Transcript: Human NM_001368302.1

Homo sapiens GTF2I repeat domain containing 2B (GTF2IRD2B), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-04-16
Taxon:
Homo sapiens (human)
Gene:
GTF2IRD2B (389524)
Length:
4373
CDS:
518..3853

Additional Resources:

NCBI RefSeq record:
NM_001368302.1
NBCI Gene record:
GTF2IRD2B (389524)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001368302.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000422525 CACTCGGGCGAAACGGAAATA pLKO_005 1301 CDS 100% 13.200 7.920 N GTF2IRD2 n/a
2 TRCN0000020991 CGTGTTGAGAAGAGCCTGAAA pLKO.1 2795 CDS 100% 4.950 2.970 N GTF2IRD2 n/a
3 TRCN0000155815 CCCTGAAATTGGTTTCCAGAA pLKO.1 3426 CDS 100% 4.050 2.430 N GTF2IRD2B n/a
4 TRCN0000435626 TCGATGAGATCACGGATATAA pLKO_005 2655 CDS 100% 15.000 7.500 Y GTF2IRD2 n/a
5 TRCN0000020993 CCTGTACTACACGGAGATTAA pLKO.1 3118 CDS 100% 13.200 6.600 Y GTF2IRD2 n/a
6 TRCN0000020989 GCAAGCATTATGACCAGTATA pLKO.1 2415 CDS 100% 13.200 6.600 Y GTF2IRD2 n/a
7 TRCN0000157406 GCCCTCGAATCCATGTGTAAA pLKO.1 1088 CDS 100% 13.200 6.600 Y GTF2IRD2B n/a
8 TRCN0000151243 GCAGGGATTTCATTCATCATA pLKO.1 1520 CDS 100% 5.625 2.813 Y GTF2IRD2B n/a
9 TRCN0000151754 CGCAAAGATTCTTTCCATGTT pLKO.1 3718 CDS 100% 4.950 2.475 Y GTF2IRD2B n/a
10 TRCN0000155187 GAGATGAGGTTTCACCATGTT pLKO.1 4118 3UTR 100% 4.950 2.475 Y GTF2IRD2B n/a
11 TRCN0000157407 GATCTCGGCTTACTGCAACTT pLKO.1 4000 3UTR 100% 4.950 2.475 Y GTF2IRD2B n/a
12 TRCN0000154764 GCGTATTTCTTCGTGGAAGTA pLKO.1 2306 CDS 100% 4.950 2.475 Y GTF2IRD2B n/a
13 TRCN0000020990 GCTGTCTGATTTCAAACTCTA pLKO.1 3511 CDS 100% 4.950 2.475 Y GTF2IRD2 n/a
14 TRCN0000155454 GCGCAAAGATTCTTTCCATGT pLKO.1 3717 CDS 100% 4.050 2.025 Y GTF2IRD2B n/a
15 TRCN0000157719 CCTGAGAATTACGACCTTGCA pLKO.1 1472 CDS 100% 2.640 1.320 Y GTF2IRD2B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001368302.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14494 pDONR223 100% 85.3% 85% None 1_486del;3048C>T;3323_3324insC n/a
2 ccsbBroad304_14494 pLX_304 0% 85.3% 85% V5 (not translated due to frame shift) 1_486del;3048C>T;3323_3324insC n/a
3 TRCN0000475879 CAAGAACGGTTTTTTCTGCCATGC pLX_317 12.2% 85.3% 85% V5 (not translated due to prior stop codon) 1_486del;3048C>T;3323_3324insC n/a
4 ccsbBroadEn_12786 pDONR223 100% 42.2% 37.8% None (many diffs) n/a
5 ccsbBroad304_12786 pLX_304 0% 42.2% 37.8% V5 (many diffs) n/a
6 TRCN0000465352 GATGGCAATATAAGTGATGTTCTC pLX_317 21.4% 42.2% 37.8% V5 (many diffs) n/a
7 ccsbBroadEn_12785 pDONR223 100% 21.8% 20.6% None (many diffs) n/a
8 ccsbBroad304_12785 pLX_304 0% 21.8% 20.6% V5 (many diffs) n/a
9 TRCN0000469667 CGATCACCGATGTAAGTTTATTTC pLX_317 48.6% 21.8% 20.6% V5 (many diffs) n/a
Download CSV

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