Construct: ORF TRCN0000465330

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012727.1_s317c1
Derived from:
ccsbBroadEn_09260
DNA Barcode:
CGATGGGTATCCCTGACTGTTAGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EAF1 (85403)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465330
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 85403 EAF1 ELL associated factor 1 NM_033083.7 99.6% 99.2% 186G>T;290A>T;754A>G
2 human 85403 EAF1 ELL associated factor 1 XM_005265518.2 62% 58.2% (many diffs)
3 human 85403 EAF1 ELL associated factor 1 XM_011534165.1 62% 58.2% (many diffs)
4 human 85403 EAF1 ELL associated factor 1 XM_011534166.1 62% 58.2% (many diffs)
5 mouse 74427 Eaf1 ELL associated factor 1 NM_028932.4 88.6% 97.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
870
ORF length:
804
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa tgggaccgca aacccgctgc tggaccgcga ggaacattgc ctgaggctcg
121gggagagctt cgagaagcgg ccgcgggcct ccttccacac tattcgttat gattttaaac
181cagcatctat agacacttcc tgtgaaggag agcttcaagt tggcaaagga gatgaagtca
241caattacact tccacatatc cctggatcca caccacccat gactgtgttc aaggggaaca
301aacggcctta ccagaaagac tgtgtgctta ttattaatca tgacactggt gaatttgtgc
361tggaaaaact cagtagcagc attcaggtga agaaaacaag agctgagggc agcagtaaaa
421tccaggcccg aatggaacag cagcccactc gtcctccaca gacgtcacag ccaccaccac
481ctccaccacc TATGCCATTC AGAGCTCCAA CGAAGCCTCC AGTTGGACCC AAAACTTCTC
541CCTTGAAAGA TAACCCCTCA CCTGAACCTC AGTTGGATGA CATCAAAAGA GAGCTGAGGG
601CTGAAGTTGA CATTATTGAA CAAATGAGCA GCAGCAGTGG GAGCAGCTCT TCAGACTCTG
661AGAGCTCTTC GGGAAGTGAT GACGATAGCT CCAGCAGTGG AGGCGAGGAC AATGGCCCAG
721CCTCTCCTCC GCAGCCTTCA CACCAGCAGC CCTACAACAG TAGGCCTGCC GTTGCCAATG
781GAACCAGCCG GCCACAAGGA AGCAACCAGC TCATGAACGC CCTCAGAAAT GACTTGCAGT
841TGAGTGAGTC TGGCAGTGAC AGTGATGACT ACCCAACTTT CTTGTACAAA GTGGTTGATA
901TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
961CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGATG
1021GGTATCCCTG ACTGTTAGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1081tgaaagatt

Download FASTA (ORF) (Full)