Construct: ORF TRCN0000465506
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005081.3_s317c1
- Derived from:
- ccsbBroadEn_15130
- DNA Barcode:
- AACGAACAACTGCCAGGCCTATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBKS (64080)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465506
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64080 | RBKS | ribokinase | NM_022128.3 | 100% | 100% | |
| 2 | human | 64080 | RBKS | ribokinase | NM_001287580.1 | 79.1% | 79.1% | 0_1ins201 |
| 3 | mouse | 71336 | Rbks | ribokinase | NM_153196.1 | 85.3% | 86% | (many diffs) |
| 4 | mouse | 71336 | Rbks | ribokinase | XM_006504114.1 | 71.1% | 71.5% | (many diffs) |
| 5 | mouse | 71336 | Rbks | ribokinase | XM_006504113.3 | 70% | 69.6% | (many diffs) |
| 6 | mouse | 71336 | Rbks | ribokinase | XR_376828.2 | 61.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgtctggg gaaccccaga ggcagtggca agaggaggtg gcggcggtgg 121 tagtggtggg ctcctgcatg accgacctgg tcagtcttac ttctcgtttg ccaaaaactg 181 gagaaaccat ccatggacat aagtttttta ttggctttgg agggaaaggt gccaaccagt 241 gtgtccaagc tgctcggctt ggagcaatga cgtccatggt gtgtaaggtt ggcaaagatt 301 cttttggcaa tgattatata gaaaacttaa aacagaatga tatttctaca gaatttacat 361 atcagactaa agatgctgct acaggaactg cttctataat tgtcaataat gaaggccaga 421 atatcattgt catagtggct ggagcaaatt tacttttgaa tacggaggat ctgagggcag 481 cagccaatgt cattagcaga gccaaagtca tggtctgcca gctcgaaata actccagcaa 541 cttctttgga agccctaaca atggcccgca ggagtggagt gaaaaccttg ttcaatccag 601 cccctgccat tgctgacctg gatccccagt tctacaccct ctcagatgtg ttctgctgca 661 atgaaagtga ggctgagatt ttaactggcc tcacggtggg cagcgctgca gatgctgggg 721 aggctgcatt agtgctcttg aaaaggggct gccaggtggt aatcattacc ttaggggctg 781 aaggatgtgt ggtgctgtca cagacagaac ctgagcCAAA GCACATTCCC ACAGAGAAAG 841 TCAAGGCTGT GGATACCACG GGTGCTGGTG ACAGCTTTGT GGGAGCTCTG GCCTTCTACC 901 TGGCTTACTA TCCAAATCTG TCCTTGGAAG ACATGCTCAA CAGATCCAAT TTCATTGCAG 961 CAGTCAGTGT CCAGGCTGCA GGAACACAGT CATCTTACCC TTACAAAAAA GACCTTCCGC 1021 TTACTCTGTT TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAC GAACAACTGC CAGGCCTATT 1201 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t