Construct: ORF TRCN0000465394
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000979.1_s317c1
- Derived from:
- ccsbBroadEn_07704
- DNA Barcode:
- GGTAACAGCCCATGAAGGTGTATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUB1 (10923)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465394
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | NM_006713.4 | 99.7% | 99.2% | 377A>C |
| 2 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | XM_011513944.3 | 99.7% | 99.2% | 377A>C |
| 3 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | XM_017008986.1 | 99.7% | 99.2% | 377A>C |
| 4 | human | 10923 | SUB1 | SUB1 regulator of transcrip... | XM_017008987.1 | 99.7% | 99.2% | 377A>C |
| 5 | mouse | 20024 | Sub1 | SUB1 homolog (S. cerevisiae) | NM_011294.3 | 87.7% | 92.9% | (many diffs) |
| 6 | mouse | 20024 | Sub1 | SUB1 homolog (S. cerevisiae) | XM_006520042.3 | 87.7% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc taaatcaaag gaacttgttt cttcaagctc ttctggcagt gattctgaca 121 gtgaggttga caaaaagtta aagaggaaaa agcaagttgc tccagaaaaa cctgtaaaga 181 aacaaaagac aggtgagact tcgagagccc tgtcaTCTTC TAAACAGAGC AGCAGCAGCA 241 GAGATGATAA CATGTTTCAG ATTGGGAAAA TGAGGTACGT TAGTGTTCGC GATTTTAAAG 301 GCAAAGTGCT AATTGATATT AGAGAATATT GGATGGATCC TGAAGGTGAA ATGAAACCAG 361 GAAGAAAAGG TATTTCTTTA AATCCAGAAC AATGGAGCCA GCTGAAGGAA CAGATTTCTG 421 ACATTGATGA TGCAGTAAGA ACACTGTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 481 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 541 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGTAACAG 601 CCCATGAAGG TGTATGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 661 aagatt