Construct: ORF TRCN0000465374
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011258.1_s317c1
- Derived from:
- ccsbBroadEn_06989
- DNA Barcode:
- AAGCCATATTTCATAACCAGGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPINT1 (6692)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465374
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6692 | SPINT1 | serine peptidase inhibitor,... | NM_001032367.2 | 99.8% | 100% | 1227C>T;1530G>C |
| 2 | human | 6692 | SPINT1 | serine peptidase inhibitor,... | NM_003710.4 | 99.8% | 100% | 1227C>T;1530G>C |
| 3 | human | 6692 | SPINT1 | serine peptidase inhibitor,... | NM_181642.3 | 96.8% | 96.9% | 913_960del;1275C>T;1578G>C |
| 4 | human | 6692 | SPINT1 | serine peptidase inhibitor,... | XM_006720657.1 | 96.8% | 96.9% | 913_960del;1275C>T;1578G>C |
| 5 | human | 6692 | SPINT1 | serine peptidase inhibitor,... | XM_011521957.2 | 93.1% | 88.8% | (many diffs) |
| 6 | human | 6692 | SPINT1 | serine peptidase inhibitor,... | XM_011521958.2 | 91.6% | 91.6% | 940_941ins126;1101C>T;1404G>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1605
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccctgcgagg acgatggccc gcgcccgcct cgccccggcc ggcatccctg 121 ccgtcgcctt gtggcttctg tgcacgctcg gcctccaggg cacccaggcc gggccaccgc 181 ccgcgccccc tgggctgccc gcgggagccg actgcctgaa cagctttacc gccggggtgc 241 ctggcttcgt gctggacacc aacgcctcgg tcagcaacgg agctaccttc ctggagtccc 301 ccaccgtgcg ccggggctgg gactgcgtgc gcgcctgctg caccacccag aactgcaact 361 tggcgctagt ggagctgcag cccgaccgcg gggaggacgc catcgccgcc tgcttcctca 421 tcaactgcct ctacgagcag aacttcgtgt gcaagttcgc gcccagggag ggcttcatca 481 actacctcac gagggaagtg taccgctcct accgccagct gcggacccag ggctttggag 541 ggtctgggat ccccaaggcc tgggcaggca tagacttgaa ggtacaaccc caggaacccc 601 tggtgctgaa ggatgtggaa aacacagatt ggcgcctact gcggggtgac acggatgtca 661 gggtagagag gaaagaccca aaccaggtgg aactgtgggg actcaaggaa ggcacctacc 721 tgttccagct gacagtgact agctcagacc acccagagga cacggccaac gtcacagtca 781 ctgtgctgtc caccaagcag acagaagact actgcctcgc atccaacaag gtgggtcgct 841 gccggggctc tttcccacgc tggtactatg accccacgga gcagatctgc aagagtttcg 901 tttatggagg ctgcttgggc aacaagaaca actaccttcg ggaagaagag tgcattctag 961 cctgtcgggg tgtgcaaggc ccctccatgg aaaggcgcca tccagtgtgc tctggcacct 1021 gtcagcccac ccagttccgc tgcagcaatg gctgctgcat cgacagtttc ctggagtgtg 1081 acgacacccc caactgcccc gacgcctccg acgaggctgc ctgtgaaaaa tacacgagtg 1141 gctttgacga gctccagcgc atccatttcc ccagtgacaa agggcactgc gtggacctgc 1201 cagacacagg actctgcaag gagagcatcc cgcgctggta ctacaacccc ttcagcgaac 1261 actgcgcccg ctttacctat ggtggttgtt atggcaacaa gaacaacttt gaggaagagc 1321 agcagtgcct cgagtcttgt cgcggcatct ccaagaagga tgtgtttggc ctgaggcggg 1381 aaatccccat tcccagcaca ggctctgtgg agatggctgt cgcagtgttc ctggtcatct 1441 gcattgtggt ggtggtagcc atcttgggtt actgcttctt caagaaccag agaaaggact 1501 tccacggaca CCACCACCAC CCACCACCCA CCCCTGCCAG CTCCACTGTC TCCACTACCG 1561 AGGACACGGA GCACCTGGTC TATAACCACA CCACCCGGCC CCTCTGCCCA ACTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA AAGCCATATT TCATAACCAG GTTGACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt