Transcript: Mouse NM_030597.3

Mus musculus LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated (Lsm2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-20
Taxon:
Mus musculus (mouse)
Gene:
Lsm2 (27756)
Length:
896
CDS:
128..523

Additional Resources:

NCBI RefSeq record:
NM_030597.3
NBCI Gene record:
Lsm2 (27756)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_030597.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000294935 TAGTCGTGGAACTCAAGAATG pLKO_005 279 CDS 100% 10.800 15.120 N Lsm2 n/a
2 TRCN0000123801 GACCAGTACCTCAATATCAAA pLKO.1 332 CDS 100% 5.625 7.875 N Lsm2 n/a
3 TRCN0000123799 CCCAATACTTAAGGTGTGTTT pLKO.1 602 3UTR 100% 4.950 6.930 N Lsm2 n/a
4 TRCN0000287415 CCCAATACTTAAGGTGTGTTT pLKO_005 602 3UTR 100% 4.950 6.930 N Lsm2 n/a
5 TRCN0000294894 GTGCTGTTGACGAGGACTTTG pLKO_005 213 CDS 100% 10.800 7.560 N Lsm2 n/a
6 TRCN0000123800 CCCTCACATGTTATCAGTCAA pLKO.1 388 CDS 100% 4.950 3.465 N Lsm2 n/a
7 TRCN0000287408 CCCTCACATGTTATCAGTCAA pLKO_005 388 CDS 100% 4.950 3.465 N Lsm2 n/a
8 TRCN0000074679 CCCTGAGAAATACCCTCACAT pLKO.1 376 CDS 100% 4.950 3.465 N LSM2 n/a
9 TRCN0000123802 GACACACAACTGCTACAGGAT pLKO.1 467 CDS 100% 2.640 1.848 N Lsm2 n/a
10 TRCN0000123803 CCTCAATATCAAACTAACCGA pLKO.1 340 CDS 100% 0.750 0.525 N Lsm2 n/a
11 TRCN0000287409 CCTCAATATCAAACTAACCGA pLKO_005 340 CDS 100% 0.750 0.525 N Lsm2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030597.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03853 pDONR223 100% 66.6% 71.7% None (many diffs) n/a
2 ccsbBroad304_03853 pLX_304 0% 66.6% 71.7% V5 (many diffs) n/a
3 TRCN0000465449 CCCTTGGCCACCCGTCCCCTTTTA pLX_317 80.6% 66.6% 71.7% V5 (many diffs) n/a
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