Construct: ORF TRCN0000465321
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005874.1_s317c1
- Derived from:
- ccsbBroadEn_07250
- DNA Barcode:
- ACTGATACGCAAATTACATGTGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIPF (8513)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465321
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8513 | LIPF | lipase F, gastric type | NM_004190.4 | 99.8% | 99.7% | 481A>G;966A>G |
| 2 | human | 8513 | LIPF | lipase F, gastric type | XM_011540311.1 | 99.8% | 99.7% | 481A>G;966A>G |
| 3 | human | 8513 | LIPF | lipase F, gastric type | NM_001198829.2 | 97.3% | 97.3% | 1_30del;511A>G;996A>G |
| 4 | human | 8513 | LIPF | lipase F, gastric type | NM_001198828.2 | 91.5% | 91.4% | 220_221ins99;382A>G;867A>G |
| 5 | human | 8513 | LIPF | lipase F, gastric type | NM_001198830.2 | 89.2% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1263
- ORF length:
- 1194
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggctgctt ttaacaatgg caagtttgat atctgtactg gggactacac 121 atggtttgtt tggaaaatta catcctggaa gccctgaagt gactatgaac attagtcaga 181 tgattactta ttggggatac ccaaatgaag aatatgaagt tgtgactgaa gatggttata 241 ttcttgaagt caatagaatt ccttatggga agaaaaattc agggaataca ggccagagac 301 ctgttgtgtt tttgcagcat ggtttgcttg catcagccac aaactggatt tccaacctgc 361 cgaacaacag ccttgccttc attctggcag atgctggtta tgatgtgtgg ctgggcaaca 421 gcagaggaaa cacctgggcc agaagaaact tgtactattc accagattca gttgaattct 481 gggctttcag ctttgatgaa atggctaaat atgaccttcc agccacaatc gacttcattg 541 taaagaaagc tggacagaag cagctacact atgttggcca ttcccagggc accaccattg 601 gttttattgc cttttccacc aatcccagcc tggctaaaag aatcaaaacc ttctatgctc 661 tagctcctgt tgccactgtg aagtatacaa aaagccttat aaacaaactt agatttgttc 721 ctcaatccct cttcaagttt atatttggtg acaaaatatt ctacccacac aacttctttg 781 atcaatttct tgctactgaa gtgtgctccc gtgagatgct gaatctcctt tgcagcaatg 841 ccttatttat aatttgtgga tttgacagta agaactttaa cacgagtcgc ttggatgtgt 901 atctatcaca taatccagca ggaacttctg ttcaaaacat gttccattgg acccaggctg 961 ttaagtctgg gaaattccaa gcttatgact gggGAAGCCC AGTTCAGAAT AGGATGCACT 1021 ATGATCAGTC CCAGCCTCCC TACTACAATG TGACAGCCAT GAATGTACCA ATTGCAGTGT 1081 GGAACGGTGG CAAGGACCTG TTGGCTGACC CCCAAGATGT TGGCCTTTTG CTTCCAAAAC 1141 TCCCCAATCT TATTTACCAC AAGGAGATTC CTTTTTACAA TCACTTGGAC TTTATCTGGG 1201 CAATGGATGC CCCTCAAGAA GTTTACAATG ACATTGTTTC TATGATATCA GAAGATAAAA 1261 AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGAAC TGATACGCAA ATTACATGTG GAACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt