Construct: ORF TRCN0000465449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002889.1_s317c1
- Derived from:
- ccsbBroadEn_03853
- DNA Barcode:
- CCCTTGGCCACCCGTCCCCTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSM2 (57819)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465449
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57819 | LSM2 | LSM2 homolog, U6 small nucl... | NM_021177.5 | 100% | 100% | |
| 2 | mouse | 27756 | Lsm2 | LSM2 homolog, U6 small nucl... | NM_001110101.2 | 92.2% | 100% | (many diffs) |
| 3 | mouse | 27756 | Lsm2 | LSM2 homolog, U6 small nucl... | NM_001204274.1 | 68.2% | 57.5% | (many diffs) |
| 4 | mouse | 27756 | Lsm2 | LSM2 homolog, U6 small nucl... | NM_030597.3 | 66.6% | 71.7% | (many diffs) |
| 5 | mouse | 27756 | Lsm2 | LSM2 homolog, U6 small nucl... | NM_001204273.1 | 25.5% | 19.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 351
- ORF length:
- 285
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cttctattct tttttcaagt cccttgtggg caaggatgtg gtcgtGGAAC 121 TAAAGAATGA CCTGAGCATC TGTGGAACCC TCCATTCTGT GGATCAGTAT CTCAACATCA 181 AACTAACTGA CATCAGTGTC ACAGACCCTG AGAAATACCC TCACATGTTA TCAGTGAAGA 241 ACTGCTTCAT TCGGGGCTCA GTGGTCCGAT ACGTGCAGCT GCCAGCAGAT GAGGTCGACA 301 CACAGTTGCT ACAGGATGCG GCAAGGAAGG AAGCCCTGCA GCAGAAACAG TGCCCAACTT 361 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 421 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 481 CTTGTGGAAA GGACGACCCT TGGCCACCCG TCCCCTTTTA ACGCGTTAAG TCgacaatca 541 acctctggat tacaaaattt gtgaaagatt