Construct: ORF TRCN0000465332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006513.2_s317c1
- Derived from:
- ccsbBroadEn_09164
- DNA Barcode:
- ATTGTATTCATGGAATAGACGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMKK1 (84254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | NM_172207.2 | 99.8% | 99.8% | 15A>G;1170A>G;1238A>G |
| 2 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_005256817.1 | 95.5% | 95.5% | (many diffs) |
| 3 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_006721588.3 | 95.5% | 95.5% | (many diffs) |
| 4 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524031.3 | 94.8% | 94.8% | (many diffs) |
| 5 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450996.1 | 91% | 91% | (many diffs) |
| 6 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | NM_032294.3 | 88.5% | 88.5% | (many diffs) |
| 7 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_017025220.2 | 88.5% | 88.5% | (many diffs) |
| 8 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | NM_172206.2 | 84.3% | 84.3% | (many diffs) |
| 9 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XR_001752661.2 | 72.9% | (many diffs) | |
| 10 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450997.1 | 50.4% | 47% | (many diffs) |
| 11 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524032.3 | 50% | 50% | 1_81del;96A>G;903_904ins738 |
| 12 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524033.1 | 44.8% | 44.7% | (many diffs) |
| 13 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_011524035.3 | 44.8% | 44.7% | (many diffs) |
| 14 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450998.1 | 44.8% | 44.7% | (many diffs) |
| 15 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XM_024450999.1 | 44.8% | 44.7% | (many diffs) |
| 16 | human | 84254 | CAMKK1 | calcium/calmodulin dependen... | XR_001752662.2 | 37.4% | (many diffs) | |
| 17 | mouse | 55984 | Camkk1 | calcium/calmodulin-dependen... | NM_018883.2 | 78.8% | 82.8% | (many diffs) |
| 18 | mouse | 55984 | Camkk1 | calcium/calmodulin-dependen... | XM_006533760.3 | 78.8% | 82.8% | (many diffs) |
| 19 | mouse | 55984 | Camkk1 | calcium/calmodulin-dependen... | XM_006533761.3 | 78.8% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1626
- ORF length:
- 1560
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggggggtccg gctgtctgct gccaggatcc tcgggcagag ctggtagaac 121 gggtggcagc catcgatgtg actcacttgg aggaggcaga tggtggccca gagcctacta 181 gaaacggtgt ggacccccca ccacgggcca gagctgcctc tgtgatccct ggcagtactt 241 caagactgct cccagcccgg cctagcctct cagccaggaa gctttcccta caggagcggc 301 cagcaggaag ctatctggag gcgcaggctg ggccttatgc cacggggcct gccagccaca 361 tctccccccg ggcctggcgg aggcccacca tcgagtccca ccacgtggcc atctcagatg 421 cagaggactg cgtgcagctg aaccagtaca agctgcagag tgagattggc aagggtgcct 481 acggtgtggt gaggctggcc tacaacgaaa gtgaagacag acactatgca atgaaagtcc 541 tttccaaaaa gaagttactg aagcagtatg gctttccacg tcgccctccc ccgagagggt 601 cccaggctgc ccagggagga ccagccaagc agctgctgcc cctggagcgg gtgtaccagg 661 agattgccat cctgaagaag ctggaccacg tgaatgtggt caaactgatc gaggtcctgg 721 atgacccagc tgaggacaac ctctatttgg ccctgcagaa ccaggcccag aatatccagt 781 tagattcaac aaatatcgcc aagccccact ccctgcttcc ctctgagcag caagacagtg 841 gatccacgtg ggctgcgcgc tcagtgtttg acctcctgag aaaggggccc gtcatggaag 901 tgccctgtga caagcccttc tcggaggagc aagctcgcct ctacctgcgg gacgtcatcc 961 tgggcctcga gtacttgcac tgccagaaga tcgtccacag ggacatcaag ccatccaacc 1021 tgctcctggg ggatgatggg cacgtgaaga tcgccgactt tggcgtcagc aaccagtttg 1081 aggggaacga cgctcagctg tccagcacgg cgggaacccc agcattcatg gcccccgagg 1141 ccatttctga ttccggccag agcttcagtg ggaaggcctt ggatgtatgg gccactggcg 1201 tcacgttgta ctgctttgtc tatgggaagt gcccgttcat cgacgatttc atcctggCCC 1261 TCCACAGGAA GATCAAGAAT GAGCCCGTGG TGTTTCCTGA GGGGCCAGAA ATCAGCGAGG 1321 AGCTCAAGGA CCTGATCCTG AAGATGTTAG ACAAGAATCC CGAGACGAGA ATTGGGGTGC 1381 CAGACATCAA GTTGCACCCT TGGGTGACCA AGAACGGGGA GGAGCCCCTT CCTTCGGAGG 1441 AGGAGCACTG CAGCGTGGTG GAGGTGACAG AGGAGGAGGT TAAGAACTCA GTCAGGCTCA 1501 TCCCCAGCTG GACCACGGTG ATCCTGGTGA AGTCCATGCT GAGGAAGCGT TCCTTTGGGA 1561 ACCCGTTTGA GCCCCAAGCA CGGAGGGAAG AGCGATCCAT GTCTGCTCCA GGAAACCTAC 1621 TGGTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1681 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1741 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATTGTATTC ATGGAATAGA CGTCAACGCG 1801 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt