3 | calmodulin 3 | NM_007590.3 | 81.2% | 99.3% | (many diffs) |
| 18 | mouse | 12314 | Calm2 | calmodulin 2 | NM_001355703.1 | 70.2% | 75.1% | (many diffs) |
| 19 | mouse | 12313 | Calm1 | calmodulin 1 | NM_001313934.1 | 62.4% | 74.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 513
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaccaactg actgaagagc agattgcaga attcaaagaa gctttttcac 121 tatttgacaa agatggtgat ggaactataa caacaaagga attgggaact gtaatgagat 181 ctcttgggca gaaTCCCACA GAAGCAGAGT TACAGGACAT GATTAATGAA GTAGATGCTG 241 ATGGTAATGG CACAATTGAC TTCCCTGAAT TTCTGACAAT GATGGCAAGA AAAATGAAAG 301 ACACAGACAG TGAAGAAGAA ATTAGAGAAG CATTCCGTGT GTTTGATAAG GATGGCAATG 361 GCTATATTAG TGCTGCAGAA CTTCGCCATG TGATGACAAA CCTTGGAGAG AAGTTAACAG 421 ATGAAGAAGT TGATCAAATG ATCAGGGAAG CAGATATTGA TGGTGATGGT CAAGTAAACT 481 ATGAAGAGTT TGTACAAATG ATGACAGCAA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 661 GTCACCCCCC TTCGGCACCT GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt