Construct: ORF TRCN0000465389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016362.1_s317c1
- Derived from:
- ccsbBroadEn_09911
- DNA Barcode:
- TCACCAAACTGTGCCACGGGTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STEAP1B (256227)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256227 | STEAP1B | STEAP family member 1B | NM_207342.2 | 99.7% | 100% | 90A>G;363A>G |
| 2 | human | 256227 | STEAP1B | STEAP family member 1B | NM_001164460.1 | 70.7% | 69% | (many diffs) |
| 3 | human | 26872 | STEAP1 | STEAP family member 1 | NM_012449.3 | 69.9% | 66.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 801
- ORF length:
- 735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aagcagaaaa gacatcacaa accaagaaga aatttggaaa atgaagccta 121 ggagaaattt agaagacaac gattatttgc aaacggccca tgctgatgaa tttgactgcc 181 cttcagaact tcagcacgca caggaactct ttccacagtg gcacttgcca attaaaatag 241 ctgctgttat ggcatctctg acttttcttt acactcttct gagggaagta attcaccctt 301 tagcaacttc ccatcaacaa tatttttata aaattccaat cctggtcatc aacaaagtct 361 tgccaatggt ttccatcact ctcttggcat tggtttacct accaggtgtg atagcagcaa 421 ttgtccaggt tcataatgga accaagtata agaagtttcc acattggttG GATAAGTGGA 481 TGTTAACAAG AAAGCAGTTT GGGCTTCTCA GTTTGTTTTT TGCTGTACTG CATGCAATTT 541 ATACTCTGTC TTACGCAATG AGGCGATCCT ACAGATACAA GTTGCTAAAC TGGGCATATC 601 AACAGGTCCA ACAAAATAAA GAAGATGCCT GGATTGAGCA TGATGTTTGG AGAATGGAGA 661 TTTATGTGTC TCTGGGAATT GTGGGACTGG CAATACTGGC TCTGTTGGCT GTGACATCTA 721 TTCCATCTGT GAGTGACTCT TTGACATGGA GAGAATTTCA CTATATTCAG GTACATGGTA 781 GGATCAACTT CCTGACCTTG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAC CAAACTGTGC 961 CACGGGTCGA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt