Construct: ORF TRCN0000465365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017297.1_s317c1
- Derived from:
- ccsbBroadEn_07241
- DNA Barcode:
- GTCTTCGCCATCGCGTTCTGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DOC2A (8448)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8448 | DOC2A | double C2 domain alpha | NM_001282062.1 | 99.8% | 99.7% | 142G>A;648G>A |
| 2 | human | 8448 | DOC2A | double C2 domain alpha | NM_001282063.1 | 99.8% | 99.7% | 142G>A;648G>A |
| 3 | human | 8448 | DOC2A | double C2 domain alpha | NM_001282068.2 | 99.8% | 99.7% | 142G>A;648G>A |
| 4 | human | 8448 | DOC2A | double C2 domain alpha | NM_003586.3 | 99.8% | 99.7% | 142G>A;648G>A |
| 5 | human | 8448 | DOC2A | double C2 domain alpha | XM_011545975.1 | 99.8% | 99.7% | 142G>A;648G>A |
| 6 | human | 8448 | DOC2A | double C2 domain alpha | XM_024450473.1 | 99.8% | 99.7% | 142G>A;648G>A |
| 7 | human | 8448 | DOC2A | double C2 domain alpha | XM_024450474.1 | 99.8% | 99.7% | 142G>A;648G>A |
| 8 | human | 8448 | DOC2A | double C2 domain alpha | XM_024450472.1 | 85.5% | 85.4% | 142G>A;341_541del;849G>A |
| 9 | human | 8448 | DOC2A | double C2 domain alpha | XM_017023778.1 | 70.4% | 70.5% | 0_1ins354;294G>A |
| 10 | human | 8448 | DOC2A | double C2 domain alpha | NR_104089.2 | 56.6% | (many diffs) | |
| 11 | human | 8448 | DOC2A | double C2 domain alpha | NR_104090.2 | 53.3% | (many diffs) | |
| 12 | mouse | 13446 | Doc2a | double C2, alpha | NM_010069.1 | 86.5% | 93.8% | (many diffs) |
| 13 | mouse | 13446 | Doc2a | double C2, alpha | XM_017321966.1 | 86.5% | 93.8% | (many diffs) |
| 14 | mouse | 13446 | Doc2a | double C2, alpha | XM_017321967.1 | 86.5% | 93.8% | (many diffs) |
| 15 | mouse | 13446 | Doc2a | double C2, alpha | XM_017321968.1 | 86.5% | 93.8% | (many diffs) |
| 16 | mouse | 13446 | Doc2a | double C2, alpha | XM_017321969.1 | 86.5% | 93.8% | (many diffs) |
| 17 | mouse | 13446 | Doc2a | double C2, alpha | XM_017321970.1 | 86.5% | 93.8% | (many diffs) |
| 18 | mouse | 13446 | Doc2a | double C2, alpha | XM_017321971.1 | 86.5% | 93.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gggccgcagg ggcgatcgca tgaccatcaa catccaggag cacatggcca 121 tcaacgtgtg ccccgggccc atccggccca tccgccagat ctctgactac ttcccccggg 181 gaccaggacc tgaagggggc ggcgggagcg gcggggaggc ccccgcccat ctggtccccc 241 tggctctggc cccccctgca gccctccttg gggccaccac gcctgaggat ggtgcggagg 301 tggacagcta tgactcggat gatgccaccg ccctaggcac gctggagttt gaccttctct 361 acgaccgggc ctcctgcact ctgcactgta gcatcctcag ggccaagggc ctcaagccca 421 tggatttcaa tggcctcgcc gacccctacg tcaagctgca cttgctgcct ggagcctgta 481 aggccaataa gctaaaaacg aagactcaga ggaacacact gaatcccgtg tggaatgagg 541 acctgactta cagcgggatc acagatgacg acatcacgca caaggtgctc aggatcgccg 601 tctgtgatga ggacaagctg agtcacaatg agtttattgg ggagatccgc gtgcccctcc 661 gccgcctcaa gccttcgcag aagaagcatt ttaacatctg cctcgagcgc caagtcccgc 721 tggcgtcccc ctcttccatg tcagcggcgc tgaggggcat ctcctgttat ctgaaggagt 781 tggagcaggc ggagcagggg caggggctgc tggaggagcg tggccgcatc ctgctgagtc 841 tcagctacag ctcgcggcgc cggggactgc tggtaggcat cttgcgctgc gcccatctgg 901 ctgccatgga cgtcaacggt tactcggacc cctacgtcaa gacgtaccTG AGGCCCGATG 961 TGGACAAGAA ATCCAAGCAT AAGACGTGTG TGAAGAAGAA GACTCTCAAC CCAGAATTTA 1021 ACGAGGAGTT TTTCTACGAG ATAGAGCTCT CCACTCTGGC CACCAAGACC CTGGAAGTCA 1081 CCGTCTGGGA CTATGACATT GGCAAATCCA ATGACTTCAT TGGTGGCGTG TCCCTGGGGC 1141 CAGGTGCCCG AGGCGAGGCT CGGAAGCACT GGAGTGACTG CCTGCAGCAG CCGGACGCAG 1201 CCCTGGAGCG CTGGCACACC CTGACCAGTG AGCTGCCCCC TGCGGCCGGG GCTCTGTCCT 1261 CAGCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTCTTCGCC ATCGCGTTCT GGAACACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt