Transcript: Human NM_001305624.1

Homo sapiens calmodulin 2 (CALM2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
CALM2 (805)
Length:
1377
CDS:
83..676

Additional Resources:

NCBI RefSeq record:
NM_001305624.1
NBCI Gene record:
CALM2 (805)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001305624.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000052579 GTGATGGAACTATAACAACAA pLKO.1 297 CDS 100% 4.950 6.930 N CALM2 n/a
2 TRCN0000052580 GATGGTCAAGTAAACTATGAA pLKO.1 626 CDS 100% 5.625 4.500 N CALM2 n/a
3 TRCN0000333795 GATGGTCAAGTAAACTATGAA pLKO_005 626 CDS 100% 5.625 4.500 N CALM2 n/a
4 TRCN0000380676 AGATGGTGATGGAACTATAAC pLKO_005 292 CDS 100% 13.200 9.240 N CALM2 n/a
5 TRCN0000195431 CCAGGTACTCGTACACTATTT pLKO.1 1108 3UTR 100% 13.200 9.240 N CALM2 n/a
6 TRCN0000344827 CCAGGTACTCGTACACTATTT pLKO_005 1108 3UTR 100% 13.200 9.240 N CALM2 n/a
7 TRCN0000196827 GTACAAATGATGACAGCAAAG pLKO.1 653 CDS 100% 6.000 4.200 N CALM2 n/a
8 TRCN0000024691 CCCTGAATTTCTGACAATGAT pLKO.1 424 CDS 100% 5.625 3.938 N Calm2 n/a
9 TRCN0000052578 GCAGAGTTACAGGACATGATT pLKO.1 365 CDS 100% 5.625 3.938 N CALM2 n/a
10 TRCN0000052582 ACAAAGATGGTGATGGAACTA pLKO.1 288 CDS 100% 4.950 3.465 N CALM2 n/a
11 TRCN0000052581 AGAGAAGCATTCCGTGTGTTT pLKO.1 485 CDS 100% 4.950 3.465 N CALM2 n/a
12 TRCN0000024693 GTGTTTGATAAGGATGGCAAT pLKO.1 500 CDS 100% 4.050 2.835 N Calm2 n/a
13 TRCN0000024690 CGCCATGTGATGACAAACCTT pLKO.1 545 CDS 100% 3.000 2.100 N Calm2 n/a
14 TRCN0000338019 CGCCATGTGATGACAAACCTT pLKO_005 545 CDS 100% 3.000 2.100 N Calm2 n/a
15 TRCN0000338088 AGGATGGCAATGGCTATATTA pLKO_005 510 CDS 100% 15.000 9.000 N Calm2 n/a
16 TRCN0000196508 GAAGAGTTTGTACAAATGATG pLKO.1 644 CDS 100% 4.950 2.970 N CALM2 n/a
17 TRCN0000344826 GAAGAGTTTGTACAAATGATG pLKO_005 644 CDS 100% 4.950 2.970 N CALM2 n/a
18 TRCN0000196473 GATGAAGAAGTTGATGAAATG pLKO.1 581 CDS 100% 10.800 5.400 Y CALM2 n/a
19 TRCN0000344766 GATGAAGAAGTTGATGAAATG pLKO_005 581 CDS 100% 10.800 5.400 Y CALM2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001305624.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00207 pDONR223 100% 75.4% 75.1% None 1_144del;146A>T n/a
2 ccsbBroad304_00207 pLX_304 0% 75.4% 75.1% V5 1_144del;146A>T n/a
3 TRCN0000479230 GGATCATGATACTATTCGCCTACT pLX_317 100% 75.4% 75.1% V5 1_144del;146A>T n/a
4 ccsbBroadEn_14559 pDONR223 0% 75.4% 75.1% None 1_144del;146A>T n/a
5 ccsbBroad304_14559 pLX_304 0% 75.4% 75.1% V5 1_144del;146A>T n/a
6 TRCN0000481399 TTCTACTGACGAGTCGGCTAAACG pLX_317 100% 75.4% 75.1% V5 1_144del;146A>T n/a
7 ccsbBroadEn_05928 pDONR223 100% 75.2% 74.6% None 1_144del;146A>T;514G>C n/a
8 ccsbBroad304_05928 pLX_304 0% 75.2% 74.6% V5 1_144del;146A>T;514G>C n/a
9 TRCN0000465291 TGGTCACCCCCCTTCGGCACCTGG pLX_317 70.6% 75.2% 74.6% V5 1_144del;146A>T;514G>C n/a
10 ccsbBroadEn_05927 pDONR223 100% 64.2% 75.1% None (many diffs) n/a
11 ccsbBroad304_05927 pLX_304 0% 64.2% 75.1% V5 (many diffs) n/a
12 ccsbBroadEn_14558 pDONR223 0% 64.2% 75.1% None (many diffs) n/a
13 ccsbBroad304_14558 pLX_304 0% 64.2% 75.1% V5 (many diffs) n/a
14 TRCN0000491401 GTACCTCCCATCCAAAATTATCTA pLX_317 13.2% 64.1% 74.6% V5 (many diffs) n/a
15 ccsbBroadEn_00208 pDONR223 100% 61.5% 75.1% None (many diffs) n/a
16 ccsbBroad304_00208 pLX_304 0% 61.5% 75.1% V5 (many diffs) n/a
17 TRCN0000469481 ATCTGGCTTATGCACTTAGGCCTT pLX_317 72.8% 61.5% 75.1% V5 (many diffs) n/a
18 ccsbBroadEn_14560 pDONR223 0% 61.5% 75.1% None (many diffs) n/a
19 ccsbBroad304_14560 pLX_304 0% 61.5% 75.1% V5 (many diffs) n/a
20 TRCN0000479431 ATTAGCCGATGATTAAAGGATATG pLX_317 73.4% 61.5% 75.1% V5 (many diffs) n/a
21 ccsbBroadEn_15372 pDONR223 0% 57.3% 57.3% None 1_252del n/a
22 ccsbBroadEn_13821 pDONR223 100% 48.7% 54.8% None (many diffs) n/a
23 ccsbBroad304_13821 pLX_304 0% 48.7% 54.8% V5 (not translated due to frame shift) (many diffs) n/a
24 TRCN0000470188 ACTCTAACATCACAACCATTCACC pLX_317 100% 48.7% 54.8% V5 (not translated due to frame shift) (many diffs) n/a
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