Construct: ORF TRCN0000465451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004169.1_s317c1
- Derived from:
- ccsbBroadEn_15777
- DNA Barcode:
- ACCGAGGTCTATATCCGGGTCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RWDD3 (25950)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465451
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001278247.1 | 99.8% | 99.4% | 213T>G |
| 2 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001199682.1 | 90.5% | 83.5% | (many diffs) |
| 3 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001128142.1 | 81.4% | 72.1% | (many diffs) |
| 4 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_001278248.1 | 73.2% | 67.4% | 213T>G;481_527del;603_756del |
| 5 | human | 25950 | RWDD3 | RWD domain containing 3 | NM_015485.5 | 67.6% | 57.6% | (many diffs) |
| 6 | human | 25950 | RWDD3 | RWD domain containing 3 | NR_103483.1 | 42.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 624
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttctttcc tgtggttccc tgcaccagag aggacccgag acagatggga 121 ccgtgttcag aattcacaca aaagctgaag gatttatgga tgcggatata cctctggaat 181 tggtgttcca tttgccagtc aattatcctt catgtctacc tggtatctcg attaactctg 241 aacagttgac cagggcccag tgtgtgactg tgaaagagaa gttacttgag caagcagaga 301 gccttttgtc ggagcctatg gttcatgagc tggttctctg gattcagcag aatctcaggc 361 atatccTCAG CCAACCAGAA ACTGGCAGTG GCAGTGAAAA GTGTACTTTT TCAACAAGCA 421 CGACCATGGA TGATGGATTG TGGATAACTC TTTTGCATTT AGATCACATG AGAGCAAAGA 481 CTAAATATGT CAAAATTGTG GAGAAGTGGG CTTCAGATTT AAGGCTGACA GGAAGACTGA 541 TGTTCATGGG AGTACTTGAT TCTTCAGAAA ACCTCCAAAG TAGATGTGGA CTCAAGTGGA 601 AAGAAATGCA AAGAGAAAAT GATTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 661 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 721 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CCGAGGTCTA 781 TATCCGGGTC AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 841 att