Construct: ORF TRCN0000465352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017485.1_s317c1
- Derived from:
- ccsbBroadEn_12786
- DNA Barcode:
- GATGGCAATATAAGTGATGTTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GTF2IRD2 (84163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001003795.3 | 49.4% | 44.1% | (many diffs) |
| 2 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_173537.4 | 49.3% | 44.1% | (many diffs) |
| 3 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001368302.1 | 42.2% | 37.8% | (many diffs) |
| 4 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_001368300.1 | 42.2% | 37.8% | (many diffs) |
| 5 | human | 84163 | GTF2IRD2 | GTF2I repeat domain contain... | NM_001281447.1 | 19.8% | 16.8% | (many diffs) |
| 6 | human | 389524 | GTF2IRD2B | GTF2I repeat domain contain... | NM_001368301.1 | 19.8% | 16.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1560
- ORF length:
- 1494
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccaggtagca gtgtccaccc tgcctgttga agaagagtcc tcctcagaga 121 ccaggatggt ggtgacattc ctcgtgtctg ccctcgaatc catgtgtaaa gaactggcca 181 agtccaaggc agaagtggcc tgcatcgcag tgtacgaaac agacgtgttt gtcgtcggaa 241 ccgagagagg atgcgctttt gttaatgcca ggacggattt tcagaaagat tttgcaaaat 301 actgcgttgc agagggactg tgtgaggtga aacctccctg ccctgtgaac gggatgcagg 361 tccactcggg cgaaacggaa atactcagga aggcagtgga ggactatttc tgcttttgtt 421 atggtaaagc cttagggaca acagtgatgg tgcctgttcc ctatgagaag atgctgcgag 481 accagtcggc tgtggtagtg caggggcttc cggaaggcgt tgcctttcaa caccctgaga 541 attacgacct tgcaaccctg aaatggattt tggagaacaa agcagggatt tcattcatca 601 taaatagacc cttcctagga ccagagagtc agctgggtgg ccctgggatg gtaacagatg 661 cggagagatc catagtatca ccaagtgaaa gctgcggccc catcaatgtg aaaactgaac 721 ccatggaaga ttctggcatt tcactgaaag cagaagctgt ctcagtcaag aaagaatcag 781 aagatcctaa ttactatcaa tataatatgc aaggaagcca cccttcttcc acaagcaatg 841 aagtaataga aatggaatta ccaatggaag attccactcc gctggtccct tcagaagaac 901 caaatgagga ccctgaagcc gaggtgaaaa tcgaaggaaa cacaaattca tccagtgtta 961 caaattctgc agcaggtgtt gaagatctta acatcgttca agtgactgtt ccagataatg 1021 agaaggaaag attatcaagc attgaaaaga ttaaacagct aagagaacaa gttaatgacc 1081 tctttagccg aaaatttggt gaagcaattg gcgtggattt ccctgtgaaa gttccctaca 1141 ggaagatcac attcaaccct ggctgtgtgg tgattgatgg catgcccccg ggggtggtat 1201 tcaaggcccc cggctatctg gaaatcagtt ccatgaggag gatcttGGAG GCAGCTGAGT 1261 TTATCAAATT CACAGTCATC AGGCCGCTTC CAGGGCTTGA GCTCAGTAAT GGACTTTTGG 1321 AGGCCGGGGC AGGAGGATTG CTTGAGGCCG GGAGTTTGAG GCCAGCCTGG GCAACATGGC 1381 AAGACCCCAT CTCTACAAAA AATTTAAAAA TTAGCTGGAA ACAGTGGCTC ACACCTGTAG 1441 CCCCAGCTAC TTGGCAGGCT GAAGCGGGAG GATCGCTTGA ACTCAGGAGT TTGAGACAGC 1501 AGAGAACCAT AATTGCAATA CTGCACTCCA GCCTGGGTGA CAGAACAAGA CCCTGTCTCT 1561 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1621 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1681 TTTATATATC TTGTGGAAAG GACGAGATGG CAATATAAGT GATGTTCTCA CGCGTTAAGT 1741 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt