Construct: ORF TRCN0000465395
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002359.1_s317c1
- Derived from:
- ccsbBroadEn_12382
- DNA Barcode:
- CCCGTTATTTACTGAGACAACGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL20A1 (57642)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465395
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | NM_020882.3 | 49.4% | 47.2% | (many diffs) |
| 2 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528940.1 | 48.9% | 46.7% | (many diffs) |
| 3 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528939.1 | 47.8% | 45.6% | (many diffs) |
| 4 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528938.1 | 46.8% | 44.7% | 1281A>G;1804_1807delCAGA;1916_4077del |
| 5 | human | 57642 | COL20A1 | collagen type XX alpha 1 chain | XM_011528937.1 | 44.5% | 42.5% | 1281A>G;1804_1807delCAGA;1916_4287del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1977
- ORF length:
- 1911
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctccggagac cctgcacacc tcggcctctg cctctggctg tggctgggcg 121 ccaccctggg aagagagcaa gttcaagcaa gcggtctcct gaggctggct gtgctgcctg 181 aggaccggct gcagatgaag tggagagagt cggaggggag cggcctcggc tacctggtgc 241 aggtgaagcc catggcaggg gactcggaac aggaggtgat actgaccacc aagaccccta 301 aggccacagt ggggggcctg agcccctcca agggctacac cttgcagatc ttcgagctca 361 ctggctctgg gcgcttcctg ctagctcgga gggagtttgt gattgaggat ctgaagagta 421 gctccctgga caggagcagc cagaggcccc tcggctctgg agccccggag cccaccccct 481 cccacacggg gagcccagac cctgagcagg cttctgagcc ccaagttgcc ttcacaccaa 541 gccaggatcc gcgcactcct gccggccccc agttccgctg cctgcccccc gtgcctgctg 601 acatggtctt cctggtggac gggtcctgga gcattggcca cagtcacttc cagcaggtca 661 aggacttcct ggccagtgtc atcgcaccct ttgaaatcgg gccggataag gtccaagtag 721 gcctgactca gtacagcggg gatgctcaga ctgagtggga cctgaactcc ctcagcacca 781 aggaacaggt gctggcagct gtgcgccgcc tccgctacaa gggggggaac acgttcacag 841 gccttgccct gacccacgtg ctggggcaga acctgcagcc ggcggctggc ctccgtccag 901 aggcagccaa ggtggtgatt ctggtgacgg acggcaagtc ccaggacgat gtgcacactg 961 ctgcccgtgt cctcaaggac ctgggcgtga acgtcttcgc tgtgggtgtg aagaacgccg 1021 atgaggctga gctgaggctc ctggcgtccc cgccgaggga catcaccgtc cacagcgtgc 1081 tggacttcct gcagctcggc gcgctggctg gcctgctcag ccgtctcatc tgccagaggc 1141 tccagggtgg gagcccgcgg cagggcccag cagcagcggc tccagccctg gacaccctcc 1201 ctgcccccac cagcctggtc ctgagccagg tgacctcctc cagcatccgc ctgtcctgga 1261 ctccagcccc ccggcacccc ctcaagtatc tgatcgtttg gcgagcctct agaggtggca 1321 cccccaggga ggtggtggtg gaggggcccg ccgcctccac ggagctgcac aacctggcct 1381 cccgcacaga gtacctggtc tccgtgttcc ccatctatga gggcggggtt ggcgaaggcc 1441 tgcggggcct ggtgaccaca gcacctctgc ctccgccccg ggcgctgacc ctggccgcag 1501 tgacgcccag aaccgtccac ctcacctggc agccctcggc cggggccacc cactacctgg 1561 tgcgatgttc tcctgcttcc cccaagggtg aagaggagga gcgagaggtg caggtcgggc 1621 ggcccgaggt gctgctggat ggcctggaac ctggcaggga ctatgaggtc tcggtgcaga 1681 gcctgcgagg ccctgagggc agcgaggccc ggggcatccg tgccaggacc cccaccctgg 1741 cccccccgag acacctgggc ttcTCAGACG TGAGCCACGA CGCGGCACGA GTGTTCTGGG 1801 AGGGTGCCCC GAGGCCTGTG CGCCTGGTCA GGGTCACCTA TGTGTCCAGC GAGGGTGGAC 1861 ACTCGGGGCA GAGGCTCCTG GGAACGCCAC CTCGGCCACG CTGGGGCCTC TCTCTTCCTC 1921 CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG GGTGGCTCCT CTACGCTGCC 1981 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2041 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2101 ATATATCTTG TGGAAAGGAC GACCCGTTAT TTACTGAGAC AACGACACGC GTTAAGTCga 2161 caatcaacct ctggattaca aaatttgtga aagatt