Construct: ORF TRCN0000465397
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006075.1_s317c1
- Derived from:
- ccsbBroadEn_00754
- DNA Barcode:
- CGTGTAGCACCTTCTGGCTCTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HMGN2 (3151)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465397
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3151 | HMGN2 | high mobility group nucleos... | NM_005517.4 | 100% | 100% | |
| 2 | human | 10473 | HMGN4 | high mobility group nucleos... | NM_006353.3 | 86.2% | 86.6% | (many diffs) |
| 3 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010172.2 | 86.2% | 86.6% | (many diffs) |
| 4 | human | 10473 | HMGN4 | high mobility group nucleos... | XM_017010173.2 | 86.2% | 86.6% | (many diffs) |
| 5 | human | 107986383 | LOC107986383 | uncharacterized LOC107986383 | XR_001742516.1 | 27.4% | (many diffs) | |
| 6 | human | 283651 | HMGN2P46 | high mobility group nucleos... | NR_022014.1 | 12.7% | (many diffs) | |
| 7 | mouse | 15331 | Hmgn2 | high mobility group nucleos... | NM_016957.3 | 93.3% | 96.6% | (many diffs) |
| 8 | mouse | 627375 | Gm6750 | predicted gene 6750 | XR_873085.1 | 58.7% | (many diffs) | |
| 9 | mouse | 100503799 | Gm7931 | predicted pseudogene 7931; ... | XR_868409.2 | 21.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 360
- ORF length:
- 270
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCCAAGAG AAAGGCTGAA GGGGATGCTA 121 AGGGAGATAA AGCAAAGGTG AAGGACGAAC CACAGAGAAG ATCCGCGAGG TTGTCTGCTA 181 AACCTGCTCC TCCAAAGCCA GAGCCCAAGC CTAAAAAGGC CCCTGCAAAG AAGGGAGAGA 241 AGGTACCCAA AGGGAAAAAG GGAAAAGCTG ATGCTGGCAA GGAGGGGAAT AACCCTGCAG 301 AAAATGGAGA TGCCAAAACA GACCAGGCAC AGAAAGCTGA AGGTGCTGGA GATGCCAAGT 361 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 421 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 481 TTTATATATC TTGTGGAAAG GACGACGTGT AGCACCTTCT GGCTCTCTAA CGCGTTAAGT 541 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt