Construct: ORF TRCN0000465340
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002098.1_s317c1
- Derived from:
- ccsbBroadEn_01134
- DNA Barcode:
- AACGATCCGCACGGGGCTGGTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P2RX5 (5026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465340
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5026 | P2RX5 | purinergic receptor P2X 5 | NM_002561.4 | 100% | 100% | |
| 2 | human | 5026 | P2RX5 | purinergic receptor P2X 5 | NM_001204519.2 | 99.7% | 99.5% | 614_615insAAG |
| 3 | human | 5026 | P2RX5 | purinergic receptor P2X 5 | NM_001204520.2 | 94.3% | 94.3% | 286_287ins72 |
| 4 | human | 5026 | P2RX5 | purinergic receptor P2X 5 | NM_175080.3 | 94% | 93.8% | 286_287ins72;542_543insAAG |
| 5 | human | 100533970 | P2RX5-TAX1BP3 | P2RX5-TAX1BP3 readthrough (... | NR_037928.1 | 20.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1332
- ORF length:
- 1266
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gcaggcgggc tgcaaggggc tctgcctgtc gctgttcgac tacaagaccg 121 agaagtatgt catcgccaag aacaagaagg tgggcctgct gtaccggctg ctgcaggcct 181 ccatcctggc gtacctggtc gtatgggtgt tcctgataaa gaagggttac caagacgtcg 241 acacctccct gcagagtgct gtcatcacca aagtcaaggg cgtggccttc accaacacct 301 cggatcttgg gcagcggatc tgggatgtcg ccgactacgt cattccagcc cagggagaga 361 acgtcttttt tgtggtcacc aacctgattg tgacccccaa ccagcggcag aacgtctgtg 421 ctgagaatga aggcattcct gatggcgcgt gctccaagga cagcgactgc cacgctgggg 481 aagcggttac agctggaaac ggagtgaaga ccggccgctg cctgcggaga gagaacttgg 541 ccaggggcac ctgtgagatc tttgcctggt gcccgttgga gacaagctcc aggccggagg 601 agccattcct gaaggaggcc gaagacttca ccattttcat aaagaaccac atccgtttcc 661 ccaaattcaa cttctccaaa agcaatgtga tggacgtcaa ggacagatct ttcctgaaat 721 catgccactt tggccccaag aaccactact gccccatctt ccgactgggc tccgtgatcc 781 gctgggccgg gagcgacttc caggatatag ccctggaggg tggcgtgata ggaattaata 841 ttgaatggaa ctgtgatctt gataaagctg cctctgagtg ccaccctcac tattctttta 901 gccgtctgga caataaactt tcaaagtctg tctcctccgg gtacaacttc agatttgcca 961 gatattaccg agacgcagcc ggggtggagt tccgcaccct gatgaaagcc TACGGGATCC 1021 GCTTTGACGT GATGGTGAAC GGCAAGGGTG CTTTCTTCTG CGACCTGGTA CTCATCTACC 1081 TCATCAAAAA GAGAGAGTTT TACCGTGACA AGAAGTACGA GGAAGTGAGG GGCCTAGAAG 1141 ACAGTTCCCA GGAGGCCGAG GACGAGGCAT CGGGGCTGGG GCTATCTGAG CAGCTCACAT 1201 CTGGGCCAGG GCTGCTGGGG ATGCCGGAGC AGCAGGAGCT GCAGGAGCCA CCCGAGGCGA 1261 AGCGTGGAAG CAGCAGTCAG AAGGGGAACG GATCTGTGTG CCCACAGCTC CTGGAGCCCC 1321 ACAGGAGCAC GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1381 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1441 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC GATCCGCACG GGGCTGGTTC 1501 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t