Construct: ORF TRCN0000465292
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017281.1_s317c1
- Derived from:
- ccsbBroadEn_09695
- DNA Barcode:
- CTTGTGTCCGAAAAACGAACGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A48 (153328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465292
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153328 | SLC25A48 | solute carrier family 25 me... | NM_145282.4 | 99.5% | 100% | 66T>C;102G>A |
| 2 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_006714552.2 | 93.1% | 90% | (many diffs) |
| 3 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_017009091.1 | 64.9% | 61.9% | (many diffs) |
| 4 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_011543199.1 | 55.3% | 53.5% | (many diffs) |
| 5 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_005271903.1 | 54.7% | 52.9% | (many diffs) |
| 6 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_011543196.1 | 54.5% | 52.7% | (many diffs) |
| 7 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_006714550.1 | 54.3% | 52.5% | (many diffs) |
| 8 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_017009089.1 | 53.7% | 51.9% | (many diffs) |
| 9 | human | 153328 | SLC25A48 | solute carrier family 25 me... | NM_001349336.1 | 48.2% | 46.6% | (many diffs) |
| 10 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_006714546.1 | 48.2% | 46.6% | (many diffs) |
| 11 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_017009087.1 | 48.2% | 46.6% | (many diffs) |
| 12 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_017009088.1 | 48.2% | 46.6% | (many diffs) |
| 13 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XM_006714544.1 | 46.8% | 45.3% | (many diffs) |
| 14 | human | 153328 | SLC25A48 | solute carrier family 25 me... | NM_001349345.2 | 42.1% | 38.8% | (many diffs) |
| 15 | human | 153328 | SLC25A48 | solute carrier family 25 me... | NM_001349335.1 | 31.1% | 29.2% | (many diffs) |
| 16 | human | 153328 | SLC25A48 | solute carrier family 25 me... | NR_146141.2 | 27.2% | (many diffs) | |
| 17 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XR_001742004.1 | 6.1% | (many diffs) | |
| 18 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XR_001742005.1 | 6% | (many diffs) | |
| 19 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XR_001742007.1 | 6% | (many diffs) | |
| 20 | human | 153328 | SLC25A48 | solute carrier family 25 me... | XR_002959843.1 | 5.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aagcttccag ctggaagact ttgcggcggg ctggatcgga ggtgcagcca 121 gtgtcatcgt cggccaccct ctggacacag tcaagactcg cctgcaagct ggcgttggct 181 acggaaacac cctcagctgc atccgcgtgg tgtacaggag ggagagtatg ttcggcttct 241 tcaagggcat gtccttcccc ctcgccagca ttgccgtcta caactccgtg gtgtttgggg 301 tcttcagtaa cacgcagcgg ttccTCAGCC AGCACCGCTG CGGGGAGCCA GAGGCCAGTC 361 CTCCCCGCAC GCTGTCAGAC CTGCTCCTGG CCAGCATGGT GGCCGGCGTG GTCTCTGTCG 421 GGCTGGGAGG GCCCGTGGAC CTCATCAAGA TCCGGTTGCA GATGCAGACA CAACCGTTTC 481 GGGACGGAGG TGAACACAGG ATGACTACAG TGTTCCCTGG GCCTCATCTC TGCATGTGCC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GACTTGTGTC CGAAAAACGA ACGGCAACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt