Construct: ORF TRCN0000465485
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002730.1_s317c1
- Derived from:
- ccsbBroadEn_15815
- DNA Barcode:
- AAACATGAGATCAGGTGTTCACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPOCK3 (50859)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465485
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001040159.2 | 99.9% | 100% | 147T>C |
| 2 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | XM_017008258.1 | 99.9% | 100% | 147T>C |
| 3 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_016950.2 | 99.2% | 99.3% | 147T>C;190_198delGAAGTAGAG |
| 4 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | XM_011532018.1 | 99.2% | 99.3% | 147T>C;190_198delGAAGTAGAG |
| 5 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | XM_017008257.1 | 97.2% | 97.3% | 1_36del;183T>C |
| 6 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001204356.1 | 90.6% | 90.5% | 147T>C;589_590ins120 |
| 7 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001204352.1 | 87% | 85.9% | (many diffs) |
| 8 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001204354.1 | 79.4% | 79.4% | 0_1ins267 |
| 9 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001204355.1 | 78.4% | 72.7% | 0_1ins118;29T>C;71_72ins161 |
| 10 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001251967.1 | 77.9% | 78% | 147T>C;189_190ins285 |
| 11 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001204353.1 | 72.9% | 72.9% | 0_1ins351 |
| 12 | human | 50859 | SPOCK3 | SPARC (osteonectin), cwcv a... | NM_001204358.2 | 71.6% | 71.5% | (many diffs) |
| 13 | mouse | 72902 | Spock3 | sparc/osteonectin, cwcv and... | NM_001252621.1 | 89.3% | 90% | (many diffs) |
| 14 | mouse | 72902 | Spock3 | sparc/osteonectin, cwcv and... | XM_006509518.1 | 89.3% | 90% | (many diffs) |
| 15 | mouse | 72902 | Spock3 | sparc/osteonectin, cwcv and... | NM_001252620.1 | 88.7% | 89.4% | (many diffs) |
| 16 | mouse | 72902 | Spock3 | sparc/osteonectin, cwcv and... | NM_023689.3 | 88.7% | 89.4% | (many diffs) |
| 17 | mouse | 72902 | Spock3 | sparc/osteonectin, cwcv and... | XM_006509517.2 | 86.1% | 86.8% | (many diffs) |
| 18 | mouse | 72902 | Spock3 | sparc/osteonectin, cwcv and... | XM_006509516.2 | 85.6% | 86.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1365
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct caaggtgtca gccgtactgt gtgtgtgtgc agccgcttgg tgcagtcagt 121 ctctcgcagc tgccgcggcg gtggctgcag ccggggggcg gtcggacggc ggtaattttc 181 tggatgataa acaatggctc accacaatct cccagtatga caaggaagtc ggacagtgga 241 acaaattccg agacgatgat tatttccgca cttggagtcc aggaaaaccc ttcgatcagg 301 ctttagatcc agctaaggat ccatgcttaa agatgaaatg tagtcgccat aaagtatgca 361 ttgctcaaga ttctcagact gcagtctgca ttagtcaccg gaggcttaca cacaggatga 421 aagaagcagg agtagaccat aggcagtgga ggggtcccat attatccacc tgcaagcagt 481 gcccagtggt ctatcccagc cctgtttgtg gttcagatgg tcatacctac tcttttcagt 541 gcaaactaga atatcaggca tgtgtcttag gaaaacagat ctcagtcaaa tgtgaaggac 601 attgcccatg tccttcagat aagcccacca gtacaagcag aaatgttaag agagcatgca 661 gtgacctgga gttcagggaa gtggcaaaca gattgcggga ctggttcaag gcccttcatg 721 aaagtggaag tcaaaacaag aagacaaaaa cattgctgag gcctgagaga agcagattcg 781 ataccagcat cttgccaatt tgcaaggact cacttggctg gatgtttaac agacttgata 841 caaactatga cctgctattg gaccagtcag agctcagaag catttacctt gataagaatg 901 aacagtgtac caaggcattc ttcaattctt gtgacacata caaggacagt ttaatatcta 961 ataatgagtg gTGCTACTGC TTCCAGAGAC AGCAAGACCC ACCTTGCCAG ACTGAGCTCA 1021 GCAATATTCA GAAGCGGCAA GGGGTAAAGA AGCTCCTAGG ACAGTATATC CCCCTGTGTG 1081 ATGAAGATGG TTACTACAAG CCAACACAAT GTCATGGCAG TGTTGGACAG TGCTGGTGTG 1141 TTGACAGATA TGGAAATGAA GTCATGGGAT CCAGAATAAA TGGTGTTGCA GATTGTGCTA 1201 TAGATTTTGA GATCTCCGGA GATTTTGCTA GTGGCGATTT TCATGAATGG ACTGATGATG 1261 AGGATGATGA AGACGATATT ATGAATGATG AAGATGAAAT TGAAGATGAT GATGAAGATG 1321 AAGGGGATGA TGATGATGGT GGTGATGACC ATGATGTATA CATTTGCCCA ACTTTCTTGT 1381 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1441 AGTAATGAAC TAGTCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 1501 AAAGGACGAA AACATGAGAT CAGGTGTTCA CATACGCGTT AAGTCgacaa tcaacctctg 1561 gattacaaaa tttgtgaaag att