Construct: ORF TRCN0000465315
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008383.1_s317c1
- Derived from:
- ccsbBroadEn_01866
- DNA Barcode:
- ATCCGACCTACTTATATGGAAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPRL3 (8131)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465315
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8131 | NPRL3 | NPR3 like, GATOR1 complex s... | NM_001039476.2 | 100% | 100% | |
| 2 | human | 8131 | NPRL3 | NPR3 like, GATOR1 complex s... | NM_001243247.1 | 79.4% | 79.4% | 1_303del |
| 3 | human | 8131 | NPRL3 | NPR3 like, GATOR1 complex s... | NM_001243248.1 | 71.6% | 71.6% | 1_462del |
| 4 | human | 8131 | NPRL3 | NPR3 like, GATOR1 complex s... | NM_001243249.1 | 71.6% | 71.6% | 1_462del |
| 5 | human | 8131 | NPRL3 | NPR3 like, GATOR1 complex s... | NM_001077350.3 | 68.5% | 68.5% | 1_537del |
| 6 | mouse | 17168 | Nprl3 | nitrogen permease regulator... | NM_001284360.1 | 76.7% | 83.7% | (many diffs) |
| 7 | mouse | 17168 | Nprl3 | nitrogen permease regulator... | NM_181569.3 | 60.5% | 66% | (many diffs) |
| 8 | mouse | 17168 | Nprl3 | nitrogen permease regulator... | NM_001284359.1 | 59.2% | 64.6% | (many diffs) |
| 9 | mouse | 17168 | Nprl3 | nitrogen permease regulator... | NR_104306.1 | 33.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgatggaaat gaaggtcctc agtccccatt ccatcacatc ctgcccaagt 121 gcaagctggc cagggacctc aaggaagctt atgacagcct gtgcacgtcg ggcgtagttc 181 ggcttcacat caacagctgg ctggaggtga gcttctgcct gccccacaag atccactatg 241 cggcctccag tctgatcccc ccagaggcca tcgaacggag cctgaaagcc atccgcccct 301 accatgccct gctgctgctc agtgatgaga agtccttgct gggtgagctt cctattgact 361 gctcccctgc cctagtgcgg gtgatcaaga ccacatctgc tgtgaagaac ctgcagcagc 421 tagcccaaga tgcggacctg gccttgctgc aggttttcca gcttgcagct catctggtgt 481 actggggcaa ggccatcatc atctacccgc tgtgtgagaa caacgtctac atgctgtctc 541 ccaatgccag cgtatgtctg tactccccgc tggccgagca gttctcccac cagttcccat 601 ctcatgacct gccgtccgtt cttgccaagt tctccttgcc ggtctccttg tcagaattta 661 ggaatcccct ggcccccgct gtgcaggaga cccagctcat ccagatggtg gtgtggatgc 721 tgcagcgccg gcttctcatc cagctgcaca cctatgtctg cctgatggcc tcacccagcg 781 aggaggagcc ccgtccgcga gaggacgacg tccccttcac tgcccgggtc ggcggtcgca 841 gcctcagcac gcccaacgcc cTCAGCTTTG GCTCCCCAAC CAGCAGCGAT GACATGACCC 901 TCACCAGCCC CAGCATGGAC AACTCCAGCG CAGAGCTACT TCCCAGCGGG GACTCGCCAC 961 TGAACCAGAG GATGACGGAG AACCTGCTGG CCAGCCTGTC GGAGCATGAA CGCGCAGCCA 1021 TCCTCAGTGT ACCCGCAGCC CAGAACCCTG AGGACCTCCG CATGTTTGCC AGGCTCCTTC 1081 ACTACTTCCG CGGCCGCCAC CACCTGGAGG AGATTATGTA CAACGAGAAC ACGCGGCGCT 1141 CCCAGCTGCT CATGCTGTTT GACAAGTTCC GCAGCGTGCT GGTGGTGACC ACCCACGAGG 1201 ACCCTGTCAT TGCCGTCTTC CAGGCTCTGC TCCCCTGCCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AATCCGACCT ACTTATATGG AAAGCACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt