Transcript: Human NM_181334.5

Homo sapiens PRR5-ARHGAP8 readthrough (PRR5-ARHGAP8), mRNA.

Source:
NCBI, updated 2019-02-09
Taxon:
Homo sapiens (human)
Gene:
PRR5-ARHGAP8 (553158)
Length:
2161
CDS:
278..1972

Additional Resources:

NCBI RefSeq record:
NM_181334.5
NBCI Gene record:
PRR5-ARHGAP8 (553158)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_181334.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243370 GTTGCGAACACTCTGTATATT pLKO_005 1974 3UTR 100% 15.000 7.500 Y ARHGAP8 n/a
2 TRCN0000243369 ATACGTTGAGAACGATTATAC pLKO_005 862 CDS 100% 13.200 6.600 Y ARHGAP8 n/a
3 TRCN0000123103 CGATTATACCATCGTCTATTT pLKO.1 874 CDS 100% 13.200 6.600 Y PRR5-ARHGAP8 n/a
4 TRCN0000257012 GAGCTCCACGAACACCTTAAA pLKO_005 1100 CDS 100% 13.200 6.600 Y ARHGAP8 n/a
5 TRCN0000123101 TGTTGCGAACACTCTGTATAT pLKO.1 1973 CDS 100% 13.200 6.600 Y PRR5-ARHGAP8 n/a
6 TRCN0000243372 CCCTCATCAGTCACAAGTTTG pLKO_005 1047 CDS 100% 10.800 5.400 Y ARHGAP8 n/a
7 TRCN0000243371 TTTGGCGTCAGTCTGCAATAC pLKO_005 1247 CDS 100% 10.800 5.400 Y ARHGAP8 n/a
8 TRCN0000047358 CGGGACAAGATTCGCTTCTAT pLKO.1 575 CDS 100% 5.625 2.813 Y PRR5 n/a
9 TRCN0000195939 CGGGACAAGATTCGCTTCTAT pLKO.1 575 CDS 100% 5.625 2.813 Y PRR5 n/a
10 TRCN0000123100 CGGGAGAGCATCTTCAACAAA pLKO.1 1658 CDS 100% 5.625 2.813 Y PRR5-ARHGAP8 n/a
11 TRCN0000047362 GTGATCCTTCGGGACAAGATT pLKO.1 566 CDS 100% 5.625 2.813 Y PRR5 n/a
12 TRCN0000123102 GCGCATACAAGGAGTTCGATA pLKO.1 945 CDS 100% 4.950 2.475 Y PRR5-ARHGAP8 n/a
13 TRCN0000047361 CACCTGGAACAGCATCCACAA pLKO.1 400 CDS 100% 4.050 2.025 Y PRR5 n/a
14 TRCN0000123099 CACTCTGTATATTTCGAGCTA pLKO.1 1982 3UTR 100% 2.640 1.320 Y PRR5-ARHGAP8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_181334.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13712 pDONR223 100% 83% 83.1% None 1_285del;525A>G n/a
2 ccsbBroad304_13712 pLX_304 0% 83% 83.1% V5 1_285del;525A>G n/a
3 TRCN0000465392 AATTCTTTGTGAAACAGAGTACAT pLX_317 25% 83% 83.1% V5 1_285del;525A>G n/a
4 ccsbBroadEn_07932 pDONR223 100% 76.5% 76.5% None (many diffs) n/a
5 ccsbBroad304_07932 pLX_304 0% 76.5% 76.5% V5 (many diffs) n/a
6 TRCN0000480002 TGCTCAGTAATGGCGAGCAGCACT pLX_317 26.3% 76.5% 76.5% V5 (many diffs) n/a
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