Construct: ORF TRCN0000465495
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008372.1_s317c1
- Derived from:
- ccsbBroadEn_11072
- DNA Barcode:
- CAATGGGAACAACATCACCCATAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTMS (5763)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465495
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5763 | PTMS | parathymosin | NM_001330333.2 | 92.4% | 88.4% | (many diffs) |
| 2 | human | 5763 | PTMS | parathymosin | NM_002824.6 | 55.6% | 24.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 396
- ORF length:
- 306
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCCCAGGAG AGAGAGTCCT CAGGAAAAGG 121 CCCCAGAGGA CCTGAAGGAG AAGAAGGAGA AGGTGGAGGA GAAGGCAAGC CGGAAAGAGC 181 GAAAGAAAGA AGTGGTGGAG GCTCCAGTCC CATTGGTGGT GATGGTGGGC AAGGCATGCA 241 GCCAGCCTGA GGCTACTCCC TCTCCTGGTC CCCACAGGAG GAGGAGAACG GGGCTGAGGA 301 GGAAGAAGAA GAAACTGCCG AGGATGGAGA GGAGGAAGAT GAAGGGGAAG AAGAAGATGA 361 GGAAGAAGAA GAAGAGGATG ATGAAGGGCC CGCGCTGCCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ACAATGGGAA CAACATCACC CATAGACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt