Construct: ORF TRCN0000465411
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013650.1_s317c1
- Derived from:
- ccsbBroadEn_01989
- DNA Barcode:
- GTCTAATCTTTCATAAAAATGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEA15 (8682)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465411
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8682 | PEA15 | proliferation and apoptosis... | NM_001297577.2 | 100% | 100% | |
| 2 | human | 8682 | PEA15 | proliferation and apoptosis... | NM_003768.5 | 100% | 100% | |
| 3 | human | 8682 | PEA15 | proliferation and apoptosis... | XM_006711599.3 | 100% | 100% | |
| 4 | human | 8682 | PEA15 | proliferation and apoptosis... | NM_001297576.2 | 86% | 86% | 1_63del |
| 5 | human | 8682 | PEA15 | proliferation and apoptosis... | NM_001297578.2 | 83% | 82.3% | 106_107ins66 |
| 6 | human | 8682 | PEA15 | proliferation and apoptosis... | NR_123724.2 | 12.6% | (many diffs) | |
| 7 | mouse | 18611 | Pea15a | phosphoprotein enriched in ... | NM_001329869.1 | 94.8% | 99.2% | (many diffs) |
| 8 | mouse | 18611 | Pea15a | phosphoprotein enriched in ... | NM_011063.3 | 94.8% | 99.2% | (many diffs) |
| 9 | mouse | 18611 | Pea15a | phosphoprotein enriched in ... | XM_006496702.1 | 94.8% | 99.2% | (many diffs) |
| 10 | mouse | 18611 | Pea15a | phosphoprotein enriched in ... | NM_001329871.1 | 78.4% | 81.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgagtacggg acccTCCTGC AAGACCTGAC CAACAACATC ACCCTTGAAG 121 ATCTAGAACA GCTCAAGTCG GCCTGCAAGG AAGACATCCC CAGCGAAAAG AGTGAGGAGA 181 TCACTACTGG CAGTGCCTGG TTTAGCTTCC TGGAGAGCCA CAACAAGCTG GACAAAGACA 241 ACCTCTCCTA CATTGAGCAC ATCTTTGAGA TCTCCCGCCG TCCTGACCTA CTCACTATGG 301 TGGTTGACTA CAGAACCCGT GTGCTGAAGA TCTCTGAGGA GGATGAGCTG GACACCAAGC 361 TAACCCGTAT CCCCAGTGCC AAGAAGTACA AAGACATTAT CCGGCAGCCC TCTGAGGAAG 421 AGATCATCAA ATTGGCTCCC CCACCGAAGA AGGCCTGCCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AGTCTAATCT TTCATAAAAA TGCCGACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt