Transcript: Human NM_004190.4

Homo sapiens lipase F, gastric type (LIPF), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
LIPF (8513)
Length:
1391
CDS:
62..1258

Additional Resources:

NCBI RefSeq record:
NM_004190.4
NBCI Gene record:
LIPF (8513)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004190.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000051138 CCAGCCACAATCGACTTCATT pLKO.1 512 CDS 100% 5.625 4.500 N LIPF n/a
2 TRCN0000051141 CCAGAAGAAACTTGTACTATT pLKO.1 432 CDS 100% 13.200 9.240 N LIPF n/a
3 TRCN0000436939 CCATTGGACCCAGGCTGTTAA pLKO_005 937 CDS 100% 13.200 9.240 N LIPF n/a
4 TRCN0000051139 CCTGAAGTGACTATGAACATT pLKO.1 146 CDS 100% 5.625 3.938 N LIPF n/a
5 TRCN0000051142 CCACACAACTTCTTTGATCAA pLKO.1 758 CDS 100% 4.950 3.465 N LIPF n/a
6 TRCN0000178813 CAGCTTTGATGAAATGGCTAA pLKO.1 481 CDS 100% 4.050 2.835 N Lipf n/a
7 TRCN0000051140 TGGATGTGTATCTATCACATA pLKO.1 885 CDS 100% 0.495 0.347 N LIPF n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004190.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07250 pDONR223 100% 99.8% 99.7% None 481A>G;966A>G n/a
2 ccsbBroad304_07250 pLX_304 0% 99.8% 99.7% V5 481A>G;966A>G n/a
3 TRCN0000465321 ACTGATACGCAAATTACATGTGGA pLX_317 23% 99.8% 99.7% V5 481A>G;966A>G n/a
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