Construct: ORF TRCN0000465316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016279.1_s317c1
- Derived from:
- ccsbBroadEn_12157
- DNA Barcode:
- CTTTACACTATGACCCAGCCACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPATCH2 (55105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55105 | GPATCH2 | G-patch domain containing 2 | NM_001297754.3 | 99.9% | 99.7% | 659G>C |
| 2 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XM_011509694.3 | 93.4% | 91.9% | (many diffs) |
| 3 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XM_017001593.2 | 89.3% | 86.1% | (many diffs) |
| 4 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XM_011509693.3 | 77.9% | 75.3% | (many diffs) |
| 5 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XM_017001592.2 | 76% | 73.4% | (many diffs) |
| 6 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XM_011509690.3 | 72.6% | 70.2% | (many diffs) |
| 7 | human | 55105 | GPATCH2 | G-patch domain containing 2 | NM_018040.5 | 70.7% | 68.4% | (many diffs) |
| 8 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XR_247030.4 | 69.8% | (many diffs) | |
| 9 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XM_011509689.3 | 67.7% | 65.5% | (many diffs) |
| 10 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XM_011509691.3 | 56.7% | 54.6% | (many diffs) |
| 11 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XR_921858.3 | 31.9% | (many diffs) | |
| 12 | human | 55105 | GPATCH2 | G-patch domain containing 2 | XR_921856.3 | 31.4% | (many diffs) | |
| 13 | mouse | 67769 | Gpatch2 | G patch domain containing 2 | XM_006497201.1 | 83.9% | 85.6% | (many diffs) |
| 14 | mouse | 67769 | Gpatch2 | G patch domain containing 2 | XM_006497195.2 | 55.4% | 54.9% | (many diffs) |
| 15 | mouse | 67769 | Gpatch2 | G patch domain containing 2 | XM_006497196.2 | 55.4% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cggggccgcc gggcgccaac cgatcggagc tccagcagcc gggaacagct 121 ggcatttcag tagaaccatg gaggagctgg ttcatgacct tgtctcagca ttggaagaga 181 gctcagagca agctcgaggt ggatttgctg aaacaggaga ccattctcga agtatatctt 241 gccctctgaa acgccaggca aggaaaagga gagggagaaa acggaggtcg tataatgtgc 301 atcacccgtg ggagactggt cactgcttaa gtgaaggctc tgattctagt ttagaagaac 361 caagcaagga ctatagagag aatcacaata ataataaaaa agatcacagt gactctgatg 421 accaaatgtt agtagcaaag cgcaggccgt catcaaactt aaataataat gttcgaggga 481 aaagacctct atggcatgag tctgattttg ctgtggacaa tgttgggaat agaactctgc 541 gcaggaggag aaaggtaaaa cgcatggcag tagatctccc acaggacatc tctaacaaac 601 ggacaatgac ccagccacct gagggttgta gagatcagga catggacagt gatagagcct 661 accagtatca agaatttacc aagaacaaag tcaaaaaaag aaagttgaaa ataatcagac 721 aagcaccaaa aatccaagat gaaggagtag ttttagaaag tgaggaaacg aaccagacca 781 ataaggacaa aatggaatgt gaagagcaaa aagtctcaga tgagctcatg agtgaaagtg 841 attccagcag tctcagcagc actgatgctg gattgtttac caatgatgag ggaagacaag 901 gtgatgatga acagagtgac tggttctacg aaaaggaatc aggtggagca tgtggtatca 961 ctGGAGTTGT GCCCTGGTGG GAAAAGGAAG ATCCTACTGA GCTAGACAAA AATGTACCAG 1021 ATCCTGTCTT TGAAAGTATC TTAACTGGTT CTTTTCCCCT TATGTCACAC CCAAGCAGAA 1081 GAGGTTTCCA AGCTAGACTC AGTCGCCTTC ATGGAATGTC TTCAAAGAAT ATTAAAAAAT 1141 CTGGAGGGAC TCCAACTTCA ATGGCTACAA ACTGGACCAG TGAGATTCCC CTATACCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAC TTTACACTAT GACCCAGCCA CCAACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att