Construct: ORF TRCN0000465392
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003325.1_s317c1
- Derived from:
- ccsbBroadEn_13712
- DNA Barcode:
- AATTCTTTGTGAAACAGAGTACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRR5-ARHGAP8 (553158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465392
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23779 | ARHGAP8 | Rho GTPase activating prote... | NM_181335.2 | 92.2% | 92.3% | 0_1ins108;132A>G |
| 2 | human | 23779 | ARHGAP8 | Rho GTPase activating prote... | NM_001017526.1 | 86.5% | 86.6% | 0_1ins108;132A>G;300_392del |
| 3 | human | 553158 | PRR5-ARHGAP8 | PRR5-ARHGAP8 readthrough | NM_181334.5 | 83% | 83.1% | 1_285del;525A>G |
| 4 | human | 23779 | ARHGAP8 | Rho GTPase activating prote... | NM_001198726.1 | 64.9% | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1476
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgatcctt cgggacaaga ttcgcttcta tgaggagctg cagagagaca 121 aggcggcggc ggctgctgtg ctgggtgcag tgaggaagag gccctcggtg gtgcccatgg 181 ctggccagga tcctgcgctg agcacgagtc acccgttcta cgacgtggcc agacatggca 241 ttctgcaggt ggcaggggat gaccgctttg gaagacgtgt tgtcacgttc agctgctgcc 301 ggatgccgcc ctcccacgag ctggaccacc agcggctgct ggagtatttg aagtacacac 361 tggaccaata cgttgagaac gattatacca tcgtctattt ccactacggg ctgaacagcc 421 ggaacaagcc ttccctgggc tggctccaga gcgcatacaa ggagttcgat aggaagtaca 481 agaagaactt gaaggccctc tacgtggtgc accccaccag cttcatcaag gtcctgtgga 541 acatcttgaa gcccctcatc agtcacaagt ttgggaagaa agtcatctat ttcaactacc 601 tgagtgagct ccacgaacac cttaaatacg accagctggt catccctccc gaagttttgc 661 ggtacgatga gaagctccag agcctgcacg agggccggac gccgcctccc accaagacac 721 caccgccgcg gcccccgctg cccacacagc agtttggcgt cagtctgcaa tacctcaaag 781 acaaaaatca aggcgaactc atcccccctg tgctgaggtt cacagtgacg tacctgagag 841 agaaaggcct gcgcaccgag ggcctgttcc ggagatccgc cagcgtgcag accgtccgcg 901 agatccagag gctctacaac caagggaagc ccgtgaactt tgacgactac ggggacattc 961 acatccctgc cgtgatcctg aagaccttcc tgcgagagct gccccagccg cttctgacct 1021 tccaggccta cgagcagatt ctcgggatca cctgtgtgga gagcagcctg cgtgtcactg 1081 gctgccgcca gatcttacgg agcctcccag agcacaacta cgtcgtccTC CGCTACCTCA 1141 TGGGCTTCCT GCATGCGGTG TCCCGGGAGA GCATCTTCAA CAAAATGAAC AGCTCTAACC 1201 TGGCCTGTGT CTTCGGGCTG AATTTGATCT GGCCATCCCA GGGGGTCTCC TCCCTGAGTG 1261 CCCTTGTGCC CCTGAACATG TTCACTGAAC TGCTGATCGA GTACTATGAA AAGATCTTCA 1321 GCACCCCGGA GGCACCTGGG GAGCACGGCC TGGCACCATG GGAACAGGGG AGCAGGGCAG 1381 CCCCTTTGCA GGAGGCTGTG CCACGGACAC AAGCCACGGG CCTCACCAAG CCTACCCTAC 1441 CTCCGAGTCC CCTGATGGCA GCCAGAAGAC GTCTCTTGCC AACTTTCTTG TACAAAGTGG 1501 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1561 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1621 AAATTCTTTG TGAAACAGAG TACATACGCG TTAAGTCgac aatcaacctc tggattacaa 1681 aatttgtgaa agatt