Construct: ORF TRCN0000465403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010665.1_s317c1
- Derived from:
- ccsbBroadEn_03885
- DNA Barcode:
- TCTTATTACGTTTGTAATGTCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELOVL5 (60481)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60481 | ELOVL5 | ELOVL fatty acid elongase 5 | NM_001301856.2 | 100% | 100% | |
| 2 | human | 60481 | ELOVL5 | ELOVL fatty acid elongase 5 | NM_021814.5 | 100% | 100% | |
| 3 | human | 60481 | ELOVL5 | ELOVL fatty acid elongase 5 | NM_001242828.1 | 91.7% | 91.7% | 246_326del |
| 4 | human | 60481 | ELOVL5 | ELOVL fatty acid elongase 5 | NM_001242830.1 | 84.7% | 50% | 496_497ins125;773_786del |
| 5 | mouse | 68801 | Elovl5 | ELOVL family member 5, elon... | NM_134255.3 | 87.3% | 92.9% | (many diffs) |
| 6 | mouse | 68801 | Elovl5 | ELOVL family member 5, elon... | XM_006511400.1 | 87.3% | 92.9% | (many diffs) |
| 7 | mouse | 68801 | Elovl5 | ELOVL family member 5, elon... | XM_011242807.1 | 65.7% | 66.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 963
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga acattttgat gcatcactta gtacctattt caaggcattg ctaggccctc 121 gagatactag agtaaaagga tggtttcttc tggacaatta tatacccaca tttatctgct 181 ctgtcatata tttactaatt gtatggctgg gaccaaaata catgaggaat aaacagccat 241 tctcttgccg ggggatttta gtggtgtata accttggact cacactgctg tctctgtata 301 tgttctgtga gttagtaaca ggagtatggg aaggcaaata caacttcttc tgtcagggca 361 cacgcaccgc aggagaatca gatatgaaga ttatccgtgt cctctggtgg tactacttct 421 ccaaactcat agaatttatg gacactttct tcttcatcct gcgcaagaac aaccaccaga 481 tcacggtcct gcacgtctac caccatgcct cgatgctgaa catctggtgg tttgtgatga 541 actgggtccc ctgcggccac tcttattttg gtgccacact taatagcttc atccacgtcc 601 tcatgtactc ttactatggt ttgtcgtcag tcccttccat gcgtccatac ctctgGTGGA 661 AGAAGTACAT CACTCAGGGG CAGCTGCTTC AGTTTGTGCT GACAATCATC CAGACCAGCT 721 GCGGGGTCAT CTGGCCGTGC ACATTCCCTC TTGGTTGGTT GTATTTCCAG ATTGGATACA 781 TGATTTCCCT GATTGCTCTC TTCACAAACT TCTACATTCA GACCTACAAC AAGAAAGGGG 841 CCTCCCGAAG GAAAGACCAC CTGAAGGACC ACCAGAATGG GTCCATGGCT GCTGTGAATG 901 GACACACCAA CAGCTTTTCA CCCCTGGAAA ACAATGTGAA GCCAAGGAAG CTGCGGAAGG 961 ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGATC TTATTACGTT TGTAATGTCA AAACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt